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Protein

Acetate kinase

Gene

ackA

Organism
Mycobacterium marinum (strain ATCC BAA-535 / M)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. no protein annotated in this organism
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi14MagnesiumUniRule annotation1
Binding sitei21ATPUniRule annotation1
Binding sitei80SubstrateUniRule annotation1
Active sitei137Proton donor/acceptorUniRule annotation1
Sitei169Transition state stabilizerUniRule annotation1
Sitei230Transition state stabilizerUniRule annotation1
Metal bindingi373MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 201ATPUniRule annotation5
Nucleotide bindingi271 – 273ATPUniRule annotation3
Nucleotide bindingi319 – 323ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:MMAR_0711
OrganismiMycobacterium marinum (strain ATCC BAA-535 / M)
Taxonomic identifieri216594 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000001190 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004219491 – 388Acetate kinaseAdd BLAST388

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi216594.MMAR_0711.

Structurei

Secondary structure

1388
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 15Combined sources7
Beta strandi20 – 25Combined sources6
Turni27 – 29Combined sources3
Beta strandi32 – 38Combined sources7
Helixi51 – 64Combined sources14
Helixi69 – 71Combined sources3
Beta strandi74 – 82Combined sources9
Turni85 – 87Combined sources3
Helixi96 – 105Combined sources10
Helixi106 – 108Combined sources3
Turni110 – 112Combined sources3
Helixi113 – 126Combined sources14
Beta strandi132 – 136Combined sources5
Helixi139 – 143Combined sources5
Helixi146 – 149Combined sources4
Helixi155 – 160Combined sources6
Helixi170 – 184Combined sources15
Helixi188 – 190Combined sources3
Beta strandi192 – 208Combined sources17
Beta strandi211 – 216Combined sources6
Beta strandi223 – 225Combined sources3
Helixi236 – 244Combined sources9
Helixi250 – 259Combined sources10
Helixi262 – 267Combined sources6
Helixi272 – 280Combined sources9
Helixi284 – 308Combined sources25
Beta strandi313 – 317Combined sources5
Helixi318 – 323Combined sources6
Helixi325 – 332Combined sources8
Helixi336 – 338Combined sources3
Helixi344 – 348Combined sources5
Beta strandi353 – 356Combined sources4
Beta strandi363 – 368Combined sources6
Helixi373 – 383Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DQ8X-ray2.25A/B1-387[»]
ProteinModelPortaliB2HPZ3.
SMRiB2HPZ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288399.
KOiK00925.
OMAiHRLKKYI.
OrthoDBiPOG091H02K4.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiIPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2HPZ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASRPNRVV LVLNSGSSSL KFQLVEPDSG MSRATGNIER IGEESSSVPD
60 70 80 90 100
HDAALRRVFE ILAEDDIDLQ SCGLVAVGHR VVHGGKDFYE PTLLNDAVIG
110 120 130 140 150
KLDELSPLAP LHNPPAVLCI RVARALLPDV PHIAVFDTAF FHQLPPAAAT
160 170 180 190 200
YAIDRELADV WKIRRYGFHG TSHEYVSQQA AEFLGKPIGD LNQIVLHLGN
210 220 230 240 250
GASASAVAGG RPVETSMGLT PLEGLVMGTR SGDLDPGVIG YLWRTAKLGV
260 270 280 290 300
DEIESMLNHR SGMLGLAGER DFRRLRAMID DGDPAAELAY DVFIHRLRKY
310 320 330 340 350
VGAYLAVLGH TDVVSFTAGI GEHDAAVRRD TLAGMAELGI SLDERRNACP
360 370 380
SGGARRISAD DSPVTVLVIP TNEELAIARH CCSVLVAV
Length:388
Mass (Da):41,617
Last modified:June 10, 2008 - v1
Checksum:iBE4CD8FAACB8734A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA. Translation: ACC39170.1.
RefSeqiWP_012392661.1. NC_010612.1.

Genome annotation databases

EnsemblBacteriaiACC39170; ACC39170; MMAR_0711.
KEGGimmi:MMAR_0711.
PATRICi18062139. VBIMycMar75906_0762.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA. Translation: ACC39170.1.
RefSeqiWP_012392661.1. NC_010612.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DQ8X-ray2.25A/B1-387[»]
ProteinModelPortaliB2HPZ3.
SMRiB2HPZ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216594.MMAR_0711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC39170; ACC39170; MMAR_0711.
KEGGimmi:MMAR_0711.
PATRICi18062139. VBIMycMar75906_0762.

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288399.
KOiK00925.
OMAiHRLKKYI.
OrthoDBiPOG091H02K4.

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiIPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACKA_MYCMM
AccessioniPrimary (citable) accession number: B2HPZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: June 10, 2008
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.