Reviewed, UniProtKB/Swiss-Prot B2HNJ0 (PLSB_MYCMM)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	MMAR_3833

Organism

Mycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]

Taxonomic identifier

216594 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	788 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00393.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity. HAMAP MF_00393
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 788	788	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000123085
Regions
	Motif	271 – 276	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	B2HNJ0-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 4367AF048DA614E3	FASTA	788	88,236
	10 20 30 40 50 60 MTKPVADTSA VLTSEDTLVL ASMGSPVEMQ LIMDWLGEQR ARTPGAKFDV LKLPPRNAPT 70 80 90 100 110 120 AALTALVEQL ESGSEAGADR SIVPVQVFWQ PPADRSRLAK VAGLLPGRDP YHPNPRQQRR 130 140 150 160 170 180 ILRSDPQRAR VMAGESATVS ELRKQWRDNT VGEDQHDFAH FVARRAILAL ARAEYRILGP 190 200 210 220 230 240 QYKSPRLVKP EMLASARFRA GLEKIPGATV EEAGKMLDEL ATGWSQASVD VFSVLGRLIS 250 260 270 280 290 300 RGFDPEFDYD EYQVAAMRTA LEAHPAVLLF SHRSYIDGAV VPVAMQDNRL PPVHMFGGVN 310 320 330 340 350 360 LSFGVMGPLM RRAGMIFIRR NIAGDPLYKY VLKEYVGYVV EKRFNLSWSI EGTRSRTGKM 370 380 390 400 410 420 LPPKLGLMSY VADAYLDGRS DDILLQGVSI CFDQLHEIAE YAAYARGAEK TPEGFSWLYN 430 440 450 460 470 480 FIKAQGERNY GKIYVRFPEA VSMRQYLGVP HGPITHDLAA KRLALQKMSF EVAWRILRAT 490 500 510 520 530 540 PMTATGLVCA LLLTARGTAL TLGQLHHTLQ DSLDYLERKQ TPMSTSALRL RSREGVRAAV 550 560 570 580 590 600 DALSNAHPVT RVDSGREPVW YIAPEDELAA AFYRNSVIHA FLETSIVELA LAHARHAEGD 610 620 630 640 650 660 RMAAFWDQVM RLRDLLKFDF YFADSAAFRA NIAEEMAWHR DWESQVAAGG DQIDAILYAK 670 680 690 700 710 720 RPLISDAMLR VFFEAYAIVA DVLRDAPANI KQKDLTDLAL GLGRQYVAQA RVRSSEPVST 730 740 750 760 770 780 LLFATARQVV DDQHLIEPAP DLTERRSAFL AELRNILRDF DYVGKIARNR FVARELKARQ ERLEQQAQ

References

[1]

"Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis." Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K., Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C., Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K., Lord A., Moule S. , Mungall K., Norbertczak H., Quail M.A., Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R., Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T. Genome Res. 18:729-741(2008) [PubMed: 18403782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000854 Genomic DNA. Translation: ACC42243.1.
RefSeq	YP_001852098.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6228107.
GenomeReviews	Gene locus MMAR_3833 in contig CP000854_GR.
KEGG	mmi:MMAR_3833.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG565163.
OMA	LEAAFYR.
Family and domain databases
HAMAP	MF_00393. Glyc3P_acyltrans. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_MYCMM

Accession

Primary (citable) accession number: B2HNJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 19, 2010
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)