B2HME9 (PROA_MYCMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	MMAR_3753

Organism

Mycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]

Taxonomic identifier

216594 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_1000193628

Sequences

	Sequence		Length	Mass (Da)
	B2HME9 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 2E710B12F6BCE85B	FASTA	423	44,566
	10 20 30 40 50 60 MTLQAAPRSA AAQQREPDLR QEVHDAARRA RVAARLLAVV PTGVKDRALH AAADAILAHV 70 80 90 100 110 120 DRILSANAED LDAARAADTP AAMLDRLALN PQRVDGIAAG LRQVAGLPDP VGEVLRGYTL 130 140 150 160 170 180 PNGLQLRQQR VPLGVVGMIY EGRPNVTVDA FGLTLKSGNA ALLRGSSSAA RSNEALVNVL 190 200 210 220 230 240 RSALDSEQLP ADAVQLLSSA DRSTVTHLIQ ARGLVDVAIP RGGAGLIDAV VRDAQVPTIE 250 260 270 280 290 300 TGVGNCHVYV HEAADLDVAE RILLNSKTRR PSVCNAAETL LVDAAIAEHA MPRLIGALQD 310 320 330 340 350 360 AGVTVHLDAD EQDLRREYLA MEIAVAVVDG VDGAIAHINE YGTGHTEAIV TTNLAAAQRF 370 380 390 400 410 420 TERIDAAAVM VNASTAFTDG EQFGFGAEIG ISTQKLHARG PMGLPELTST KWIVWGEGHT RPA

References

[1]

"Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis." Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K., Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C., Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K., Lord A., Moule S. , Mungall K., Norbertczak H., Quail M.A., Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R., Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T. Genome Res. 18:729-741(2008) [PubMed: 18403782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-535 / M.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000854 Genomic DNA. Translation: ACC42169.1.
RefSeq	YP_001852024.1. NC_010612.1.
3D structure databases
ProteinModelPortal	B2HME9.
ModBase	Search...
Protein-protein interaction databases
STRING	B2HME9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000034052; EBMYCP00000032492; EBMYCG00000034047.
GeneID	6228026.
GenomeReviews	Gene locus MMAR_3753 in contig CP000854_GR.
KEGG	mmi:MMAR_3753.
PATRIC	18068671. VBIMycMar75906_4012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000015663.
HOGENOM	HBG318080.
OMA	QYPAACN.
ProtClustDB	PRK00197.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 2 hits. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_MYCMM

Accession

Primary (citable) accession number: B2HME9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program