Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA dimethylallyltransferase 2

Gene

miaA2

Organism
Mycobacterium marinum (strain ATCC BAA-535 / M)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMMAR216594:GJOB-1997-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferase 2UniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase 2UniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferase 2UniRule annotation
Short name:
DMATase 2UniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferase 2UniRule annotation
Short name:
IPP transferase 2UniRule annotation
Short name:
IPPT 2UniRule annotation
Short name:
IPTase 2UniRule annotation
Gene namesi
Name:miaA2UniRule annotation
Ordered Locus Names:MMAR_1986
OrganismiMycobacterium marinum (strain ATCC BAA-535 / M)
Taxonomic identifieri216594 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000001190 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314tRNA dimethylallyltransferase 2PRO_0000377228Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei103 – 1031Interaction with substrate tRNAUniRule annotation
Sitei124 – 1241Interaction with substrate tRNAUniRule annotation

Protein-protein interaction databases

STRINGi216594.MMAR_1986.

Structurei

3D structure databases

ProteinModelPortaliB2HL15.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 156Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiAEMDGIP.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

B2HL15-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPLAIIGPT GSGKSQLALD VAERLAGEVP AEIVNADAMQ LYRGMDIGTA
60 70 80 90 100
KLSVAARRGI PHHQLDVLNV AETATVARYQ QAAAADIEAI IARGHVPIVV
110 120 130 140 150
GGSMLYVQSL LDNWSFPATD PAVRARWEEC LAELGVGELH AELARRDPAA
160 170 180 190 200
AATILPTDGR RIVRALEVIE LTGQPFAASA PRIGAAQWGT AIIGLDCDTP
210 220 230 240 250
ILDDRLAVRT DSMFERGLVE EVRVLLRAGL RDGVTAARAL GYAQVLAALD
260 270 280 290 300
AGGGAELLDD AREQTYFGTR RYVRRQRSWF RRDHRVHWCD AGATGPSDRA
310
AMVDEALRVW RHVT
Length:314
Mass (Da):33,965
Last modified:June 10, 2008 - v1
Checksum:i2C5B247598C3D02F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA. Translation: ACC40436.1.
RefSeqiWP_012393771.1. NC_010612.1.

Genome annotation databases

EnsemblBacteriaiACC40436; ACC40436; MMAR_1986.
KEGGimmi:MMAR_1986.
PATRICi18064871. VBIMycMar75906_2120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA. Translation: ACC40436.1.
RefSeqiWP_012393771.1. NC_010612.1.

3D structure databases

ProteinModelPortaliB2HL15.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216594.MMAR_1986.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC40436; ACC40436; MMAR_1986.
KEGGimmi:MMAR_1986.
PATRICi18064871. VBIMycMar75906_2120.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiAEMDGIP.
OrthoDBiPOG091H00NK.

Enzyme and pathway databases

BioCyciMMAR216594:GJOB-1997-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA2_MYCMM
AccessioniPrimary (citable) accession number: B2HL15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: June 10, 2008
Last modified: September 7, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.