B2HKY5 (B2HKY5_MYCMM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Thymidylate synthase HAMAP MF_00008
Short name=TS HAMAP MF_00008
Short name=TSase HAMAP MF_00008
EC=2.1.1.45 HAMAP MF_00008

Gene names

Name:	thyA HAMAP MF_00008 EMBL ACC40406.1
Ordered Locus Names:	MMAR_1956

Organism

Mycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]

Taxonomic identifier

216594 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451
Subunit structure	Homodimer By similarity. HAMAP MF_00008 SAAS SAAS023451
Subcellular location	Cytoplasm By similarity HAMAP MF_00008.
Sequence similarities	Belongs to the thymidylate synthase family. RuleBase RU004150 Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily. HAMAP MF_00008

Ontologies

Keywords
Biological process	Nucleotide biosynthesis HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451
Cellular component	Cytoplasm HAMAP MF_00008
Molecular function	Methyltransferase HAMAP MF_00008 RuleBase RU000500 SAAS SAAS023451 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	149	1	By similarity HAMAP MF_00008

Sequences

	Sequence		Length	Mass (Da)
	B2HKY5 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: A1EA5C0A55F0D0B1	FASTA	266	30,036
	10 20 30 40 50 60 MPIATPYEDL LRRVLETGTA KSDRTGTGTR SLFGQQIRYD LGCGFPLLTT KKVHFKSVVY 70 80 90 100 110 120 ELLWFLRGDS NVGWLQQHGV TIWDEWASET GDLGPIYGVQ WRSWPTPSGE HIDQISAALE 130 140 150 160 170 180 LLRTDPDSRR IIVSAWNVGQ IPQMALPPCH AFFQFYVADG RLSCQLYQRS ADLFLGVPFN 190 200 210 220 230 240 IASYALLTHM MAAQAGLSVG EFIWTGGDCH IYGNHVEQVT EQLRREPRPY PKLSLSQRDS 250 260 IFDYTYEDVV VQDYDPHPAI KAPVAV

References

[1]

"Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis." Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K., Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C., Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K., Lord A., Moule S. , Mungall K., Norbertczak H., Quail M.A., Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R., Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T. Genome Res. 18:729-741(2008) [PubMed: 18403782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000854 Genomic DNA. Translation: ACC40406.1.
RefSeq	YP_001850261.1. NC_010612.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2HKY5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000031981; EBMYCP00000030421; EBMYCG00000031976.
GeneID	6226216.
GenomeReviews	Gene locus MMAR_1956 in contig CP000854_GR.
KEGG	mmi:MMAR_1956.
PATRIC	18064811. VBIMycMar75906_2090.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017116.
HOGENOM	HBG588098.
OMA	RMALAPC.
ProtClustDB	PRK01827.
Family and domain databases
HAMAP	MF_00008. Thymidy_synth_bact. [Tree]
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KO	K00560.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. Thym_sym. 2 hits.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B2HKY5_MYCMM

Accession

Primary (citable) accession number: B2HKY5

Entry history

Integrated into UniProtKB/TrEMBL:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)