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B2HJ79 (B2HJ79_MYCMM) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518
Gene names
Name:topA EMBL ACC43542.1
Ordered Locus Names:MMAR_5138
OrganismMycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]
Taxonomic identifier216594 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length934 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733

Sequence similarities

Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
B2HJ79 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 71154C72C59BECFE

FASTA934102,189
        10         20         30         40         50         60 
MADPKTKGPG SRSSGGNGSG RRLVIVESPT KARKLAGYLG PRYIVESSRG HIRDLPRAAA 

        70         80         90        100        110        120 
DVPAKFKSEP WARLGVNVDA DFEPLYIISP EKKSAVSELK DLLKDVDELY LATDGDREGE 

       130        140        150        160        170        180 
AIAWHLLETL KPRVPVKRMV FHEITEPAIL EAAENPRDLD MDLVDAQETR RILDRLYGYE 

       190        200        210        220        230        240 
VSPVLWKKVA PKLSAGRVQS VATRMIVQRE RDRMAFRSAS YWDIVATLDA SVSDPKAAPP 

       250        260        270        280        290        300 
TFVARLTSVD GLRVATGRDF DSLGTLRKAD EVTVLDEARA AALATGLHGA QLSVASVEEK 

       310        320        330        340        350        360 
PYSRRPYPPF MTSTLQQEAG RKLRFSGERT MSIAQRLYEN GYITYMRTDS TTLSESAINA 

       370        380        390        400        410        420 
ARTQARQLYG EEYVASSPRQ YTRKVKNAQE AHEAIRPAGE TFATPDAVRR ELDGDEFRLY 

       430        440        450        460        470        480 
ELIWQRTVAS QMADARGTTL SLRIGGSAGE QQVVFSASGR TLTFPGFLKA YVETVDELVG 

       490        500        510        520        530        540 
GEADDAERRL PNLTPGQRLD AVELTPDGHT TNPPARYTEA SLVKAFEELG IGRPSTYTPI 

       550        560        570        580        590        600 
IKTIQDRGYV HKKGSALVPS WVAFAVTGLL EQHFGHLVDY GFTAAMEDEL DAIASGQERR 

       610        620        630        640        650        660 
TDWLNNFYFG GDHGVSDSVA RAGGLKKLVG VNLEGIDARE VNSIKLFDDE QGRPIYVRVG 

       670        680        690        700        710        720 
KNGPYLERTI TGEDGEPTPQ RANLNDSLTP DELTLVLAEE LFATPQEGRV LGIDPESGHE 

       730        740        750        760        770        780 
IVAKDGRYGP YVTEILPEPP APEAGTEAPA KKTKKATGPK PRSGSLLRSM DLQTITLEDA 

       790        800        810        820        830        840 
LKLLSLPRVV GTDPASGEEI TAQNGRYGPY LKRGTDSRSL ATEDQIFSIT LDEALKIYAE 

       850        860        870        880        890        900 
PKRSGRQSAS APPLRELGTD AASGKPMVIK DGRFGPYVTD GETNASLRKG DEVASITDER 

       910        920        930 
ASELLADRRA RGPVKRAAKK TTRKSPAKKA VKRA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000854 Genomic DNA. Translation: ACC43542.1.
RefSeqYP_001853397.1. NC_010612.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB2HJ79.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000031692; EBMYCP00000030132; EBMYCG00000031687.
GeneID6229430.
GenomeReviewsGene locus MMAR_5138 in contig CP000854_GR.
KEGGmmi:MMAR_5138.
PATRIC18071693. VBIMycMar75906_5502.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000016895.
HOGENOMHBG512935.
OMASTEPVIG.
ProtClustDBPRK07561.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB2HJ79_MYCMM
AccessionPrimary (citable) accession number: B2HJ79
Entry history
Integrated into UniProtKB/TrEMBL: June 10, 2008
Last sequence update: June 10, 2008
Last modified: December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info