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B2HIL7

- MSL7_MYCMM

UniProt

B2HIL7 - MSL7_MYCMM

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Protein

Phenolphthiocerol synthesis polyketide synthase type I Pks15/1

Gene
pks15/1, msl7, MMAR_1762
Organism
Mycobacterium marinum (strain ATCC BAA-535 / M)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the elongation by iterative transfer of p-hydroxybenzoyl group from FadD22 (pHBA-S-FAdD22) to form p-hydroxyphenylalkanoate (pHPA) intermediates during phenolphthiocerol (PPOL) biosynthesis. PPOL is an important intermediate in the biosynthesis of phenolic glycolipid (mycosid B).1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Cofactori

Binds 1 phosphopantetheine covalently By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei211 – 2111For beta-ketoacyl synthase activity Inferred
Active sitei662 – 6621For acyltransferase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1530 – 154718NADP By similarityAdd
BLAST
Nucleotide bindingi1719 – 173416NADP By similarityAdd
BLAST

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC
  2. cofactor binding Source: InterPro
  3. oxidoreductase activity Source: InterPro
  4. phosphopantetheine binding Source: InterPro
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. Actinobacterium-type cell wall biogenesis Source: UniProtKB
  2. fatty acid biosynthetic process Source: UniProtKB-UniPathway
  3. lipid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BioCyciMMAR216594:GJOB-1772-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenolphthiocerol synthesis polyketide synthase type I Pks15/1
Alternative name(s):
Beta-ketoacyl-acyl-carrier-protein synthase I (EC:2.3.1.41)
Gene namesi
Name:pks15/1
Synonyms:msl7
Ordered Locus Names:MMAR_1762
OrganismiMycobacterium marinum (strain ATCC BAA-535 / M)
Taxonomic identifieri216594 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000001190: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. polyketide synthase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi211 – 2111C → A: The pHBA starter unit is not loaded onto Pks15/1 and thus the pHPA intermediate is not produced. 1 Publication
Mutagenesisi2039 – 20391S → A: The pHBA starter unit is loaded onto Pks15/1, but the pHPA intermediate is not produced. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21042104Phenolphthiocerol synthesis polyketide synthase type I Pks15/1PRO_0000406361Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2039 – 20391O-(pantetheine 4'-phosphoryl)serine Inferred

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi216594.MMAR_1762.

Structurei

3D structure databases

ProteinModelPortaliB2HIL7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2002 – 207675Acyl carrierAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni37 – 466430Beta-ketoacyl synthase By similarityAdd
BLAST
Regioni571 – 887317Acyltransferase By similarityAdd
BLAST
Regioni935 – 1095161Dehydratase By similarityAdd
BLAST
Regioni1400 – 1705306Enoylreductase By similarityAdd
BLAST
Regioni1718 – 1899182Beta-ketoacyl reductase (KR)Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3321.
HOGENOMiHOG000046292.
KOiK12430.
OMAiHAHAVES.
OrthoDBiEOG6W19KW.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 2 hits.
3.90.180.10. 1 hit.
InterProiIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR011032. GroES-like.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020842. PKS/FAS_KR.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR020807. PKS_dehydratase.
IPR020843. PKS_ER.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR015083. Polyketide_synth_docking.
IPR006162. PPantetheine_attach_site.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
PF08990. Docking. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
SMARTiSM00827. PKS_AT. 1 hit.
SM00826. PKS_DH. 1 hit.
SM00829. PKS_ER. 1 hit.
SM00822. PKS_KR. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 1 hit.
[Graphical view]
SUPFAMiSSF101173. SSF101173. 1 hit.
SSF47336. SSF47336. 1 hit.
SSF50129. SSF50129. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 1 hit.
SSF55048. SSF55048. 1 hit.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2HIL7-1 [UniParc]FASTAAdd to Basket

« Hide

MTTSGESADQ QNDKLFRYLK KVAVELDEAR ARLREYEQRA TEPVAVVGIG     50
CRFPGGADGP EGLWDLVSQG RDAVTEFPND RGWDTEGLFD PDPDAEGKTY 100
TRWGAFVENA TNFDAGFFGI PPSEVLAMDP QQRLMLEVSW EALEHAGIDP 150
MSLRGSSTGV FTGIFAPSYG GKDVGALQGY GLTGSPVSVA SGRVAYVLGL 200
EGPALSVDTA CSSSLVAIHW AMASLRSGEC DMALAGGVTV MGLPSIFVGF 250
SRQRGLAADG RCKAFAAAAD GTGWGEGAGV LVLERLSDAQ RNGHNVLAVV 300
RGSAINQDGA SNGLTAPNGL AQQRVIQAAL ANCGLTSADV DVVEAHGTAT 350
TLGDPIEAEA LLATYGQGRP TDQPLWVGSI KSNMGHTQAA AGVAGVIKMV 400
QAMRHGLMPA SLHVDEPSKR VDWESGAVSV LAEARDWPDA GRPRRAGVSS 450
FGISGTNAHV ILEEAPAPEA VPDSESNKGE PSLPVVPWVI SARSAEALTA 500
QAGRLLAHVQ ADPQSNPVDI GFSLAGRSAF EHRAVVVGAD RQQLLTGLAT 550
LADGAPGAGV VTGQAGSVGK TAVVFPGQGS QRIGMARELH DQLPVFAEAF 600
DAVADELDRH LRIPLREVMW GSDAALLDST EFAQPALFAV EVALFAALQR 650
WGLQPDFVMG HSVGELSAAY VAGVLTLADA AMLVVARGRL MQALPAGGAM 700
VAVAAAEDEV LPSLTDGVGI AAINAPKSVV ISGAEAAVTA ISDQFAQQGR 750
RVHRLAVSHA FHSPLMEPML EEFARIAAQV EAREPQIALV SNVTGELASA 800
DGGFGSAQYW VEHVRRAVRF ADSARQLHTL GVTHFVEVGP GSGLTGSIEQ 850
SLAPAEAVVV SMLGKDRPEV ASVLTAFGQL FSTGMSVDWP AVFAGSGATR 900
VDLPTYAFQR RRFWEVPGAD GPADATGLGL GGAEHALLGA VVERPDSGGV 950
VLTGRLALAD QPWLADHVIG GVVLFPGAGF VELAIRAGDE VGCAVVEELV 1000
LAAPLVLHPG MGVQVQVIVG AADDSGNRAL SVYSRGDQSE DWLLNAEGML 1050
GVEAASSGAD LSVWPPEGAE SVDISDGYAQ LADRGYAYGP GFQGLVGVWR 1100
RDSELFAEVV APSGVAVDKM GMHPVVLDAV LHALGLTAEQ NPDSDETKLP 1150
FCWRGVSLHA GGAGRVRARL TMSGPDSISV EIADAAGLPV LTVGALVTRA 1200
MSAAQLRAAV AAAGGGAPDQ GPLDVIWSPI PLSGSGTNGS AQPAVVSWAD 1250
FCAGGDGGAA GDAGVVVWEP NPAGEDVVGS VYAATHAALE VLQSWFDGDR 1300
AGTLVVLTHG AVAMPGENVS DLAGAAVWGI VRSAQAENPG RIVLVDADAA 1350
VEAAELVAVG EPQLVVRSGA AHAARLAPAA PLLAVPADES AWRLAAGGGG 1400
TLEDLVIEPC PEVQAPLAAG QVRVAVRAVG VNFRDVVAAL GMYPGEAPPL 1450
GAEGAGVVLE VGPQVSGVAV GDSVMGFLGG AGPLSVVDQQ LITRMPQGWS 1500
FAQAAAVPVV FLTALFGLQD LAKIQPGESV LIHAGTGGVG MAAVQLARHW 1550
GVEIFVTASR GKWDTLRAMG FDDDHIGDSR TLDFEEKFLA VTDGRGVDVV 1600
LDSLAGDFVD ASLRLLVRGG RFLEMGKTDI RDADKIAANY PGVWYRAFDL 1650
SEAGPVRMQE MLAEVRELFD TAVLHRLPVT TWDVRCAPAA FRFMSQARHI 1700
GKVVLTMPSA LADGLADATV LITGATGAVG AVLARHMLDA YGVRHLVLAS 1750
RRGDRAEGAA ELAAELSEAG ANVQVVACDV ADRDAVEAML ARLSGEYPPV 1800
RGVIHAAGVL DDAVISSLTP ERIDTVLRAK VDAAWNLHEA TLDLDLSMFV 1850
LCSSIAATVG SPGQGNYSAA NSFLDGLAAH RQAAGLAGIS VAWGLWEQSG 1900
GMAAHLSSRD LARMSRSGLA PMNPEQAVGL LDAVLAINHP LMVATLLDRP 1950
ALEARAQAGG LPPLFAGVVR RPRRRQIEDT GDAAQSKSAL AERLNGLSAG 2000
ERQDALVGLV CLQAAAVLGR PSPEDIDPEA GFQDLGFDSL TAVELRNRLK 2050
SATGLTLPPT VIFDHPTPTA IAEYVGRQIP DSQATQAEEE KLPESDGEMV 2100
SVTA 2104
Length:2,104
Mass (Da):217,744
Last modified:June 10, 2008 - v1
Checksum:i7A33F823206BDCA5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000854 Genomic DNA. Translation: ACC40211.1.
RefSeqiWP_012393570.1. NC_010612.1.
YP_001850066.1. NC_010612.1.

Genome annotation databases

EnsemblBacteriaiACC40211; ACC40211; MMAR_1762.
GeneIDi6226021.
KEGGimmi:MMAR_1762.
PATRICi18064397. VBIMycMar75906_1883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000854 Genomic DNA. Translation: ACC40211.1 .
RefSeqi WP_012393570.1. NC_010612.1.
YP_001850066.1. NC_010612.1.

3D structure databases

ProteinModelPortali B2HIL7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216594.MMAR_1762.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACC40211 ; ACC40211 ; MMAR_1762 .
GeneIDi 6226021.
KEGGi mmi:MMAR_1762.
PATRICi 18064397. VBIMycMar75906_1883.

Phylogenomic databases

eggNOGi COG3321.
HOGENOMi HOG000046292.
KOi K12430.
OMAi HAHAVES.
OrthoDBi EOG6W19KW.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci MMAR216594:GJOB-1772-MONOMER.

Family and domain databases

Gene3Di 1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 2 hits.
3.40.50.720. 2 hits.
3.90.180.10. 1 hit.
InterProi IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR011032. GroES-like.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016036. Malonyl_transacylase_ACP-bd.
IPR016040. NAD(P)-bd_dom.
IPR020842. PKS/FAS_KR.
IPR020801. PKS_acyl_transferase.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR020807. PKS_dehydratase.
IPR020843. PKS_ER.
IPR013968. PKS_KR.
IPR020806. PKS_PP-bd.
IPR015083. Polyketide_synth_docking.
IPR006162. PPantetheine_attach_site.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
PF08990. Docking. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view ]
SMARTi SM00827. PKS_AT. 1 hit.
SM00826. PKS_DH. 1 hit.
SM00829. PKS_ER. 1 hit.
SM00822. PKS_KR. 1 hit.
SM00825. PKS_KS. 1 hit.
SM00823. PKS_PP. 1 hit.
[Graphical view ]
SUPFAMi SSF101173. SSF101173. 1 hit.
SSF47336. SSF47336. 1 hit.
SSF50129. SSF50129. 1 hit.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 1 hit.
SSF55048. SSF55048. 1 hit.
PROSITEi PS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-535 / M.
  2. "Cooperation between a coenzyme A-independent stand-alone initiation module and an iterative type I polyketide synthase during synthesis of mycobacterial phenolic glycolipids."
    He W., Soll C.E., Chavadi S.S., Zhang G., Warren J.D., Quadri L.E.
    J. Am. Chem. Soc. 131:16744-16750(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A POLYKETIDE SYNTHASE, MUTAGENESIS OF CYS-211 AND SER-2039.
    Strain: ATCC BAA-535 / M.

Entry informationi

Entry nameiMSL7_MYCMM
AccessioniPrimary (citable) accession number: B2HIL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: June 10, 2008
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi