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B2HIH4

- SYE_MYCMM

UniProt

B2HIH4 - SYE_MYCMM

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Mycobacterium marinum (strain ATCC BAA-535 / M)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei259 – 2591ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMMAR216594:GJOB-1727-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:MMAR_1719
    OrganismiMycobacterium marinum (strain ATCC BAA-535 / M)
    Taxonomic identifieri216594 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000001190: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 489489Glutamate--tRNA ligasePRO_0000367715Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi216594.MMAR_1719.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi12 – 2211"HIGH" regionAdd
    BLAST
    Motifi256 – 2605"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    KOiK01885.
    OMAiPEGMLNY.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B2HIH4-1 [UniParc]FASTAAdd to Basket

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    MTSSSVRVRF CPSPTGIPHV GMVRTALFNW AYARHTGGTF VFRIEDTDAA    50
    RDSEESYLAL LDALRWLGLD WDEGPEVDGP YGPYRQSQRT EIYHDVVAKL 100
    LTAGEAYYAF STPEEVEARH IAAGRNPKLG YDNFDRQLTD SQRAAYLAEG 150
    RKPVVRLRMP DTDLAWHDLV RGPTTFAAGS VPDFALTRAT GDPLYTLVNP 200
    CDDALMKITH VLRGEDLLPS TPRQIALYQA LMRIGIAERV PEFAHLPTVL 250
    GEGTKKLSKR DPQSNLFAHR DRGFIPEGLL NYLALLGWAI ADDHDLFSLD 300
    EMVAAFDVAD VNSNPARFDQ KKADAINAEH IRMLDVADFT ARLRAYLDTH 350
    GHQLALDDAA FAVAAELVQT RIVVLEDAWA LLKFLNDDRY AIDPKAAAKE 400
    LGPDAGPVLD AAIAALDGAA DWTTADIEAA LKTALIEGMA LKPRKAFGPI 450
    RVAATGTTVS PPLFESLELL GRERSLGRLR SARDQVGSP 489
    Length:489
    Mass (Da):53,715
    Last modified:June 10, 2008 - v1
    Checksum:i94C9207AEFE1FC89
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000854 Genomic DNA. Translation: ACC40168.1.
    RefSeqiYP_001850023.1. NC_010612.1.

    Genome annotation databases

    EnsemblBacteriaiACC40168; ACC40168; MMAR_1719.
    GeneIDi6225976.
    KEGGimmi:MMAR_1719.
    PATRICi18064305. VBIMycMar75906_1839.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000854 Genomic DNA. Translation: ACC40168.1 .
    RefSeqi YP_001850023.1. NC_010612.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 216594.MMAR_1719.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACC40168 ; ACC40168 ; MMAR_1719 .
    GeneIDi 6225976.
    KEGGi mmi:MMAR_1719.
    PATRICi 18064305. VBIMycMar75906_1839.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    KOi K01885.
    OMAi PEGMLNY.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci MMAR216594:GJOB-1727-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-535 / M.

    Entry informationi

    Entry nameiSYE_MYCMM
    AccessioniPrimary (citable) accession number: B2HIH4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3