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Protein

Long-chain-fatty-acid--CoA/3-oxocholest-4-en-26-oate--CoA ligase

Gene

fadD19

Organism
Mycobacterium marinum (strain ATCC BAA-535 / M)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the activation of long-chain fatty acids as acyl-coenzyme A (acyl-CoA), which are then transferred to the multifunctional polyketide synthase (PKS) type III for further chain extension. Also involved in the degradation of cholesterol via the degradation of the side chains of C-24 branched-chain sterols. Catalyzes the ATP-dependent CoA thioesterification of the sterol 3-oxocholest-4-en-26-oate to yield 3-oxocholest-4-en-26-oyl-CoA.By similarity

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.By similarity
ATP + (25S)-3-oxocholest-4-en-26-oate + CoA = AMP + diphosphate + (25S)-3-oxocholest-4-en-26-oyl-CoA.By similarity

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Pathwayi: cholesterol metabolism

This protein is involved in the pathway cholesterol metabolism, which is part of Steroid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway cholesterol metabolism and in Steroid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei419ATPBy similarity1
Binding sitei434ATPBy similarity1
Binding sitei525ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 186ATPBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCholesterol metabolism, Fatty acid metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00094
UPA00296

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA/3-oxocholest-4-en-26-oate--CoA ligaseBy similarity (EC:6.2.1.3By similarity, EC:6.2.1.42By similarity)
Short name:
FACLBy similarity
Alternative name(s):
Acyl-CoA synthetaseBy similarity
Steroid-CoA ligaseBy similarity
Steroid-coenzyme A ligaseBy similarity
Gene namesi
Name:fadD19By similarity
Synonyms:fadD19_1
Ordered Locus Names:MMAR_5001
OrganismiMycobacterium marinum (strain ATCC BAA-535 / M)
Taxonomic identifieri216594 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000001190 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004067921 – 550Long-chain-fatty-acid--CoA/3-oxocholest-4-en-26-oate--CoA ligaseAdd BLAST550

Interactioni

Protein-protein interaction databases

STRINGi216594.MMAR_5001

Structurei

3D structure databases

ProteinModelPortaliB2HI05
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG41080W3 Bacteria
ENOG410XNYF LUCA
HOGENOMiHOG000230015
KOiK18688
OMAiYRYVENE
OrthoDBiPOG091H02SZ

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

B2HI05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVALNIADL AEHAIDAVPD RVALICGDEQ LTYAQLEEKA NRLAHHLIDQ
60 70 80 90 100
GVGKGDKVGL YCRNRIEIVI AMLGIIKAGA ILINVNFRYV EGELKYLFDN
110 120 130 140 150
SDMVALVHER QYADRVANVL PDTPNVKTIL VVQDGSDKDY RRYGGVEFYS
160 170 180 190 200
AIADSSPERD FAELQRERSA DDIYILYTGG TTGFPKGVMW RHEDIYRVLF
210 220 230 240 250
GGTDFATGEF IKDEYDLAKA AAANPPMIRY PIPPMIHGAT QSATWMSIFS
260 270 280 290 300
GQTTVLAPEF DADQVWRTIS DRKVNLLFFT GDAMARPLLD ALMKDNDYDL
310 320 330 340 350
SSLFLLASTA ALFSPSIKEK LLELLPNRVI TDSIGSSETG FGGTSIVGAG
360 370 380 390 400
QATTGGPRVT IDHRTVVLDE EGNEVKPGSG VRGIIAKKGN IPVGYYKDEK
410 420 430 440 450
KTAETFKTIN GVRYAIPGDY AMVEADGTVT MLGRGSVSIN SGGEKIYPEE
460 470 480 490 500
VEAALKGHPD VFDALVVGVP DPRYGQHVAA VVAPRPGSRP SLAELDGFVR
510 520 530 540 550
SAIAGYKVPR SLWFVDEVKR SPAGKPDYRW AKEQTEARPA DDVHAAHVSA
Length:550
Mass (Da):59,946
Last modified:June 10, 2008 - v1
Checksum:i6BA317E6E7E31ACB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA Translation: ACC43405.1
RefSeqiWP_012396525.1, NC_010612.1

Genome annotation databases

EnsemblBacteriaiACC43405; ACC43405; MMAR_5001
GeneIDi34340113
KEGGimmi:MMAR_5001

Similar proteinsi

Entry informationi

Entry nameiFAC19_MYCMM
AccessioniPrimary (citable) accession number: B2HI05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: June 10, 2008
Last modified: April 25, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health