B2HH30 (B2HH30_MYCMM) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase PIRNR PIRNR000485
Short name=ATase PIRNR PIRNR000485
EC=2.4.2.14 PIRNR PIRNR000485

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase PIRNR PIRNR000485

Gene names

Name:	purF EMBL ACC43285.1
Ordered Locus Names:	MMAR_4881

Organism

Mycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]

Taxonomic identifier

216594 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O. PIRNR PIRNR000485
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity. PIRSR PIRSR000485-3 Binds 1 magnesium ion per subunit By similarity. PIRNR PIRNR000485
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2. PIRNR PIRNR000485
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. PIRNR PIRNR000485

Ontologies

Keywords
Biological process	Purine biosynthesis PIRNR PIRNR000485
Ligand	Iron Iron-sulfur PIRSR PIRSR000485-3 Magnesium PIRNR PIRNR000485 Metal-binding
Molecular function	Glycosyltransferase PIRNR PIRNR000485 EMBL ACC43285.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	17	1	For GATase activity By similarity PIRSR PIRSR000485-1
	Metal binding	258	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	404	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	460	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3
	Metal binding	463	1	Iron-sulfur (4Fe-4S) By similarity PIRSR PIRSR000485-3

Sequences

	Sequence		Length	Mass (Da)
	B2HH30 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 0B7815943EDA36CF	FASTA	508	54,316
	10 20 30 40 50 60 MTAQQSEEDF NAPREECGVF GVWAPGEDVA KLSYYGLYAL QHRGQEAAGI AVADGSQVLV 70 80 90 100 110 120 FKDLGLVSQV FDEQTLAAMQ GHVAIGHCRY STTGDTTWEN AQPVFRNTAA GTGVALGHNG 130 140 150 160 170 180 NLVNAAALAA RARDEGLIGN RSSAPATTDS DILGALLAHG AADSSLEQAA LELLPTVRGA 190 200 210 220 230 240 FCLTFMDENT LYACRDPHGV RPLSLGRLDR GWVVASETAA LDIVGASFVR DIEPGELLAI 250 260 270 280 290 300 DADGVRSTRF ANPTPKGCVF EYVYLARPDS MLNGRSVHAA RVAIGRRLAQ ERRVEADLVI 310 320 330 340 350 360 GVPESGTPAA VGYAQESGIP YGQGLMKNAY VGRTFIQPSQ TIRQLGIRLK LNPLKEVIRG 370 380 390 400 410 420 KRLIVVDDSI VRGNTQRALV RMLREAGAVE VHVRIASPPV KWPCFYGIDF PSPAELIANA 430 440 450 460 470 480 VEDENEMLEA VRHVINADTL GYISLRGLVA ATEQPISRLC TACFDGKYPI ELPGETALGK 490 500 NVIEHMLANA ARGAELGDVA TDEVPIGH

References

[1]

"Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis." Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K., Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C., Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K., Lord A., Moule S. , Mungall K., Norbertczak H., Quail M.A., Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R., Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T. Genome Res. 18:729-741(2008) [PubMed: 18403782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000854 Genomic DNA. Translation: ACC43285.1.
RefSeq	YP_001853140.1. NC_010612.1.
3D structure databases
ProteinModelPortal	B2HH30.
ModBase	Search...
Protein-protein interaction databases
STRING	B2HH30.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000034487; EBMYCP00000032927; EBMYCG00000034482.
GeneID	6229171.
GenomeReviews	Gene locus MMAR_4881 in contig CP000854_GR.
KEGG	mmi:MMAR_4881.
PATRIC	18071151. VBIMycMar75906_5233.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016850.
HOGENOM	HBG392416.
OMA	VWAPGEE.
ProtClustDB	PRK07847.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR000583. GATase_2. IPR017932. GATase_II. IPR000836. PRibTrfase. [Graphical view]
KO	K00764.
PANTHER	PTHR11907. Amd_phspho_trans. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. PurF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B2HH30_MYCMM

Accession

Primary (citable) accession number: B2HH30

Entry history

Integrated into UniProtKB/TrEMBL:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)