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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Mycobacterium marinum (strain ATCC BAA-535 / M)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi283 – 2831Manganese 2UniRule annotation
Metal bindingi288 – 2881Manganese 1UniRule annotation
Metal bindingi288 – 2881Manganese 2UniRule annotation
Active sitei295 – 2951UniRule annotation
Metal bindingi306 – 3061Manganese 2UniRule annotation
Metal bindingi365 – 3651Manganese 1UniRule annotation
Metal bindingi367 – 3671Manganese 1UniRule annotation
Metal bindingi367 – 3671Manganese 2UniRule annotation
Active sitei369 – 3691UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMMAR216594:GJOB-3277-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:MMAR_3258
OrganismiMycobacterium marinum (strain ATCC BAA-535 / M)
Taxonomic identifieri216594 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000001190 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Probable cytosol aminopeptidasePRO_1000098331Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216594.MMAR_3258.

Structurei

3D structure databases

ProteinModelPortaliB2HGY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243129.
KOiK01255.
OMAiVQWAHID.
OrthoDBiPOG091H01BT.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2HGY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAESGDQIP TVHVATSLPK RGVSSSVLIV PVVSTGDDEQ SGERPGAVVA
60 70 80 90 100
SAEPFLSADA IAEIEAGLRA LDATGASDQV HRLVVSSLPV SSVLTVGLGK
110 120 130 140 150
PRYEWTPDAV RRAAGAAARA LGTAKTVVTT LADLPGDGVC AAAIEGLILG
160 170 180 190 200
SYRFSAFRSA KTAPKDAGLH KVTVLTTAKD ARKHCAHGAA VATAVATARD
210 220 230 240 250
LVNTPPSHLY PAELARRAKV LGESVGLEVQ VLDEKALQKA GYGGLVGVGQ
260 270 280 290 300
GSSRPPRLVR LTHRGSRLAK NPRRAKKVAL VGKGVTFDTG GISIKPAASM
310 320 330 340 350
HYMTSDMGGA AAVIATVALA AQLELPIDVI ATVPIAENMP SATAQRPGDV
360 370 380 390 400
LTQYGGTTVE VLNTDAEGRL ILADAIVRAC EDNPDYLIET STLTGAQTVA
410 420 430 440 450
LGARIPGVMG SDEFRDRVAA ISQQVGENGW PMPLPDELKD DLKSTVADLA
460 470 480 490 500
NVSGQRFAGM LVAGVFLREF VADAVDWAHI DVAGPAYNTG SAWGYTPKGA
510
TGVPTRTMFA VLEDIAANG
Length:519
Mass (Da):53,539
Last modified:June 10, 2008 - v1
Checksum:i58BFEDF9C0C40AD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA. Translation: ACC41685.1.
RefSeqiWP_012394915.1. NC_010612.1.

Genome annotation databases

EnsemblBacteriaiACC41685; ACC41685; MMAR_3258.
KEGGimmi:MMAR_3258.
PATRICi18067615. VBIMycMar75906_3487.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA. Translation: ACC41685.1.
RefSeqiWP_012394915.1. NC_010612.1.

3D structure databases

ProteinModelPortaliB2HGY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216594.MMAR_3258.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC41685; ACC41685; MMAR_3258.
KEGGimmi:MMAR_3258.
PATRICi18067615. VBIMycMar75906_3487.

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243129.
KOiK01255.
OMAiVQWAHID.
OrthoDBiPOG091H01BT.

Enzyme and pathway databases

BioCyciMMAR216594:GJOB-3277-MONOMER.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPA_MYCMM
AccessioniPrimary (citable) accession number: B2HGY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: September 7, 2016
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.