B2HGL4 (GLSA_MYCMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase
EC=3.5.1.2

Gene names

Name:	glsA
Ordered Locus Names:	MMAR_1548

Organism

Mycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]

Taxonomic identifier

216594 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium marinum

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glutaminase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutaminase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Glutaminase HAMAP-Rule MF_00313		PRO_1000115699
Sites
	Binding site	70	1	Substrate By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	165	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	248	1	Substrate By similarity
	Binding site	266	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2HGL4 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 538A7317BF2254ED	FASTA	320	33,748
	10 20 30 40 50 60 MAPTSVSNAR IEKAVLDAHE KQKNKHGGKN ADYIPILAQV PSTLFGVSVA TVDGQVFTAG 70 80 90 100 110 120 DAGYEFALES ISKIFTLALV IEQRGPRELR LKVGADPTGE AFNSVLALEL HNDKPMSPLV 130 140 150 160 170 180 NAGAISTTSL VDAVGPEDRW RQIVGAQSDF AGRQISISEE INASEQATNF HNRAIAWLLR 190 200 210 220 230 240 GSGYIYCDPM EACDIYTRQC STLVTTADLA VMGATLANGG TNPITGKRVI ARKNVPHVLA 250 260 270 280 290 300 EMTMEGVYTR SGDWAYTVGL PAKSGVGGGL VAVAPGQLAI AAFSPPLDKV GNSVRAQAAV 310 320 AQIADTLQLG LFNVPGEEDE

References

[1]

"Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis." Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K., Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C., Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K., Lord A., Moule S. , Mungall K., Norbertczak H., Quail M.A., Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R., Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T. Genome Res. 18:729-741(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-535 / M.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000854 Genomic DNA. Translation: ACC40000.1.
RefSeq	YP_001849855.1. NC_010612.1.
3D structure databases
ProteinModelPortal	B2HGL4.
SMR	B2HGL4. Positions 10-312.
ModBase	Search...
Protein-protein interaction databases
STRING	216594.MMAR_1548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACC40000; ACC40000; MMAR_1548.
GeneID	6225805.
KEGG	mmi:MMAR_1548.
PATRIC	18063921. VBIMycMar75906_1648.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
OMA	NARADES.
ProtClustDB	PRK12356.
Enzyme and pathway databases
BioCyc	MMAR216594:GJOB-1556-MONOMER.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA_MYCMM

Accession

Primary (citable) accession number: B2HGL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program