B2HES1 (AROA_MYCMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	MMAR_1320

Organism

Mycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]

Taxonomic identifier

216594 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000099724

Sequences

	Sequence		Length	Mass (Da)
	B2HES1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 06CF21673420C4FC	FASTA	431	44,617
	10 20 30 40 50 60 MSSIEPWPAP FAPTPVHATV TVPGSKSQTN RTLVLAALAA AQGQGSSTIT GALRSRDTDL 70 80 90 100 110 120 MIEALQTLGL RVDGTGSELT VSGRIRPGPE ARVDCGLAGT VLRFVPPLAA LSAAPITFDG 130 140 150 160 170 180 DEQARARPIA PLLDALRGLG VPVDGAGLPF RVQGTGSVAG GTVAIDASAS SQFVSGLLLS 190 200 210 220 230 240 GASFTDGLTV QHTGSELPSA PHIAMTVQML RQAGVDVDDS IPNRWLVRPG ALRPRHWDVE 250 260 270 280 290 300 PDLTNAVAFL AAAVVTGGTV TITGWPADSV QPAKNILDIL QTLNSTVRHI DSCLQVQGPQ 310 320 330 340 350 360 TYRGFDVDLR DVGELTPSVA ALAALASPGS VSRLAGIAHL RGHETDRLAA LSTEINRLGG 370 380 390 400 410 420 NCEQTSDGLV ITATPLRPGS WRAYADHRMA MAGAIVGLRV AGVEVDDIGA TSKTLPEFPQ 430 LWTEMVEGSS G

References

[1]

"Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis." Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K., Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C., Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K., Lord A., Moule S. , Mungall K., Norbertczak H., Quail M.A., Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R., Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T. Genome Res. 18:729-741(2008) [PubMed: 18403782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-535 / M.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000854 Genomic DNA. Translation: ACC39778.1.
RefSeq	YP_001849633.1. NC_010612.1.
3D structure databases
ProteinModelPortal	B2HES1.
ModBase	Search...
Protein-protein interaction databases
STRING	B2HES1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000035963; EBMYCP00000034403; EBMYCG00000035958.
GeneID	6225575.
GenomeReviews	Gene locus MMAR_1320 in contig CP000854_GR.
KEGG	mmi:MMAR_1320.
PATRIC	18063431. VBIMycMar75906_1404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016231.
HOGENOM	HBG646626.
OMA	HRMATAG.
ProtClustDB	PRK02427.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_MYCMM

Accession

Primary (citable) accession number: B2HES1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program