B2HE73 (KATG_MYCMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	MMAR_2914

Organism

Mycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]

Taxonomic identifier

216594 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Catalase-peroxidase HAMAP MF_01961		PRO_0000354838
Sites
	Active site	111	1	Proton acceptor By similarity
	Metal binding	274	1	Iron (heme axial ligand) By similarity
	Site	107	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	110 ↔ 233		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-259) By similarity
	Cross-link	233 ↔ 259		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-110) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2HE73 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: EB005AD6563C791E	FASTA	743	81,032
	10 20 30 40 50 60 MSSDSRPPQP DTSTQSNSES ESPAISSPTP QDHAPMTNRD WWPNQIDVSM LHPHPSQASP 70 80 90 100 110 120 LGADFDYPKE FAKLDVDALK ADVMSVMTTS QDWWPADYGH YGGLFIRMSW HAAGTYRIQD 130 140 150 160 170 180 GRGGGGQGMQ RFAPLNSWPD NVSLDKARRL LWPVKQKYGS KISWADLIIF AGNCALDSMG 190 200 210 220 230 240 FKTFGFGFGR EDVWQPEEVM WGEEDVWLGT DKRYSGKRDL AQPYGATTMG LIYVNPEGPE 250 260 270 280 290 300 GKPDPVAAAH DIRETFARMA MNDEETAALI VGGHSFGKTH GAGDADLVGP EPEAAPIEQQ 310 320 330 340 350 360 GFGWKSSFGS GKGKDAITSG LEVVWTPTPT QWGNGFLELL YGYEWELTKS PAGAWQFTAK 370 380 390 400 410 420 DGAGAGTIPD PFGGPGRAPT MLVTDISMRE DPIYRRITQR WLEHPEELTE AFAKAWYKLL 430 440 450 460 470 480 HRDMGPVSRY LGPWVAEPQL WQDPVPDVDH ELVDAKDVAA LKSKVLASGL TVAQLVKTAW 490 500 510 520 530 540 SAASSFRRTD KRGGANGGRL RLEPQKSWES NEPADLDQVL SVLEGIQQDF NSSAAGGKKI 550 560 570 580 590 600 SLADLIVLAG SAAVEKAAKD GGHEVSVPFA PGRTDASQEN TDVESFAVLE PRADGFRNYV 610 620 630 640 650 660 RVGEKAPLEH LLIERAYRLG VTAPEMTVLV GGLRALGANH GGSKHGVFTD NPGVLSNDFF 670 680 690 700 710 720 VNLLDMGTEW KASEAAENVY EGCDRSSGQL KWTATANDLV FGSNSVLRAL AEVYAQSDAK 730 740 QKFAEDFAAA WAKVMNNDRF DLE

References

[1]

"Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis." Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K., Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C., Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K., Lord A., Moule S. , Mungall K., Norbertczak H., Quail M.A., Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R., Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T. Genome Res. 18:729-741(2008) [PubMed: 18403782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-535 / M.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000854 Genomic DNA. Translation: ACC41353.1.
RefSeq	YP_001851208.1. NC_010612.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2HE73.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000034145; EBMYCP00000032585; EBMYCG00000034140.
GeneID	6227181.
GenomeReviews	Gene locus MMAR_2914 in contig CP000854_GR.
KEGG	mmi:MMAR_2914.
PATRIC	18066865. VBIMycMar75906_3112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017549.
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MYCMM

Accession

Primary (citable) accession number: B2HE73

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program