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B2GV87 (PTPRE_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Receptor-type tyrosine-protein phosphatase epsilon

Short name=Protein-tyrosine phosphatase epsilon
Short name=R-PTP-epsilon
EC=3.1.3.48
Gene names
Name:Ptpre
Synonyms:Ptpe
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length699 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function By similarity. Acts as a negative regulator of insulin receptor (IR) signaling and is involved in insulin-induced glucose metabolism mainly through direct dephosphorylation and inactivation of IR in hepatocytes and liver. Ref.6

Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake By similarity. Ref.6

Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca2+ By similarity. Ref.6

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Monomer. Isoform 2:Homodimer. Can form oligomers. Dimerization is increased by oxidative stress and decreased by EGFR. Isoform 2 interacts with GRB2 By similarity.

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein By similarity.

Isoform 2: Cytoplasm By similarity. Note: Predominantly cytoplasmic. A small fraction is also associated with nucleus and membrane. Insulin can induce translocation to the membrane By similarity.

Isoform 3: Cytoplasm By similarity.

Tissue specificity

Isoform 1 is highly expressed in the brain, lung, spleen and testis. Isoform 2 is highly expressed in thymus, spleen and lung. Isoform 1 and isoform 2 are expressed in primary hepatocytes. Ref.2 Ref.6

Domain

The tyrosine-protein phosphatase 2 domain (D2) mediates dimerization. The extreme N- and C- termini of the D2 domain act to inhibit dimerization and removal of these sequences increases dimerization and inhibits enzyme activity By similarity.

Post-translational modification

A catalytically active cytoplasmic form (p65) is produced by proteolytic cleavage of either isoform 1, isoform 2 or isoform 3 By similarity.

Isoform 1 and isoform 2 are phosphorylated on tyrosine residues by tyrosine kinase Neu By similarity.

N-glycosylated By similarity.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Receptor class 4 subfamily.

Contains 2 tyrosine-protein phosphatase domains.

Sequence caution

The sequence AAI66573.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 3 isoforms produced by alternative promoter usage and alternative initiation. [Align] [Select]
Isoform 1 (identifier: B2GV87-1)

Also known as: PTPeM; RPTPe; tm-PTPe;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by alternative promoter usage.
Isoform 2 (identifier: B2GV87-2)

Also known as: PTPeC; cyt-PTPe;

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: MEPFCPLLLA...LFLLAAYFFR → MSSRKNFSRLTW
Note: Produced by alternative promoter usage.
Isoform 3 (identifier: B2GV87-3)

Also known as: p67;

The sequence of this isoform differs from the canonical sequence as follows:
     1-84: Missing.
Note: Produced by alternative initiation at Met-85 of isoform 1.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 699677Receptor-type tyrosine-protein phosphatase epsilon
PRO_0000389638

Regions

Topological domain23 – 4725Extracellular Potential
Transmembrane48 – 6821Helical; Potential
Topological domain69 – 699631Cytoplasmic Potential
Domain134 – 393260Tyrosine-protein phosphatase 1
Domain425 – 688264Tyrosine-protein phosphatase 2
Region334 – 3407Substrate binding By similarity
Compositional bias52 – 6312Poly-Leu

Sites

Active site3341Phosphocysteine intermediate By similarity
Active site6291Phosphocysteine intermediate By similarity
Binding site3021Substrate By similarity
Binding site3781Substrate By similarity

Amino acid modifications

Modified residue6951Phosphotyrosine By similarity
Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation311N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 8484Missing in isoform 3.
VSP_038488
Alternative sequence1 – 6969MEPFC…AYFFR → MSSRKNFSRLTW in isoform 2.
VSP_038489

Experimental info

Sequence conflict41F → L in BAA11433. Ref.1
Sequence conflict5731K → T in BAA20333. Ref.1
Sequence conflict5931G → A in BAA20333. Ref.1
Sequence conflict610 – 6112IA → LS in BAA20333. Ref.1
Sequence conflict6211G → D in BAA20333. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (PTPeM) (RPTPe) (tm-PTPe) [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 799F63B840FF7C67

FASTA69980,734
        10         20         30         40         50         60 
MEPFCPLLLA SFSLSLATAG QGNDTTPTES NWTSTTAGPP DPGTSQPLLT WLLLPLLLLL 

        70         80         90        100        110        120 
FLLAAYFFRF RKQRKAVVNS NDKKMPNGIL EEQEQQRVML LSRSPSGPKK YFPIPVEHLE 

       130        140        150        160        170        180 
EEIRVRSADD CKRFREEFNS LPSGHIQGTF ELANKEENRE KNRYPNILPN DHCRVILSQL 

       190        200        210        220        230        240 
DGIPCSDYIN ASYIDGYKEK NKFIAAQGPK QETVNDFWRM VWEQRSATIV MLTNLKERKE 

       250        260        270        280        290        300 
EKCYQYWPDQ GCWTYGNIRV CVEDCVVLVD YTIRKFCIHP QLPDSCKAPR LVSQLHFTSW 

       310        320        330        340        350        360 
PDFGVPFTPI GMLKFLKKVK TLNPSHAGPI VVHCSAGVGR TGTFIVIDAM MDMIHSEQKV 

       370        380        390        400        410        420 
DVFEFVSRIR NQRPQMVQTD VQYTFIYQAL LEYYLYGDTE LDVSSLERHL QTLHGTATHF 

       430        440        450        460        470        480 
DKIGLEEEFR KLTNVRIMKE NMRTGNLPAN MKKARVIQII PYDFNRVILS MKRGQEFTDY 

       490        500        510        520        530        540 
INASFIDGYR QKDYFMATQG PLAHTVEDFW RMVWEWKSHT IVMLTEVQER EQDKCYQYWP 

       550        560        570        580        590        600 
TEGSVTHGDI TIEIKSDTLS EAISIRDFLV TFKQPLARQE EQVRMVRQFH FHGWPEVGIP 

       610        620        630        640        650        660 
TEGKGMIDLI AAVQKQQQQT GNHPITVHCS AGAGRTGTFI ALSNILERVK AEGLLDVFQA 

       670        680        690 
VKSLRLQRPH MVQTLEQYEF CYKVVQDFID IFSDYANFK 

« Hide

Isoform 2 (PTPeC) (cyt-PTPe) [UniParc].

Checksum: 8D2C47A09BAFE304
Show »

FASTA64274,806
Isoform 3 (p67) [UniParc].

Checksum: D258D7FD1F75638E
Show »

FASTA61571,511

References

« Hide 'large scale' references
[1]"Molecular cloning of a novel cytoplasmic protein tyrosine phosphatase PTP epsilon."
Nakamura K., Mizuno Y., Kikuchi K.
Biochem. Biophys. Res. Commun. 218:726-732(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-297 (ISOFORM 1).
Tissue: Spleen.
[2]"Distinct promoters control transmembrane and cytosolic protein tyrosine phosphatase epsilon expression during macrophage differentiation."
Tanuma N., Nakamura K., Kikuchi K.
Eur. J. Biochem. 259:46-54(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE PROMOTER USAGE, TISSUE SPECIFICITY.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[5]Strausberg R.L.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-131 (ISOFORM 3).
Tissue: Placenta.
[6]"Receptor-type protein tyrosine phosphatase epsilon (PTPepsilonM) is a negative regulator of insulin signaling in primary hepatocytes and liver."
Nakagawa Y., Aoki N., Aoyama K., Shimizu H., Shimano H., Yamada N., Miyazaki H.
Zool. Sci. 22:169-175(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION (ISOFORM 1), TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D78610 mRNA. Translation: BAA20333.1.
D78613 mRNA. Translation: BAA11433.1.
CH473953 Genomic DNA. Translation: EDM11793.1.
CH473953 Genomic DNA. Translation: EDM11794.1.
BC166573 mRNA. Translation: AAI66573.1. Different initiation.
CV110063 mRNA. No translation available.
RefSeqNP_446219.1. NM_053767.1.
XP_006230511.1. XM_006230449.1.
UniGeneRn.107819.

3D structure databases

ProteinModelPortalB2GV87.
SMRB2GV87. Positions 110-690.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActB2GV87. 1 interaction.

PTM databases

PhosphoSiteB2GV87.

Proteomic databases

PaxDbB2GV87.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000021359; ENSRNOP00000021359; ENSRNOG00000015717. [B2GV87-1]
ENSRNOT00000021379; ENSRNOP00000021379; ENSRNOG00000015717. [B2GV87-2]
GeneID114767.
KEGGrno:114767.
UCSCRGD:620771. rat. [B2GV87-1]

Organism-specific databases

CTD5791.
RGD620771. Ptpre.

Phylogenomic databases

eggNOGCOG5599.
GeneTreeENSGT00590000082937.
HOGENOMHOG000231465.
HOVERGENHBG053758.
InParanoidQ63476.
KOK18033.
OMAPDGCKAP.
OrthoDBEOG7B31M8.
PhylomeDBB2GV87.

Gene expression databases

GenevestigatorB2GV87.

Family and domain databases

InterProIPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016336. Tyr_Pase_rcpt_a/e-type.
[Graphical view]
PfamPF00102. Y_phosphatase. 2 hits.
[Graphical view]
PIRSFPIRSF002006. PTPR_alpha_epsilon. 1 hit.
PRINTSPR00700. PRTYPHPHTASE.
SMARTSM00194. PTPc. 2 hits.
[Graphical view]
PROSITEPS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio618861.
PROB2GV87.

Entry information

Entry namePTPRE_RAT
AccessionPrimary (citable) accession number: B2GV87
Secondary accession number(s): Q63476, Q63477
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: June 10, 2008
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families