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B2GV87

- PTPRE_RAT

UniProt

B2GV87 - PTPRE_RAT

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Protein

Receptor-type tyrosine-protein phosphatase epsilon

Gene

Ptpre

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function (By similarity). Acts as a negative regulator of insulin receptor (IR) signaling and is involved in insulin-induced glucose metabolism mainly through direct dephosphorylation and inactivation of IR in hepatocytes and liver.By similarity
Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake (By similarity).By similarity
Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca2+.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei302 – 3021SubstrateBy similarity
Active sitei334 – 3341Phosphocysteine intermediateBy similarity
Binding sitei378 – 3781SubstrateBy similarity
Active sitei629 – 6291Phosphocysteine intermediateBy similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: RGD

GO - Biological processi

  1. negative regulation of insulin receptor signaling pathway Source: UniProtKB
  2. peptidyl-tyrosine dephosphorylation Source: GOC
  3. regulation of mast cell activation Source: Ensembl
  4. transmembrane receptor protein tyrosine phosphatase signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase epsilon (EC:3.1.3.48)
Short name:
Protein-tyrosine phosphatase epsilon
Short name:
R-PTP-epsilon
Gene namesi
Name:Ptpre
Synonyms:Ptpe
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi620771. Ptpre.

Subcellular locationi

Isoform 2 : Cytoplasm By similarity
Note: Predominantly cytoplasmic. A small fraction is also associated with nucleus and membrane. Insulin can induce translocation to the membrane (By similarity).By similarity
Isoform 3 : Cytoplasm By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 4725ExtracellularSequence AnalysisAdd
BLAST
Transmembranei48 – 6821HelicalSequence AnalysisAdd
BLAST
Topological domaini69 – 699631CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. nucleus Source: Ensembl
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 699677Receptor-type tyrosine-protein phosphatase epsilonPRO_0000389638Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi31 – 311N-linked (GlcNAc...)Sequence Analysis
Modified residuei695 – 6951PhosphotyrosineBy similarity

Post-translational modificationi

A catalytically active cytoplasmic form (p65) is produced by proteolytic cleavage of either isoform 1, isoform 2 or isoform 3.By similarity
Isoform 1 and isoform 2 are phosphorylated on tyrosine residues by tyrosine kinase Neu.By similarity
N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiB2GV87.

PTM databases

PhosphoSiteiB2GV87.

Expressioni

Tissue specificityi

Isoform 1 is highly expressed in the brain, lung, spleen and testis. Isoform 2 is highly expressed in thymus, spleen and lung. Isoform 1 and isoform 2 are expressed in primary hepatocytes.2 Publications

Gene expression databases

ExpressionAtlasiB2GV87. baseline.
GenevestigatoriB2GV87.

Interactioni

Subunit structurei

Monomer. Isoform 2: Homodimer. Can form oligomers. Dimerization is increased by oxidative stress and decreased by EGFR. Isoform 2 interacts with GRB2 (By similarity).By similarity

Protein-protein interaction databases

IntActiB2GV87. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliB2GV87.
SMRiB2GV87. Positions 110-690.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini134 – 393260Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd
BLAST
Domaini425 – 688264Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni334 – 3407Substrate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi52 – 6312Poly-LeuAdd
BLAST

Domaini

The tyrosine-protein phosphatase 2 domain (D2) mediates dimerization. The extreme N- and C- termini of the D2 domain act to inhibit dimerization and removal of these sequences increases dimerization and inhibits enzyme activity (By similarity).By similarity

Sequence similaritiesi

Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000231465.
HOVERGENiHBG053758.
InParanoidiB2GV87.
KOiK18033.
OMAiIVIDAMI.
OrthoDBiEOG7B31M8.
PhylomeDBiB2GV87.

Family and domain databases

Gene3Di3.90.190.10. 2 hits.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016336. Tyr_Pase_rcpt_a/e-type.
[Graphical view]
PfamiPF00102. Y_phosphatase. 2 hits.
[Graphical view]
PIRSFiPIRSF002006. PTPR_alpha_epsilon. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative initiation. Align

Isoform 1 (identifier: B2GV87-1) [UniParc]FASTAAdd to Basket

Also known as: PTPeM, RPTPe, tm-PTPe

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPFCPLLLA SFSLSLATAG QGNDTTPTES NWTSTTAGPP DPGTSQPLLT
60 70 80 90 100
WLLLPLLLLL FLLAAYFFRF RKQRKAVVNS NDKKMPNGIL EEQEQQRVML
110 120 130 140 150
LSRSPSGPKK YFPIPVEHLE EEIRVRSADD CKRFREEFNS LPSGHIQGTF
160 170 180 190 200
ELANKEENRE KNRYPNILPN DHCRVILSQL DGIPCSDYIN ASYIDGYKEK
210 220 230 240 250
NKFIAAQGPK QETVNDFWRM VWEQRSATIV MLTNLKERKE EKCYQYWPDQ
260 270 280 290 300
GCWTYGNIRV CVEDCVVLVD YTIRKFCIHP QLPDSCKAPR LVSQLHFTSW
310 320 330 340 350
PDFGVPFTPI GMLKFLKKVK TLNPSHAGPI VVHCSAGVGR TGTFIVIDAM
360 370 380 390 400
MDMIHSEQKV DVFEFVSRIR NQRPQMVQTD VQYTFIYQAL LEYYLYGDTE
410 420 430 440 450
LDVSSLERHL QTLHGTATHF DKIGLEEEFR KLTNVRIMKE NMRTGNLPAN
460 470 480 490 500
MKKARVIQII PYDFNRVILS MKRGQEFTDY INASFIDGYR QKDYFMATQG
510 520 530 540 550
PLAHTVEDFW RMVWEWKSHT IVMLTEVQER EQDKCYQYWP TEGSVTHGDI
560 570 580 590 600
TIEIKSDTLS EAISIRDFLV TFKQPLARQE EQVRMVRQFH FHGWPEVGIP
610 620 630 640 650
TEGKGMIDLI AAVQKQQQQT GNHPITVHCS AGAGRTGTFI ALSNILERVK
660 670 680 690
AEGLLDVFQA VKSLRLQRPH MVQTLEQYEF CYKVVQDFID IFSDYANFK

Note: Produced by alternative promoter usage.

Length:699
Mass (Da):80,734
Last modified:June 10, 2008 - v1
Checksum:i799F63B840FF7C67
GO
Isoform 2 (identifier: B2GV87-2) [UniParc]FASTAAdd to Basket

Also known as: PTPeC, cyt-PTPe

The sequence of this isoform differs from the canonical sequence as follows:
     1-69: MEPFCPLLLA...LFLLAAYFFR → MSSRKNFSRLTW

Note: Produced by alternative promoter usage.

Show »
Length:642
Mass (Da):74,806
Checksum:i8D2C47A09BAFE304
GO
Isoform 3 (identifier: B2GV87-3) [UniParc]FASTAAdd to Basket

Also known as: p67

The sequence of this isoform differs from the canonical sequence as follows:
     1-84: Missing.

Note: Produced by alternative initiation at Met-85 of isoform 1.

Show »
Length:615
Mass (Da):71,511
Checksum:iD258D7FD1F75638E
GO

Sequence cautioni

The sequence AAI66573.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41F → L in BAA11433. (PubMed:8579581)Curated
Sequence conflicti573 – 5731K → T in BAA20333. (PubMed:8579581)Curated
Sequence conflicti593 – 5931G → A in BAA20333. (PubMed:8579581)Curated
Sequence conflicti610 – 6112IA → LS in BAA20333. (PubMed:8579581)Curated
Sequence conflicti621 – 6211G → D in BAA20333. (PubMed:8579581)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8484Missing in isoform 3. 1 PublicationVSP_038488Add
BLAST
Alternative sequencei1 – 6969MEPFC…AYFFR → MSSRKNFSRLTW in isoform 2. 1 PublicationVSP_038489Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78610 mRNA. Translation: BAA20333.1.
D78613 mRNA. Translation: BAA11433.1.
CH473953 Genomic DNA. Translation: EDM11793.1.
CH473953 Genomic DNA. Translation: EDM11794.1.
BC166573 mRNA. Translation: AAI66573.1. Different initiation.
CV110063 mRNA. No translation available.
RefSeqiNP_446219.1. NM_053767.1. [B2GV87-1]
XP_006230511.1. XM_006230449.2. [B2GV87-1]
XP_008758193.1. XM_008759971.1. [B2GV87-1]
XP_008758194.1. XM_008759972.1. [B2GV87-1]
UniGeneiRn.107819.

Genome annotation databases

EnsembliENSRNOT00000021359; ENSRNOP00000021359; ENSRNOG00000015717. [B2GV87-1]
ENSRNOT00000021379; ENSRNOP00000021379; ENSRNOG00000015717. [B2GV87-2]
GeneIDi114767.
KEGGirno:114767.
UCSCiRGD:620771. rat. [B2GV87-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D78610 mRNA. Translation: BAA20333.1 .
D78613 mRNA. Translation: BAA11433.1 .
CH473953 Genomic DNA. Translation: EDM11793.1 .
CH473953 Genomic DNA. Translation: EDM11794.1 .
BC166573 mRNA. Translation: AAI66573.1 . Different initiation.
CV110063 mRNA. No translation available.
RefSeqi NP_446219.1. NM_053767.1. [B2GV87-1 ]
XP_006230511.1. XM_006230449.2. [B2GV87-1 ]
XP_008758193.1. XM_008759971.1. [B2GV87-1 ]
XP_008758194.1. XM_008759972.1. [B2GV87-1 ]
UniGenei Rn.107819.

3D structure databases

ProteinModelPortali B2GV87.
SMRi B2GV87. Positions 110-690.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi B2GV87. 1 interaction.

PTM databases

PhosphoSitei B2GV87.

Proteomic databases

PaxDbi B2GV87.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000021359 ; ENSRNOP00000021359 ; ENSRNOG00000015717 . [B2GV87-1 ]
ENSRNOT00000021379 ; ENSRNOP00000021379 ; ENSRNOG00000015717 . [B2GV87-2 ]
GeneIDi 114767.
KEGGi rno:114767.
UCSCi RGD:620771. rat. [B2GV87-1 ]

Organism-specific databases

CTDi 5791.
RGDi 620771. Ptpre.

Phylogenomic databases

eggNOGi COG5599.
GeneTreei ENSGT00760000118900.
HOGENOMi HOG000231465.
HOVERGENi HBG053758.
InParanoidi B2GV87.
KOi K18033.
OMAi IVIDAMI.
OrthoDBi EOG7B31M8.
PhylomeDBi B2GV87.

Miscellaneous databases

NextBioi 618861.
PROi B2GV87.

Gene expression databases

ExpressionAtlasi B2GV87. baseline.
Genevestigatori B2GV87.

Family and domain databases

Gene3Di 3.90.190.10. 2 hits.
InterProi IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR016336. Tyr_Pase_rcpt_a/e-type.
[Graphical view ]
Pfami PF00102. Y_phosphatase. 2 hits.
[Graphical view ]
PIRSFi PIRSF002006. PTPR_alpha_epsilon. 1 hit.
PRINTSi PR00700. PRTYPHPHTASE.
SMARTi SM00194. PTPc. 2 hits.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 2 hits.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a novel cytoplasmic protein tyrosine phosphatase PTP epsilon."
    Nakamura K., Mizuno Y., Kikuchi K.
    Biochem. Biophys. Res. Commun. 218:726-732(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-297 (ISOFORM 1).
    Tissue: Spleen.
  2. "Distinct promoters control transmembrane and cytosolic protein tyrosine phosphatase epsilon expression during macrophage differentiation."
    Tanuma N., Nakamura K., Kikuchi K.
    Eur. J. Biochem. 259:46-54(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE PROMOTER USAGE, TISSUE SPECIFICITY.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. Strausberg R.L.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-131 (ISOFORM 3).
    Tissue: Placenta.
  6. "Receptor-type protein tyrosine phosphatase epsilon (PTPepsilonM) is a negative regulator of insulin signaling in primary hepatocytes and liver."
    Nakagawa Y., Aoki N., Aoyama K., Shimizu H., Shimano H., Yamada N., Miyazaki H.
    Zool. Sci. 22:169-175(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION (ISOFORM 1), TISSUE SPECIFICITY.

Entry informationi

Entry nameiPTPRE_RAT
AccessioniPrimary (citable) accession number: B2GV87
Secondary accession number(s): Q63476, Q63477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: June 10, 2008
Last modified: November 26, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3