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B2GV87

- PTPRE_RAT

UniProt

B2GV87 - PTPRE_RAT

Protein

Receptor-type tyrosine-protein phosphatase epsilon

Gene

Ptpre

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function By similarity. Acts as a negative regulator of insulin receptor (IR) signaling and is involved in insulin-induced glucose metabolism mainly through direct dephosphorylation and inactivation of IR in hepatocytes and liver.By similarity
    Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake By similarity.By similarity
    Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca2+.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei302 – 3021SubstrateBy similarity
    Active sitei334 – 3341Phosphocysteine intermediateBy similarity
    Binding sitei378 – 3781SubstrateBy similarity
    Active sitei629 – 6291Phosphocysteine intermediateBy similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: RGD

    GO - Biological processi

    1. negative regulation of insulin receptor signaling pathway Source: UniProtKB
    2. regulation of mast cell activation Source: Ensembl
    3. transmembrane receptor protein tyrosine phosphatase signaling pathway Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Receptor-type tyrosine-protein phosphatase epsilon (EC:3.1.3.48)
    Short name:
    Protein-tyrosine phosphatase epsilon
    Short name:
    R-PTP-epsilon
    Gene namesi
    Name:Ptpre
    Synonyms:Ptpe
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi620771. Ptpre.

    Subcellular locationi

    Isoform 2 : Cytoplasm By similarity
    Note: Predominantly cytoplasmic. A small fraction is also associated with nucleus and membrane. Insulin can induce translocation to the membrane By similarity.By similarity
    Isoform 3 : Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. intermediate filament cytoskeleton Source: Ensembl
    4. nucleus Source: Ensembl
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 699677Receptor-type tyrosine-protein phosphatase epsilonPRO_0000389638Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi31 – 311N-linked (GlcNAc...)Sequence Analysis
    Modified residuei695 – 6951PhosphotyrosineBy similarity

    Post-translational modificationi

    A catalytically active cytoplasmic form (p65) is produced by proteolytic cleavage of either isoform 1, isoform 2 or isoform 3.By similarity
    Isoform 1 and isoform 2 are phosphorylated on tyrosine residues by tyrosine kinase Neu.By similarity
    N-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiB2GV87.

    PTM databases

    PhosphoSiteiB2GV87.

    Expressioni

    Tissue specificityi

    Isoform 1 is highly expressed in the brain, lung, spleen and testis. Isoform 2 is highly expressed in thymus, spleen and lung. Isoform 1 and isoform 2 are expressed in primary hepatocytes.2 Publications

    Gene expression databases

    GenevestigatoriB2GV87.

    Interactioni

    Subunit structurei

    Monomer. Isoform 2: Homodimer. Can form oligomers. Dimerization is increased by oxidative stress and decreased by EGFR. Isoform 2 interacts with GRB2 By similarity.By similarity

    Protein-protein interaction databases

    IntActiB2GV87. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliB2GV87.
    SMRiB2GV87. Positions 110-690.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 4725ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini69 – 699631CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei48 – 6821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini134 – 393260Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini425 – 688264Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni334 – 3407Substrate bindingBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi52 – 6312Poly-LeuAdd
    BLAST

    Domaini

    The tyrosine-protein phosphatase 2 domain (D2) mediates dimerization. The extreme N- and C- termini of the D2 domain act to inhibit dimerization and removal of these sequences increases dimerization and inhibits enzyme activity By similarity.By similarity

    Sequence similaritiesi

    Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5599.
    GeneTreeiENSGT00590000082937.
    HOGENOMiHOG000231465.
    HOVERGENiHBG053758.
    InParanoidiQ63476.
    KOiK18033.
    OMAiIVIDAMI.
    OrthoDBiEOG7B31M8.
    PhylomeDBiB2GV87.

    Family and domain databases

    Gene3Di3.90.190.10. 2 hits.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    IPR016336. Tyr_Pase_rcpt_a/e-type.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 2 hits.
    [Graphical view]
    PIRSFiPIRSF002006. PTPR_alpha_epsilon. 1 hit.
    PRINTSiPR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 2 hits.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 2 hits.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 2 hits.
    PS50056. TYR_PHOSPHATASE_2. 2 hits.
    PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative promoter usage and alternative initiation. Align

    Isoform 1 (identifier: B2GV87-1) [UniParc]FASTAAdd to Basket

    Also known as: PTPeM, RPTPe, tm-PTPe

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPFCPLLLA SFSLSLATAG QGNDTTPTES NWTSTTAGPP DPGTSQPLLT    50
    WLLLPLLLLL FLLAAYFFRF RKQRKAVVNS NDKKMPNGIL EEQEQQRVML 100
    LSRSPSGPKK YFPIPVEHLE EEIRVRSADD CKRFREEFNS LPSGHIQGTF 150
    ELANKEENRE KNRYPNILPN DHCRVILSQL DGIPCSDYIN ASYIDGYKEK 200
    NKFIAAQGPK QETVNDFWRM VWEQRSATIV MLTNLKERKE EKCYQYWPDQ 250
    GCWTYGNIRV CVEDCVVLVD YTIRKFCIHP QLPDSCKAPR LVSQLHFTSW 300
    PDFGVPFTPI GMLKFLKKVK TLNPSHAGPI VVHCSAGVGR TGTFIVIDAM 350
    MDMIHSEQKV DVFEFVSRIR NQRPQMVQTD VQYTFIYQAL LEYYLYGDTE 400
    LDVSSLERHL QTLHGTATHF DKIGLEEEFR KLTNVRIMKE NMRTGNLPAN 450
    MKKARVIQII PYDFNRVILS MKRGQEFTDY INASFIDGYR QKDYFMATQG 500
    PLAHTVEDFW RMVWEWKSHT IVMLTEVQER EQDKCYQYWP TEGSVTHGDI 550
    TIEIKSDTLS EAISIRDFLV TFKQPLARQE EQVRMVRQFH FHGWPEVGIP 600
    TEGKGMIDLI AAVQKQQQQT GNHPITVHCS AGAGRTGTFI ALSNILERVK 650
    AEGLLDVFQA VKSLRLQRPH MVQTLEQYEF CYKVVQDFID IFSDYANFK 699

    Note: Produced by alternative promoter usage.

    Length:699
    Mass (Da):80,734
    Last modified:June 10, 2008 - v1
    Checksum:i799F63B840FF7C67
    GO
    Isoform 2 (identifier: B2GV87-2) [UniParc]FASTAAdd to Basket

    Also known as: PTPeC, cyt-PTPe

    The sequence of this isoform differs from the canonical sequence as follows:
         1-69: MEPFCPLLLA...LFLLAAYFFR → MSSRKNFSRLTW

    Note: Produced by alternative promoter usage.

    Show »
    Length:642
    Mass (Da):74,806
    Checksum:i8D2C47A09BAFE304
    GO
    Isoform 3 (identifier: B2GV87-3) [UniParc]FASTAAdd to Basket

    Also known as: p67

    The sequence of this isoform differs from the canonical sequence as follows:
         1-84: Missing.

    Note: Produced by alternative initiation at Met-85 of isoform 1.

    Show »
    Length:615
    Mass (Da):71,511
    Checksum:iD258D7FD1F75638E
    GO

    Sequence cautioni

    The sequence AAI66573.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41F → L in BAA11433. (PubMed:8579581)Curated
    Sequence conflicti573 – 5731K → T in BAA20333. (PubMed:8579581)Curated
    Sequence conflicti593 – 5931G → A in BAA20333. (PubMed:8579581)Curated
    Sequence conflicti610 – 6112IA → LS in BAA20333. (PubMed:8579581)Curated
    Sequence conflicti621 – 6211G → D in BAA20333. (PubMed:8579581)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8484Missing in isoform 3. 1 PublicationVSP_038488Add
    BLAST
    Alternative sequencei1 – 6969MEPFC…AYFFR → MSSRKNFSRLTW in isoform 2. 1 PublicationVSP_038489Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78610 mRNA. Translation: BAA20333.1.
    D78613 mRNA. Translation: BAA11433.1.
    CH473953 Genomic DNA. Translation: EDM11793.1.
    CH473953 Genomic DNA. Translation: EDM11794.1.
    BC166573 mRNA. Translation: AAI66573.1. Different initiation.
    CV110063 mRNA. No translation available.
    RefSeqiNP_446219.1. NM_053767.1. [B2GV87-1]
    XP_006230511.1. XM_006230449.1. [B2GV87-1]
    UniGeneiRn.107819.

    Genome annotation databases

    EnsembliENSRNOT00000021359; ENSRNOP00000021359; ENSRNOG00000015717. [B2GV87-1]
    ENSRNOT00000021379; ENSRNOP00000021379; ENSRNOG00000015717. [B2GV87-2]
    GeneIDi114767.
    KEGGirno:114767.
    UCSCiRGD:620771. rat. [B2GV87-1]

    Keywords - Coding sequence diversityi

    Alternative initiation, Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D78610 mRNA. Translation: BAA20333.1 .
    D78613 mRNA. Translation: BAA11433.1 .
    CH473953 Genomic DNA. Translation: EDM11793.1 .
    CH473953 Genomic DNA. Translation: EDM11794.1 .
    BC166573 mRNA. Translation: AAI66573.1 . Different initiation.
    CV110063 mRNA. No translation available.
    RefSeqi NP_446219.1. NM_053767.1. [B2GV87-1 ]
    XP_006230511.1. XM_006230449.1. [B2GV87-1 ]
    UniGenei Rn.107819.

    3D structure databases

    ProteinModelPortali B2GV87.
    SMRi B2GV87. Positions 110-690.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi B2GV87. 1 interaction.

    PTM databases

    PhosphoSitei B2GV87.

    Proteomic databases

    PaxDbi B2GV87.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000021359 ; ENSRNOP00000021359 ; ENSRNOG00000015717 . [B2GV87-1 ]
    ENSRNOT00000021379 ; ENSRNOP00000021379 ; ENSRNOG00000015717 . [B2GV87-2 ]
    GeneIDi 114767.
    KEGGi rno:114767.
    UCSCi RGD:620771. rat. [B2GV87-1 ]

    Organism-specific databases

    CTDi 5791.
    RGDi 620771. Ptpre.

    Phylogenomic databases

    eggNOGi COG5599.
    GeneTreei ENSGT00590000082937.
    HOGENOMi HOG000231465.
    HOVERGENi HBG053758.
    InParanoidi Q63476.
    KOi K18033.
    OMAi IVIDAMI.
    OrthoDBi EOG7B31M8.
    PhylomeDBi B2GV87.

    Miscellaneous databases

    NextBioi 618861.
    PROi B2GV87.

    Gene expression databases

    Genevestigatori B2GV87.

    Family and domain databases

    Gene3Di 3.90.190.10. 2 hits.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    IPR016336. Tyr_Pase_rcpt_a/e-type.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF002006. PTPR_alpha_epsilon. 1 hit.
    PRINTSi PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 2 hits.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 2 hits.
    PS50056. TYR_PHOSPHATASE_2. 2 hits.
    PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a novel cytoplasmic protein tyrosine phosphatase PTP epsilon."
      Nakamura K., Mizuno Y., Kikuchi K.
      Biochem. Biophys. Res. Commun. 218:726-732(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-297 (ISOFORM 1).
      Tissue: Spleen.
    2. "Distinct promoters control transmembrane and cytosolic protein tyrosine phosphatase epsilon expression during macrophage differentiation."
      Tanuma N., Nakamura K., Kikuchi K.
      Eur. J. Biochem. 259:46-54(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE PROMOTER USAGE, TISSUE SPECIFICITY.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.
    5. Strausberg R.L.
      Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-131 (ISOFORM 3).
      Tissue: Placenta.
    6. "Receptor-type protein tyrosine phosphatase epsilon (PTPepsilonM) is a negative regulator of insulin signaling in primary hepatocytes and liver."
      Nakagawa Y., Aoki N., Aoyama K., Shimizu H., Shimano H., Yamada N., Miyazaki H.
      Zool. Sci. 22:169-175(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION (ISOFORM 1), TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPTPRE_RAT
    AccessioniPrimary (citable) accession number: B2GV87
    Secondary accession number(s): Q63476, Q63477
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3