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Protein

DNA-binding protein RFX2

Gene

Rfx2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that acts as a key regulator of spermatogenesis (By similarity). Acts by regulating expression of genes required for the haploid phase during spermiogenesis, such as genes required for cilium assembly and function (By similarity). Recognizes and binds the X-box, a regulatory motif with DNA sequence 5'-GTNRCC(0-3N)RGYAAC-3' present on promoters (PubMed:14743396, PubMed:15526285, PubMed:16676351, PubMed:18247329). Probably activates transcription of the testis-specific histone gene HIST1H1T (PubMed:14743396, PubMed:15526285, PubMed:16676351, PubMed:18247329).By similarity4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi169 – 24476RFX-type winged-helixPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation, Differentiation, Spermatogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein RFX2
Alternative name(s):
Regulatory factor X 2
Gene namesi
Name:Rfx2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1588579. Rfx2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication
  • Cytoplasm 1 Publication

  • Note: Mainly expressed in the nucleus and at lower level in cytoplasm.1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692DNA-binding protein RFX2PRO_0000380696Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei386 – 3861PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiB2GV50.
PRIDEiB2GV50.

Expressioni

Tissue specificityi

Expressed at highest level in testis. Expressed at lower level in thymus. Also expressed in stomach, kidney, liver, brain and heart. Weakly expressed in spleen and lung (PubMed:16676351). Within testis, most abundantly present in spermatocytes: present from pachytene spermatocytes to early spermatids (at protein level) (PubMed:15229132, PubMed:16676351). Also present in non-germinal tissues (PubMed:16676351).2 Publications

Gene expression databases

ExpressionAtlasiB2GV50. baseline and differential.
GenevisibleiB2GV50. RN.

Interactioni

Subunit structurei

Homodimer; probably only forms homodimers in testis. Heterodimer; heterodimerizes with RFX1 and RFX3.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064610.

Structurei

3D structure databases

ProteinModelPortaliB2GV50.
SMRiB2GV50. Positions 169-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RFX family.PROSITE-ProRule annotation
Contains 1 RFX-type winged-helix DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3712. Eukaryota.
ENOG410XSHE. LUCA.
GeneTreeiENSGT00550000074532.
InParanoidiB2GV50.
KOiK09173.
OMAiGITSHKS.
OrthoDBiEOG7TF793.
PhylomeDBiB2GV50.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003150. DNA-bd_RFX.
IPR007668. RFX1_trans_act.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04589. RFX1_trans_act. 1 hit.
PF02257. RFX_DNA_binding. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS51526. RFX_DBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2GV50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNSEGGADS PATVALRPAA QPVPASPQRV LVQAAGSTPK GTPMQTLTLP
60 70 80 90 100
RVQPVPPQVQ HVYPAQVQYV EGGDAVYANG AIRAAYTYNP DPQLYAPSSA
110 120 130 140 150
ASYFETPGGA QVTVAASSPP AVPSHGMVGI TMDVSGTPIV SGAGTYLIHG
160 170 180 190 200
GMDSTRHSLA HTARSSPATL QWLLDNYETA EGVSLPRSSL YNHYLRHCQE
210 220 230 240 250
HKLEPVNAAS FGKLIRSVFM GLRTRRLGTR GNSKYHYYGI RLKPDSPLNR
260 270 280 290 300
LQEDTQYMAM RQQPTHQKPR YRPAQKSDSL GDGSAHSNMH STPEQAMAAQ
310 320 330 340 350
GQHHQQYIDV SHVFPEFPAP DLGSTLLQES VTLHDVKALQ LVYRRHCEAT
360 370 380 390 400
LDVVMNLQFQ YIEKLWLSFW NCKATSSDGR ASLPASDEEP EVTLLPKDKL
410 420 430 440 450
ISLCKCEPIL QWMRSCDHIL YQALVETLIP DVLRPVPSSL TQAIRNFAKS
460 470 480 490 500
LEGWLINAMS GFPQQVIQTK VGVVSAFAQT LRRYTSLNHL AQAARAVLQN
510 520 530 540 550
TSQINQMLSD LNRVDFANVQ EQASWVCQCE ESLVQRLEHD FKVTLQQQSS
560 570 580 590 600
LDQWASWLDN VVTQVLKQHA GSPSFPKAAR QFLLKWSFYS SMVIRDLTLR
610 620 630 640 650
SAASFGSFHL IRLLYDEYMF YLVEHRVAQA TGETPIAVMG EFNDLASLSL
660 670 680 690
TLLDKEDIGD GHSSEADVDG RSLGEPLVKR ERSDPSHPLQ GI
Length:692
Mass (Da):76,537
Last modified:June 10, 2008 - v1
Checksum:iAB5EA2C1105A8017
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474092 Genomic DNA. Translation: EDL83603.1.
BC166527 mRNA. Translation: AAI66527.1.
RefSeqiNP_001100347.1. NM_001106877.1.
UniGeneiRn.35756.

Genome annotation databases

EnsembliENSRNOT00000073025; ENSRNOP00000064610; ENSRNOG00000045846.
GeneIDi301121.
KEGGirno:301121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474092 Genomic DNA. Translation: EDL83603.1.
BC166527 mRNA. Translation: AAI66527.1.
RefSeqiNP_001100347.1. NM_001106877.1.
UniGeneiRn.35756.

3D structure databases

ProteinModelPortaliB2GV50.
SMRiB2GV50. Positions 169-243.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064610.

Proteomic databases

PaxDbiB2GV50.
PRIDEiB2GV50.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000073025; ENSRNOP00000064610; ENSRNOG00000045846.
GeneIDi301121.
KEGGirno:301121.

Organism-specific databases

CTDi5990.
RGDi1588579. Rfx2.

Phylogenomic databases

eggNOGiKOG3712. Eukaryota.
ENOG410XSHE. LUCA.
GeneTreeiENSGT00550000074532.
InParanoidiB2GV50.
KOiK09173.
OMAiGITSHKS.
OrthoDBiEOG7TF793.
PhylomeDBiB2GV50.

Miscellaneous databases

NextBioi648184.
PROiB2GV50.

Gene expression databases

ExpressionAtlasiB2GV50. baseline and differential.
GenevisibleiB2GV50. RN.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR003150. DNA-bd_RFX.
IPR007668. RFX1_trans_act.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04589. RFX1_trans_act. 1 hit.
PF02257. RFX_DNA_binding. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS51526. RFX_DBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. "RFX2 is a potential transcriptional regulatory factor for histone H1t and other genes expressed during the meiotic phase of spermatogenesis."
    Horvath G.C., Kistler W.S., Kistler M.K.
    Biol. Reprod. 71:1551-1559(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Regulatory factor X2 (RFX2) binds to the H1t/TE1 promoter element and activates transcription of the testis-specific histone H1t gene."
    Wolfe S.A., Wilkerson D.C., Prado S., Grimes S.R.
    J. Cell. Biochem. 91:375-383(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  5. "Transcriptional activation of the testis-specific histone H1t gene by RFX2 may require both proximal promoter X-box elements."
    Grimes S.R., Prado S., Wolfe S.A.
    J. Cell. Biochem. 94:317-326(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  6. "Transcription factor RFX2 is abundant in rat testis and enriched in nuclei of primary spermatocytes where it appears to be required for transcription of the testis-specific histone H1t gene."
    Wolfe S.A., van Wert J., Grimes S.R.
    J. Cell. Biochem. 99:735-746(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "Binding of RFX2 and NF-Y to the testis-specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes."
    VanWert J.M., Wolfe S.A., Grimes S.R.
    J. Cell. Biochem. 104:1087-1101(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  8. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-386, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRFX2_RAT
AccessioniPrimary (citable) accession number: B2GV50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: May 11, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.