Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 UFM1-protein ligase 1

Gene

Ufl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, a post-translational modification on lysine residues of proteins that may play a crucial role in a number of cellular processes. Mediates DDRGK1 ufmylation and may regulate the proteasomal degradation of DDRGK1 and CDK5RAP3 thereby modulating NF-kappa-B signaling. May also through TRIP4 ufmylation play a role in nuclear receptors-mediated transcription. May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
E3 UFM1-protein ligase 1Curated (EC:2.3.2.-By similarity)
Alternative name(s):
E3 UFM1-protein transferase 1Curated
Gene namesi
Name:Ufl1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1309308 Ufl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003918752 – 793E3 UFM1-protein ligase 1Add BLAST792

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei433Omega-N-methylarginineBy similarity1
Modified residuei458PhosphoserineCombined sources1
Modified residuei752PhosphoserineBy similarity1
Modified residuei753PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated, leading to its degradation by the proteasome. Interaction with CDK5RAP3 protects each other against ubiquitination and degradation via the proteasome.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiB2GV24
PeptideAtlasiB2GV24
PRIDEiB2GV24

PTM databases

iPTMnetiB2GV24
PhosphoSitePlusiB2GV24

Expressioni

Tissue specificityi

Ubiquitously expressed (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000007831
ExpressionAtlasiB2GV24 baseline and differential
GenevisibleiB2GV24 RN

Interactioni

Subunit structurei

Interacts with DDRGK1. Interacts with UFC1. Interacts with RELA. Interacts with TRIP4. Interacts with CDK5RAP3; the interaction is direct.By similarity

Protein-protein interaction databases

IntActiB2GV24, 2 interactors
MINTiB2GV24
STRINGi10116.ENSRNOP00000010421

Structurei

3D structure databases

SMRiB2GV24
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 212Required for E3 UFM1-protein ligase activityBy similarityAdd BLAST211
Regioni2 – 200Mediates interaction with DDRGK1By similarityAdd BLAST199
Regioni121 – 250Involved in CDK5RAP3-bindingBy similarityAdd BLAST130
Regioni200 – 400Mediates interaction with TRIP4By similarityAdd BLAST201

Sequence similaritiesi

Belongs to the UFL1 family.Curated

Phylogenomic databases

eggNOGiKOG2235 Eukaryota
ENOG410XSRX LUCA
GeneTreeiENSGT00390000002112
HOGENOMiHOG000007320
HOVERGENiHBG060009
InParanoidiB2GV24
OMAiQILFQHR
OrthoDBiEOG091G03AN
PhylomeDBiB2GV24
TreeFamiTF319116

Family and domain databases

InterProiView protein in InterPro
IPR018611 E3_UFM1_ligase_1
PfamiView protein in Pfam
PF09743 E3_UFM1_ligase, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2GV24-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADAWEEIRR LAADFQRAQF AESTQRLSER NCIEIVNKLI SQKQLEVVHT
60 70 80 90 100
LDGKEYITPA QISKEMRDEL HVRGGRVNIV DLQQVINVDL THIENRVGDI
110 120 130 140 150
IKSEKHVQMV LGQLVDENYL DRLSEEVNDK LQESGQVTVS ELCKTYDLPG
160 170 180 190 200
DFLTQALTQR LGRIINGHLD LDNRGVIFTE AFVARHKARI RGLFSAITRP
210 220 230 240 250
TAVNSLVSKY GFQEQLLYSV LEELVSTGRL RGTVVGGRQD KAVFVPDIYS
260 270 280 290 300
RTQSTWVDSF FRQNGYLEFD ALSRLGIPDA VNYIKKRYKN TPLLFLKATC
310 320 330 340 350
VGQGLVDQVE ASVEEAISSG TWVDVSPLLP SSLSVEDAAM LLQQVMRPFG
360 370 380 390 400
KHASATVFSD TVVVSEKFIN DCTKLFSERM HQKAEKEMKN NPVHLITEED
410 420 430 440 450
LKQISILESV NTNKKDKKDE RRKKATEGSG SVRGGGGGNA REYKIKKVKK
460 470 480 490 500
KGRKDEDSDD ESQSSHAGKK KPDITFMFQD EIEGCLRKHI PDAPEEFISE
510 520 530 540 550
LAEHLIKPLN KMYLEVVRSV FMSSTSASGT GRKRTIKDLQ EEVSNLYNNI
560 570 580 590 600
RLFEKGMKYF ADDTQTALTK HLLKTVCTDI TNLMFNFLAS DLMMAVEDPA
610 620 630 640 650
TITSDMRKKI LSKLTEETKV ALTKLHNSLN EKSIEDFLSC LDSATEACDI
660 670 680 690 700
MVKKGDKKRE RQILFQHRQA LADQLKVTED PALILHLTSV LLFQFSTHSM
710 720 730 740 750
LHAPGRCVPQ IIAFLHNKIP EDQHTLLVKY QGLVVKQLVS QNKKSGQGED
760 770 780 790
PSSDDLDKEQ HDVTNTTRKE LQELSLSIKD LVLKPRKSSV TEE
Length:793
Mass (Da):89,585
Last modified:June 10, 2008 - v1
Checksum:iF99BB2764FD8F7DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166498 mRNA Translation: AAI66498.1
RefSeqiNP_001119751.1, NM_001126279.1
UniGeneiRn.12784

Genome annotation databases

EnsembliENSRNOT00000010421; ENSRNOP00000010421; ENSRNOG00000007831
GeneIDi313115
KEGGirno:313115
UCSCiRGD:1309308 rat

Similar proteinsi

Entry informationi

Entry nameiUFL1_RAT
AccessioniPrimary (citable) accession number: B2GV24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: June 10, 2008
Last modified: April 25, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health