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Protein

Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial

Gene

Oxct1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity).By similarity

Catalytic activityi

Succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA.PROSITE-ProRule annotationBy similarity

Pathwayi: succinyl-CoA degradation

This protein is involved in step 1 of the subpathway that synthesizes acetoacetyl-CoA from succinyl-CoA.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (Oxct1), Succinyl-CoA:3-ketoacid coenzyme A transferase 2A, mitochondrial (Oxct2a)
This subpathway is part of the pathway succinyl-CoA degradation, which is itself part of Ketone metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetyl-CoA from succinyl-CoA, the pathway succinyl-CoA degradation and in Ketone metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3445-glutamyl coenzyme A thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • 3-oxoacid CoA-transferase activity Source: RGD
  • protein homodimerization activity Source: RGD

GO - Biological processi

  • adipose tissue development Source: RGD
  • brain development Source: RGD
  • cellular ketone body metabolic process Source: RGD
  • heart development Source: RGD
  • ketone body catabolic process Source: Ensembl
  • ketone catabolic process Source: Ensembl
  • positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: RGD
  • response to activity Source: RGD
  • response to drug Source: RGD
  • response to ethanol Source: RGD
  • response to hormone Source: RGD
  • response to nutrient Source: RGD
  • response to starvation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BRENDAi2.8.3.5. 5301.
ReactomeiR-RNO-77108. Utilization of Ketone Bodies.
UniPathwayiUPA00929; UER00894.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial (EC:2.8.3.5)
Alternative name(s):
3-oxoacid CoA-transferase 1
Somatic-type succinyl-CoA:3-oxoacid CoA-transferase
Short name:
SCOT-s
Gene namesi
Name:Oxct1Imported
Synonyms:OxctBy similarity, ScotBy similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1584008. Oxct1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39MitochondrionBy similarityAdd BLAST39
ChainiPRO_000034912740 – 520Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrialAdd BLAST481

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei170PhosphoserineBy similarity1
Modified residuei185N6-succinyllysineBy similarity1
Modified residuei418N6-succinyllysineBy similarity1
Modified residuei421N6-succinyllysineBy similarity1
Modified residuei455N6-succinyllysineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiB2GV06.
PeptideAtlasiB2GV06.
PRIDEiB2GV06.

PTM databases

iPTMnetiB2GV06.
PhosphoSitePlusiB2GV06.

Expressioni

Gene expression databases

BgeeiENSRNOG00000043094.
GenevisibleiB2GV06. RN.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063646.

Structurei

3D structure databases

ProteinModelPortaliB2GV06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-oxoacid CoA-transferase family.Sequence analysis

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3822. Eukaryota.
COG1788. LUCA.
COG2057. LUCA.
GeneTreeiENSGT00390000009130.
HOGENOMiHOG000221244.
HOVERGENiHBG002310.
InParanoidiB2GV06.
KOiK01027.
OMAiASDLKVM.
OrthoDBiEOG091G0D3X.
PhylomeDBiB2GV06.

Family and domain databases

InterProiIPR012792. 3-oxoacid_CoA-transf_A.
IPR012791. 3-oxoacid_CoA-transf_B.
IPR014388. 3-oxoacid_CoA-transferase.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
IPR004163. CoA_transf_BS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 2 hits.
[Graphical view]
PIRSFiPIRSF000858. SCOT-t. 1 hit.
SMARTiSM00882. CoA_trans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR02429. pcaI_scoA_fam. 1 hit.
TIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEiPS01273. COA_TRANSF_1. 1 hit.
PS01274. COA_TRANSF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B2GV06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALKLLSSG LRLCASARNS RGALHKGCAC YFSVSTRHHT KFYTDPVEAV
60 70 80 90 100
KDIPNGATLL VGGFGLCGIP ENLIGALLKT GVKDLTAVSN NAGVDNFGLG
110 120 130 140 150
LLLRSKQIKR MISSYVGENA EFERQFLSGE LEVELTPQGT LAERIRAGGA
160 170 180 190 200
GVPAFYTSTG YGTLVQEGGS PIKYNKDGSV AIASKPREVR EFRGQHFILE
210 220 230 240 250
EAITGDFALV KAWKADRAGN VIFRKSARNF NLPMCKAAGT TVVEVEEIVD
260 270 280 290 300
IGSFAPEDIH IPKIYVHRLI KGEKYEKRIE RLSLRKEGEG KAKSGKPGED
310 320 330 340 350
VRERIIKRAA LEFEDGMYAN LGIGIPLLAS NFISPNMTVH LQSENGVLGL
360 370 380 390 400
GPYPLKDEAD ADLINAGKET VTVLPGASFF SSDESFAMIR GGHVNLTMLG
410 420 430 440 450
AMQVSKYGDL ANWMIPGKMV KGMGGAMDLV SSSKTKVVVT MEHSAKGNAH
460 470 480 490 500
KIMEKCTLPL TGKQCVNRII TEKGVFDVDK KNGLTLIELW EGLTVDDIRK
510 520
STGCDFAVSP NLMPMQQIST
Length:520
Mass (Da):56,204
Last modified:June 10, 2008 - v1
Checksum:iD34D4F805665BC63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474048 Genomic DNA. Translation: EDL75738.1.
BC166478 mRNA. Translation: AAI66478.1.
RefSeqiNP_001121052.1. NM_001127580.1.
UniGeneiRn.98472.

Genome annotation databases

EnsembliENSRNOT00000065313; ENSRNOP00000063646; ENSRNOG00000043094.
GeneIDi690163.
KEGGirno:690163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474048 Genomic DNA. Translation: EDL75738.1.
BC166478 mRNA. Translation: AAI66478.1.
RefSeqiNP_001121052.1. NM_001127580.1.
UniGeneiRn.98472.

3D structure databases

ProteinModelPortaliB2GV06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000063646.

PTM databases

iPTMnetiB2GV06.
PhosphoSitePlusiB2GV06.

Proteomic databases

PaxDbiB2GV06.
PeptideAtlasiB2GV06.
PRIDEiB2GV06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000065313; ENSRNOP00000063646; ENSRNOG00000043094.
GeneIDi690163.
KEGGirno:690163.

Organism-specific databases

CTDi5019.
RGDi1584008. Oxct1.

Phylogenomic databases

eggNOGiKOG3822. Eukaryota.
COG1788. LUCA.
COG2057. LUCA.
GeneTreeiENSGT00390000009130.
HOGENOMiHOG000221244.
HOVERGENiHBG002310.
InParanoidiB2GV06.
KOiK01027.
OMAiASDLKVM.
OrthoDBiEOG091G0D3X.
PhylomeDBiB2GV06.

Enzyme and pathway databases

UniPathwayiUPA00929; UER00894.
BRENDAi2.8.3.5. 5301.
ReactomeiR-RNO-77108. Utilization of Ketone Bodies.

Miscellaneous databases

PROiB2GV06.

Gene expression databases

BgeeiENSRNOG00000043094.
GenevisibleiB2GV06. RN.

Family and domain databases

InterProiIPR012792. 3-oxoacid_CoA-transf_A.
IPR012791. 3-oxoacid_CoA-transf_B.
IPR014388. 3-oxoacid_CoA-transferase.
IPR004165. CoA_trans_fam_I.
IPR004164. CoA_transf_AS.
IPR004163. CoA_transf_BS.
[Graphical view]
PANTHERiPTHR13707. PTHR13707. 1 hit.
PfamiPF01144. CoA_trans. 2 hits.
[Graphical view]
PIRSFiPIRSF000858. SCOT-t. 1 hit.
SMARTiSM00882. CoA_trans. 2 hits.
[Graphical view]
TIGRFAMsiTIGR02429. pcaI_scoA_fam. 1 hit.
TIGR02428. pcaJ_scoB_fam. 1 hit.
PROSITEiPS01273. COA_TRANSF_1. 1 hit.
PS01274. COA_TRANSF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSCOT1_RAT
AccessioniPrimary (citable) accession number: B2GV06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: June 10, 2008
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.