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B2GUZ1

- UBP4_RAT

UniProt

B2GUZ1 - UBP4_RAT

Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

Usp4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER.1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei311 – 3111By similarity
    Metal bindingi461 – 4611ZincBy similarity
    Metal bindingi464 – 4641ZincBy similarity
    Metal bindingi797 – 7971ZincBy similarity
    Metal bindingi800 – 8001ZincBy similarity
    Active sitei879 – 8791By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ubiquitin-specific protease activity Source: UniProtKB
    3. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. negative regulation of protein ubiquitination Source: UniProtKB
    2. protein deubiquitination Source: UniProtKB
    3. protein localization to cell surface Source: UniProtKB
    4. regulation of protein stability Source: UniProtKB
    5. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 4
    Ubiquitin thioesterase 4
    Ubiquitin-specific-processing protease 4
    Gene namesi
    Name:Usp4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi1587387. Usp4.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 961961Ubiquitin carboxyl-terminal hydrolase 4PRO_0000396806Add
    BLAST

    Post-translational modificationi

    Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated By similarity.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PRIDEiB2GUZ1.

    PTM databases

    PhosphoSiteiB2GUZ1.

    Expressioni

    Tissue specificityi

    Expressed in hippocampus and striatum (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriB2GUZ1.

    Interactioni

    Subunit structurei

    Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi253348. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliB2GUZ1.
    SMRiB2GUZ1. Positions 1-124.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 122112DUSPPROSITE-ProRule annotationAdd
    BLAST
    Domaini142 – 22685Ubiquitin-like 1Add
    BLAST
    Domaini302 – 921620USPAdd
    BLAST
    Domaini483 – 57189Ubiquitin-like 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni405 – 4073Necessary for interaction with RBL2By similarity
    Regioni459 – 4635Necessary for interaction with RB1 and RBL2By similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi133 – 1419Nuclear export signalBy similarity
    Motifi765 – 7706Nuclear localization signalBy similarity

    Domaini

    The Ubiquitin-like domain 2 inserts into the catalytic domain and competes with the ubiquitin substrate, partially inhibiting DUB activity.By similarity

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP4 subfamily.Curated
    Contains 1 DUSP domain.PROSITE-ProRule annotation
    Contains 2 ubiquitin-like domains.Curated
    Contains 1 USP domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    GeneTreeiENSGT00670000097750.
    HOVERGENiHBG000864.
    KOiK11835.
    OMAiVEFPTRG.
    OrthoDBiEOG77Q4VW.
    PhylomeDBiB2GUZ1.

    Family and domain databases

    Gene3Di3.30.2230.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028135. Ub_USP-typ.
    IPR028889. UCH/PAN2.
    IPR028134. USP.
    [Graphical view]
    PANTHERiPTHR24006:SF360. PTHR24006:SF360. 1 hit.
    PfamiPF06337. DUSP. 1 hit.
    PF14836. Ubiquitin_3. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 1 hit.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 1 hit.
    PROSITEiPS51283. DUSP. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2GUZ1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEGRGTHER PDVETQKTEL GALMGTTLQR GAQWYLIDSR WFKQWKKYVG    50
    FDSWDMYNVG EHNLFPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPTEA 100
    WNKLLNWYGC VEGQQPIVRK VVEHGLFVKH CKVEVYLLEL KLCENSDPTN 150
    VLSCHFSKAD TIATIEKEMR KLFNIPAERE TRLWNKYMSN TYEQLSKLDN 200
    TIQDAGLYQG QVLVIEPQNE DGTWPRQTLQ SKSSTAPSRN FTTSSKPSAS 250
    PYSSMSASLI ANGDSTNSSG MHNSGVSRGG AGFSASYNCQ EPPSPHIQPG 300
    LCGLGNLGNT CFMNSALQCL SNTGPLTEYF LKDEYEAEIN RDNPLGMKGE 350
    IAEAYAELIK QMWSGRDTHV APRMFKTQVG RFAPQFSGYQ QQDSQELLAF 400
    ILDGLHEDLN RVKKKPYLEP KDANGRPDAV VAKEAWENHR LRNDSVIVDT 450
    FHGLFKSTLV CPECAKVSVT FDPFCYLTLP LPLKKDRIME VFLVPADPHC 500
    RPIQYRVTVP LMGAISDLCE ALSKLSGIAA ENMVVTDVYN HRFHKIFQMD 550
    EGLSHITPRD DIFVYEICTT PMDGSEYITL PVYFREKKSR PSSTSSGAVL 600
    YGQPLLVSVP KHRLTLESLY QAVCERISRY IKQPLPEEFL SSPLEPGACN 650
    GSRGSYEGDE EEMDHQEEGK EQLSEVEESG EDSQGGDPTE TTQKAKGPPR 700
    HKRLFTFSLV NSCGTADINS LATDGKLLKL NSRSTLAIDW DSETRSLYFD 750
    EQESEACEKH TSMSQPQKKK KAAIALRECI ELFTTMETLG EHDPWYCPTC 800
    KKHQQATKKF DLWSLPKILV VHLKRFSYNR YWRDKLDTVV EFPVRALNMS 850
    EFVCDRAARP YVYDLIAVSN HYGAMGVGHY TAYAKNRLNG KWYYFDDSSV 900
    SLASEDQIVT KAAYVLFYQR RDDECPSTSS PVSFPGSDGG AKLSSSQQDL 950
    GEEEAYTMDT N 961
    Length:961
    Mass (Da):108,373
    Last modified:June 10, 2008 - v1
    Checksum:iD6DD975327451B9F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC166460 mRNA. Translation: AAI66460.1.
    CH473954 Genomic DNA. Translation: EDL77177.1.
    RefSeqiNP_001128484.1. NM_001135012.1.
    XP_006243773.1. XM_006243711.1.
    UniGeneiRn.92126.

    Genome annotation databases

    EnsembliENSRNOT00000073725; ENSRNOP00000066449; ENSRNOG00000046486.
    GeneIDi290864.
    KEGGirno:290864.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC166460 mRNA. Translation: AAI66460.1 .
    CH473954 Genomic DNA. Translation: EDL77177.1 .
    RefSeqi NP_001128484.1. NM_001135012.1.
    XP_006243773.1. XM_006243711.1.
    UniGenei Rn.92126.

    3D structure databases

    ProteinModelPortali B2GUZ1.
    SMRi B2GUZ1. Positions 1-124.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 253348. 1 interaction.

    PTM databases

    PhosphoSitei B2GUZ1.

    Proteomic databases

    PRIDEi B2GUZ1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000073725 ; ENSRNOP00000066449 ; ENSRNOG00000046486 .
    GeneIDi 290864.
    KEGGi rno:290864.

    Organism-specific databases

    CTDi 7375.
    RGDi 1587387. Usp4.

    Phylogenomic databases

    GeneTreei ENSGT00670000097750.
    HOVERGENi HBG000864.
    KOi K11835.
    OMAi VEFPTRG.
    OrthoDBi EOG77Q4VW.
    PhylomeDBi B2GUZ1.

    Miscellaneous databases

    NextBioi 631779.

    Gene expression databases

    Genevestigatori B2GUZ1.

    Family and domain databases

    Gene3Di 3.30.2230.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028135. Ub_USP-typ.
    IPR028889. UCH/PAN2.
    IPR028134. USP.
    [Graphical view ]
    PANTHERi PTHR24006:SF360. PTHR24006:SF360. 1 hit.
    Pfami PF06337. DUSP. 1 hit.
    PF14836. Ubiquitin_3. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 1 hit.
    PROSITEi PS51283. DUSP. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Brown Norway.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    3. "The ubiquitin-specific protease Usp4 regulates the cell surface level of the A2A receptor."
      Milojevic T., Reiterer V., Stefan E., Korkhov V.M., Dorostkar M.M., Ducza E., Ogris E., Boehm S., Freissmuth M., Nanoff C.
      Mol. Pharmacol. 69:1083-1094(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiUBP4_RAT
    AccessioniPrimary (citable) accession number: B2GUZ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3