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B2GUZ1

- UBP4_RAT

UniProt

B2GUZ1 - UBP4_RAT

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Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene

Usp4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.By similarity1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei311 – 3111By similarity
Metal bindingi461 – 4611ZincBy similarity
Metal bindingi464 – 4641ZincBy similarity
Metal bindingi797 – 7971ZincBy similarity
Metal bindingi800 – 8001ZincBy similarity
Active sitei879 – 8791By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: UniProtKB
  3. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. negative regulation of protein ubiquitination Source: UniProtKB
  2. protein deubiquitination Source: UniProtKB
  3. protein localization to cell surface Source: UniProtKB
  4. regulation of protein stability Source: UniProtKB
  5. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:Usp4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi1587387. Usp4.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
  3. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 961961Ubiquitin carboxyl-terminal hydrolase 4PRO_0000396806Add
BLAST

Post-translational modificationi

Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiB2GUZ1.

PTM databases

PhosphoSiteiB2GUZ1.

Expressioni

Tissue specificityi

Expressed in hippocampus and striatum (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiB2GUZ1. baseline.
GenevestigatoriB2GUZ1.

Interactioni

Subunit structurei

Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with RBL1 and RBL2. Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with SART3; recruits USP4 to its substrate PRPF3.By similarity

Protein-protein interaction databases

BioGridi253348. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliB2GUZ1.
SMRiB2GUZ1. Positions 1-124.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 122112DUSPPROSITE-ProRule annotationAdd
BLAST
Domaini142 – 22685Ubiquitin-like 1Add
BLAST
Domaini302 – 921620USPAdd
BLAST
Domaini483 – 57189Ubiquitin-like 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 216190Necessary for interaction with SART3By similarityAdd
BLAST
Regioni405 – 4073Necessary for interaction with RBL2By similarity
Regioni459 – 4635Necessary for interaction with RB1 and RBL2By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi133 – 1419Nuclear export signalBy similarity
Motifi765 – 7706Nuclear localization signalBy similarity

Domaini

The Ubiquitin-like domain 2 inserts into the catalytic domain and competes with the ubiquitin substrate, partially inhibiting DUB activity.By similarity

Sequence similaritiesi

Belongs to the peptidase C19 family. USP4 subfamily.Curated
Contains 1 DUSP domain.PROSITE-ProRule annotation
Contains 2 ubiquitin-like domains.Curated
Contains 1 USP domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00670000097750.
HOVERGENiHBG000864.
InParanoidiB2GUZ1.
KOiK11835.
OMAiVEFPTRG.
OrthoDBiEOG77Q4VW.
PhylomeDBiB2GUZ1.

Family and domain databases

Gene3Di3.30.2230.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
IPR028134. USP.
[Graphical view]
PANTHERiPTHR24006:SF360. PTHR24006:SF360. 1 hit.
PfamiPF06337. DUSP. 1 hit.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 1 hit.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 1 hit.
PROSITEiPS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2GUZ1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEGRGTHER PDVETQKTEL GALMGTTLQR GAQWYLIDSR WFKQWKKYVG
60 70 80 90 100
FDSWDMYNVG EHNLFPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPTEA
110 120 130 140 150
WNKLLNWYGC VEGQQPIVRK VVEHGLFVKH CKVEVYLLEL KLCENSDPTN
160 170 180 190 200
VLSCHFSKAD TIATIEKEMR KLFNIPAERE TRLWNKYMSN TYEQLSKLDN
210 220 230 240 250
TIQDAGLYQG QVLVIEPQNE DGTWPRQTLQ SKSSTAPSRN FTTSSKPSAS
260 270 280 290 300
PYSSMSASLI ANGDSTNSSG MHNSGVSRGG AGFSASYNCQ EPPSPHIQPG
310 320 330 340 350
LCGLGNLGNT CFMNSALQCL SNTGPLTEYF LKDEYEAEIN RDNPLGMKGE
360 370 380 390 400
IAEAYAELIK QMWSGRDTHV APRMFKTQVG RFAPQFSGYQ QQDSQELLAF
410 420 430 440 450
ILDGLHEDLN RVKKKPYLEP KDANGRPDAV VAKEAWENHR LRNDSVIVDT
460 470 480 490 500
FHGLFKSTLV CPECAKVSVT FDPFCYLTLP LPLKKDRIME VFLVPADPHC
510 520 530 540 550
RPIQYRVTVP LMGAISDLCE ALSKLSGIAA ENMVVTDVYN HRFHKIFQMD
560 570 580 590 600
EGLSHITPRD DIFVYEICTT PMDGSEYITL PVYFREKKSR PSSTSSGAVL
610 620 630 640 650
YGQPLLVSVP KHRLTLESLY QAVCERISRY IKQPLPEEFL SSPLEPGACN
660 670 680 690 700
GSRGSYEGDE EEMDHQEEGK EQLSEVEESG EDSQGGDPTE TTQKAKGPPR
710 720 730 740 750
HKRLFTFSLV NSCGTADINS LATDGKLLKL NSRSTLAIDW DSETRSLYFD
760 770 780 790 800
EQESEACEKH TSMSQPQKKK KAAIALRECI ELFTTMETLG EHDPWYCPTC
810 820 830 840 850
KKHQQATKKF DLWSLPKILV VHLKRFSYNR YWRDKLDTVV EFPVRALNMS
860 870 880 890 900
EFVCDRAARP YVYDLIAVSN HYGAMGVGHY TAYAKNRLNG KWYYFDDSSV
910 920 930 940 950
SLASEDQIVT KAAYVLFYQR RDDECPSTSS PVSFPGSDGG AKLSSSQQDL
960
GEEEAYTMDT N
Length:961
Mass (Da):108,373
Last modified:June 10, 2008 - v1
Checksum:iD6DD975327451B9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166460 mRNA. Translation: AAI66460.1.
CH473954 Genomic DNA. Translation: EDL77177.1.
RefSeqiNP_001128484.1. NM_001135012.1.
UniGeneiRn.92126.

Genome annotation databases

EnsembliENSRNOT00000073725; ENSRNOP00000066449; ENSRNOG00000046486.
GeneIDi290864.
KEGGirno:290864.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166460 mRNA. Translation: AAI66460.1 .
CH473954 Genomic DNA. Translation: EDL77177.1 .
RefSeqi NP_001128484.1. NM_001135012.1.
UniGenei Rn.92126.

3D structure databases

ProteinModelPortali B2GUZ1.
SMRi B2GUZ1. Positions 1-124.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 253348. 1 interaction.

PTM databases

PhosphoSitei B2GUZ1.

Proteomic databases

PRIDEi B2GUZ1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000073725 ; ENSRNOP00000066449 ; ENSRNOG00000046486 .
GeneIDi 290864.
KEGGi rno:290864.

Organism-specific databases

CTDi 7375.
RGDi 1587387. Usp4.

Phylogenomic databases

GeneTreei ENSGT00670000097750.
HOVERGENi HBG000864.
InParanoidi B2GUZ1.
KOi K11835.
OMAi VEFPTRG.
OrthoDBi EOG77Q4VW.
PhylomeDBi B2GUZ1.

Miscellaneous databases

NextBioi 631779.

Gene expression databases

ExpressionAtlasi B2GUZ1. baseline.
Genevestigatori B2GUZ1.

Family and domain databases

Gene3Di 3.30.2230.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
IPR028134. USP.
[Graphical view ]
PANTHERi PTHR24006:SF360. PTHR24006:SF360. 1 hit.
Pfami PF06337. DUSP. 1 hit.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 1 hit.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 1 hit.
PROSITEi PS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "The ubiquitin-specific protease Usp4 regulates the cell surface level of the A2A receptor."
    Milojevic T., Reiterer V., Stefan E., Korkhov V.M., Dorostkar M.M., Ducza E., Ogris E., Boehm S., Freissmuth M., Nanoff C.
    Mol. Pharmacol. 69:1083-1094(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiUBP4_RAT
AccessioniPrimary (citable) accession number: B2GUZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: June 10, 2008
Last modified: November 26, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3