SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B2GUZ1

- UBP4_RAT

UniProt

B2GUZ1 - UBP4_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ubiquitin carboxyl-terminal hydrolase 4

Gene
Usp4
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolase that deubiquitinates target proteins such as the receptor ADORA2A, PDPK1 and TRIM21. Deubiquitination of ADORA2A increases the amount of functional receptor at the cell surface. Plays a role in the regulation of quality control in the ER.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei311 – 3111 By similarity
Metal bindingi461 – 4611Zinc By similarity
Metal bindingi464 – 4641Zinc By similarity
Metal bindingi797 – 7971Zinc By similarity
Metal bindingi800 – 8001Zinc By similarity
Active sitei879 – 8791 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ubiquitin-specific protease activity Source: UniProtKB
  3. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. negative regulation of protein ubiquitination Source: UniProtKB
  2. protein deubiquitination Source: UniProtKB
  3. protein localization to cell surface Source: UniProtKB
  4. regulation of protein stability Source: UniProtKB
  5. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 4 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 4
Ubiquitin thioesterase 4
Ubiquitin-specific-processing protease 4
Gene namesi
Name:Usp4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi1587387. Usp4.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Shuttles between the nucleus and cytoplasm. Exported to the cytoplasm in a CRM1-dependent manner and recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The relative amounts found in the nucleus and cytoplasm vary according to the cell type By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 961961Ubiquitin carboxyl-terminal hydrolase 4PRO_0000396806Add
BLAST

Post-translational modificationi

Monoubiquitinated by TRIM21. Ubiquitination does not lead to its proteasomal degradation. Autodeubiquitinated By similarity.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiB2GUZ1.

PTM databases

PhosphoSiteiB2GUZ1.

Expressioni

Tissue specificityi

Expressed in hippocampus and striatum (at protein level).1 Publication

Gene expression databases

GenevestigatoriB2GUZ1.

Interactioni

Subunit structurei

Interacts with RB1 (both dephosphorylated and hypophosphorylated forms). Interacts with ADORA2A (via cytoplasmic C-terminus); the interaction is direct. Interacts with RB1, RBL1 and RBL2 By similarity.

Protein-protein interaction databases

BioGridi253348. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliB2GUZ1.
SMRiB2GUZ1. Positions 1-124.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 122112DUSPAdd
BLAST
Domaini142 – 22685Ubiquitin-like 1Add
BLAST
Domaini302 – 921620USPAdd
BLAST
Domaini483 – 57189Ubiquitin-like 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni405 – 4073Necessary for interaction with RBL2 By similarity
Regioni459 – 4635Necessary for interaction with RB1 and RBL2 By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi133 – 1419Nuclear export signal By similarity
Motifi765 – 7706Nuclear localization signal By similarity

Domaini

The Ubiquitin-like domain 2 inserts into the catalytic domain and competes with the ubiquitin substrate, partially inhibiting DUB activity By similarity.

Sequence similaritiesi

Contains 1 DUSP domain.
Contains 1 USP domain.

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00670000097750.
HOVERGENiHBG000864.
KOiK11835.
OMAiVEFPTRG.
OrthoDBiEOG77Q4VW.
PhylomeDBiB2GUZ1.

Family and domain databases

Gene3Di3.30.2230.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
IPR028134. USP.
[Graphical view]
PANTHERiPTHR24006:SF360. PTHR24006:SF360. 1 hit.
PfamiPF06337. DUSP. 1 hit.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 1 hit.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 1 hit.
PROSITEiPS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2GUZ1-1 [UniParc]FASTAAdd to Basket

« Hide

MAEGRGTHER PDVETQKTEL GALMGTTLQR GAQWYLIDSR WFKQWKKYVG    50
FDSWDMYNVG EHNLFPGPID NSGLFSDPES QTLKEHLIDE LDYVLVPTEA 100
WNKLLNWYGC VEGQQPIVRK VVEHGLFVKH CKVEVYLLEL KLCENSDPTN 150
VLSCHFSKAD TIATIEKEMR KLFNIPAERE TRLWNKYMSN TYEQLSKLDN 200
TIQDAGLYQG QVLVIEPQNE DGTWPRQTLQ SKSSTAPSRN FTTSSKPSAS 250
PYSSMSASLI ANGDSTNSSG MHNSGVSRGG AGFSASYNCQ EPPSPHIQPG 300
LCGLGNLGNT CFMNSALQCL SNTGPLTEYF LKDEYEAEIN RDNPLGMKGE 350
IAEAYAELIK QMWSGRDTHV APRMFKTQVG RFAPQFSGYQ QQDSQELLAF 400
ILDGLHEDLN RVKKKPYLEP KDANGRPDAV VAKEAWENHR LRNDSVIVDT 450
FHGLFKSTLV CPECAKVSVT FDPFCYLTLP LPLKKDRIME VFLVPADPHC 500
RPIQYRVTVP LMGAISDLCE ALSKLSGIAA ENMVVTDVYN HRFHKIFQMD 550
EGLSHITPRD DIFVYEICTT PMDGSEYITL PVYFREKKSR PSSTSSGAVL 600
YGQPLLVSVP KHRLTLESLY QAVCERISRY IKQPLPEEFL SSPLEPGACN 650
GSRGSYEGDE EEMDHQEEGK EQLSEVEESG EDSQGGDPTE TTQKAKGPPR 700
HKRLFTFSLV NSCGTADINS LATDGKLLKL NSRSTLAIDW DSETRSLYFD 750
EQESEACEKH TSMSQPQKKK KAAIALRECI ELFTTMETLG EHDPWYCPTC 800
KKHQQATKKF DLWSLPKILV VHLKRFSYNR YWRDKLDTVV EFPVRALNMS 850
EFVCDRAARP YVYDLIAVSN HYGAMGVGHY TAYAKNRLNG KWYYFDDSSV 900
SLASEDQIVT KAAYVLFYQR RDDECPSTSS PVSFPGSDGG AKLSSSQQDL 950
GEEEAYTMDT N 961
Length:961
Mass (Da):108,373
Last modified:June 10, 2008 - v1
Checksum:iD6DD975327451B9F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC166460 mRNA. Translation: AAI66460.1.
CH473954 Genomic DNA. Translation: EDL77177.1.
RefSeqiNP_001128484.1. NM_001135012.1.
XP_006243773.1. XM_006243711.1.
UniGeneiRn.92126.

Genome annotation databases

EnsembliENSRNOT00000073725; ENSRNOP00000066449; ENSRNOG00000046486.
GeneIDi290864.
KEGGirno:290864.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC166460 mRNA. Translation: AAI66460.1 .
CH473954 Genomic DNA. Translation: EDL77177.1 .
RefSeqi NP_001128484.1. NM_001135012.1.
XP_006243773.1. XM_006243711.1.
UniGenei Rn.92126.

3D structure databases

ProteinModelPortali B2GUZ1.
SMRi B2GUZ1. Positions 1-124.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 253348. 1 interaction.

PTM databases

PhosphoSitei B2GUZ1.

Proteomic databases

PRIDEi B2GUZ1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000073725 ; ENSRNOP00000066449 ; ENSRNOG00000046486 .
GeneIDi 290864.
KEGGi rno:290864.

Organism-specific databases

CTDi 7375.
RGDi 1587387. Usp4.

Phylogenomic databases

GeneTreei ENSGT00670000097750.
HOVERGENi HBG000864.
KOi K11835.
OMAi VEFPTRG.
OrthoDBi EOG77Q4VW.
PhylomeDBi B2GUZ1.

Miscellaneous databases

NextBioi 631779.

Gene expression databases

Genevestigatori B2GUZ1.

Family and domain databases

Gene3Di 3.30.2230.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028135. Ub_USP-typ.
IPR028889. UCH/PAN2.
IPR028134. USP.
[Graphical view ]
PANTHERi PTHR24006:SF360. PTHR24006:SF360. 1 hit.
Pfami PF06337. DUSP. 1 hit.
PF14836. Ubiquitin_3. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 1 hit.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 1 hit.
PROSITEi PS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "The ubiquitin-specific protease Usp4 regulates the cell surface level of the A2A receptor."
    Milojevic T., Reiterer V., Stefan E., Korkhov V.M., Dorostkar M.M., Ducza E., Ogris E., Boehm S., Freissmuth M., Nanoff C.
    Mol. Pharmacol. 69:1083-1094(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiUBP4_RAT
AccessioniPrimary (citable) accession number: B2GUZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: June 10, 2008
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi