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Protein

Serine/threonine-protein kinase PLK4

Gene

Plk4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. Required for the recruitment of STIL to the centriole and for STIL-mediated centriole amplification (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-2565942 Regulation of PLK1 Activity at G2/M Transition
R-RNO-380259 Loss of Nlp from mitotic centrosomes
R-RNO-380270 Recruitment of mitotic centrosome proteins and complexes
R-RNO-380320 Recruitment of NuMA to mitotic centrosomes
R-RNO-5620912 Anchoring of the basal body to the plasma membrane
R-RNO-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PLK4 (EC:2.7.11.21)
Alternative name(s):
Polo-like kinase 4
Short name:
PLK-4
Serine/threonine-protein kinase Sak
Gene namesi
Name:Plk4
Synonyms:Sak
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1305390 Plk4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003852811 – 924Serine/threonine-protein kinase PLK4Add BLAST924

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45N6-acetyllysineBy similarity1
Modified residuei46N6-acetyllysineBy similarity1
Modified residuei401PhosphoserineBy similarity1
Modified residuei777PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated; leading to its degradation by the proteasome.By similarity
Acetylation by KAT2A and KAT2B impairs kinase activity by shifting the kinase to an inactive conformation.By similarity
Tyrosine-phosphorylated by TEC.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiB2GUY1

Expressioni

Gene expression databases

BgeeiENSRNOG00000011654
ExpressionAtlasiB2GUY1 baseline and differential
GenevisibleiB2GUY1 RN

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with CEP152 (via N-terminus) (By similarity). Interacts with CEP78; this interaction may be important for proper PLK4 localization to the centriole and PLK4-induced overduplication of centrioles (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016946

Structurei

3D structure databases

ProteinModelPortaliB2GUY1
SMRiB2GUY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 265Protein kinasePROSITE-ProRule annotationAdd BLAST254
Domaini846 – 910POLO boxPROSITE-ProRule annotationAdd BLAST65

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0575 Eukaryota
ENOG410XQBP LUCA
GeneTreeiENSGT00530000062954
HOGENOMiHOG000237347
InParanoidiB2GUY1
OMAiGADFEVW
OrthoDBiEOG091G05NQ
PhylomeDBiB2GUY1

Family and domain databases

CDDicd13114 POLO_box_Plk4_1, 1 hit
cd13115 POLO_box_Plk4_2, 1 hit
cd13116 POLO_box_Plk4_3, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR033700 Plk4
IPR000959 POLO_box_dom
IPR033699 POLO_box_Plk4_1
IPR033698 POLO_box_Plk4_2
IPR033696 POLO_box_Plk4_C
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008266 Tyr_kinase_AS
PANTHERiPTHR24345:SF50 PTHR24345:SF50, 1 hit
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PF00659 POLO_box, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50078 POLO_BOX, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

B2GUY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAACIGERIE DFKVGNLLGK GSFAGVYRAE SIHTGLEVAI KMIDKKAMYK
60 70 80 90 100
AGMVQRVQNE VKIHCQLKHP SVLELYNYFE DNNYVYLVLE MCHNGEMNRY
110 120 130 140 150
LKNRMKPFSE SEARHFMHQI ITGMLYLHSH GILHRDLTLS NILLTRNMNI
160 170 180 190 200
KIADFGLATQ LKMPHEKHYT LCGTPNYISP EIATRSAHGL ESDIWSLGCM
210 220 230 240 250
FYTLLIGRPP FDTDTVKNTL NKVVLADYEM PAFLSREAQD LIHQLLRRNP
260 270 280 290 300
ADRLSLSSVL DHPFMSRNPS TKSKDLGTVE DSMDSGHATL STTITASSGT
310 320 330 340 350
SLSGSLLDRR RLLVGQPLPN KITVFQKNKN SSDFSSGDGS NFCTQWGNPE
360 370 380 390 400
QEANNRGRGR VIEDAEERPH SRYLRRAHSS DRSNPSNQSR AKTYSIERCH
410 420 430 440 450
SVEMLSKPRR SSLDETKHSS NHHCLGKTPF PFADQTPQME IVQQWFGNLQ
460 470 480 490 500
MNGETSEHNT ISPNRDFQDY PDVQDTLRNT WTDTRASKNS DNSANVHPAK
510 520 530 540 550
QLSTMKYMTA HHHKPEIMQQ ELAIHPHSEQ NKSRSMESTL GYRKPTLRSI
560 570 580 590 600
TSPLVAHRLK PIRQKTKKAV VSILDSEEVC VELLKECTSE GHVKEVLQIS
610 620 630 640 650
SDGTTITVYY PNDGRGFPLA DRPPLPTDNI SRYSFDSLPE KYWRKYQYAS
660 670 680 690 700
RFIQLVRSKT PKITYFTRYA KCILMENSPG ADFEVWFYDG AKIHKTEDVI
710 720 730 740 750
HIIEKTGLSY TLKNENDFTS LKEEVKIYMD HANEGHRTCL ALESVISEEE
760 770 780 790 800
KRSRGSSFFP IIVGRKPGTT SSPKALSPPP VDPGYSKGEQ ASSSRLSANS
810 820 830 840 850
AAFPTQTPVL SPSAVTVEGP GQTAATTGTS ISSSLPKSAQ LLKSVFVKNV
860 870 880 890 900
GWATQLTSGA VWVQFNDGSQ LVVQAGVSSI SYTSPDGQTT RYGENEKLPE
910 920
YIKQKLQCLS SILLMFSNPT PSFQ
Length:924
Mass (Da):103,770
Last modified:June 10, 2008 - v1
Checksum:iF79ACD48E54412F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166450 mRNA Translation: AAI66450.1
RefSeqiXP_006232361.1, XM_006232299.3
UniGeneiRn.14848

Genome annotation databases

EnsembliENSRNOT00000016946; ENSRNOP00000016946; ENSRNOG00000011654
GeneIDi310344

Similar proteinsi

Entry informationi

Entry nameiPLK4_RAT
AccessioniPrimary (citable) accession number: B2GUY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 10, 2008
Last modified: May 23, 2018
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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