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Protein

Serine/threonine-protein kinase PLK4

Gene

Plk4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/ (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATPPROSITE-ProRule annotation
Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-380259. Loss of Nlp from mitotic centrosomes.
R-RNO-380270. Recruitment of mitotic centrosome proteins and complexes.
R-RNO-5620912. Anchoring of the basal body to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PLK4 (EC:2.7.11.21)
Alternative name(s):
Polo-like kinase 4
Short name:
PLK-4
Serine/threonine-protein kinase Sak
Gene namesi
Name:Plk4
Synonyms:Sak
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1305390. Plk4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 924924Serine/threonine-protein kinase PLK4PRO_0000385281Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei401 – 4011PhosphoserineBy similarity
Modified residuei777 – 7771PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated; leading to its degradation by the proteasome.By similarity
Tyrosine-phosphorylated by TEC.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiB2GUY1.

Expressioni

Gene expression databases

ExpressionAtlasiB2GUY1. baseline and differential.
GenevisibleiB2GUY1. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with CEP152 (via N-terminus).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016946.

Structurei

3D structure databases

ProteinModelPortaliB2GUY1.
SMRiB2GUY1. Positions 1-266, 844-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 265254Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini846 – 91065POLO boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.PROSITE-ProRule annotation
Contains 1 POLO box domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0575. Eukaryota.
ENOG410XQBP. LUCA.
GeneTreeiENSGT00530000062954.
HOGENOMiHOG000237347.
InParanoidiB2GUY1.
OMAiTYTMERC.
OrthoDBiEOG7NCV33.
PhylomeDBiB2GUY1.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000959. POLO_box_duplicated_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00659. POLO_box. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50078. POLO_BOX. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2GUY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAACIGERIE DFKVGNLLGK GSFAGVYRAE SIHTGLEVAI KMIDKKAMYK
60 70 80 90 100
AGMVQRVQNE VKIHCQLKHP SVLELYNYFE DNNYVYLVLE MCHNGEMNRY
110 120 130 140 150
LKNRMKPFSE SEARHFMHQI ITGMLYLHSH GILHRDLTLS NILLTRNMNI
160 170 180 190 200
KIADFGLATQ LKMPHEKHYT LCGTPNYISP EIATRSAHGL ESDIWSLGCM
210 220 230 240 250
FYTLLIGRPP FDTDTVKNTL NKVVLADYEM PAFLSREAQD LIHQLLRRNP
260 270 280 290 300
ADRLSLSSVL DHPFMSRNPS TKSKDLGTVE DSMDSGHATL STTITASSGT
310 320 330 340 350
SLSGSLLDRR RLLVGQPLPN KITVFQKNKN SSDFSSGDGS NFCTQWGNPE
360 370 380 390 400
QEANNRGRGR VIEDAEERPH SRYLRRAHSS DRSNPSNQSR AKTYSIERCH
410 420 430 440 450
SVEMLSKPRR SSLDETKHSS NHHCLGKTPF PFADQTPQME IVQQWFGNLQ
460 470 480 490 500
MNGETSEHNT ISPNRDFQDY PDVQDTLRNT WTDTRASKNS DNSANVHPAK
510 520 530 540 550
QLSTMKYMTA HHHKPEIMQQ ELAIHPHSEQ NKSRSMESTL GYRKPTLRSI
560 570 580 590 600
TSPLVAHRLK PIRQKTKKAV VSILDSEEVC VELLKECTSE GHVKEVLQIS
610 620 630 640 650
SDGTTITVYY PNDGRGFPLA DRPPLPTDNI SRYSFDSLPE KYWRKYQYAS
660 670 680 690 700
RFIQLVRSKT PKITYFTRYA KCILMENSPG ADFEVWFYDG AKIHKTEDVI
710 720 730 740 750
HIIEKTGLSY TLKNENDFTS LKEEVKIYMD HANEGHRTCL ALESVISEEE
760 770 780 790 800
KRSRGSSFFP IIVGRKPGTT SSPKALSPPP VDPGYSKGEQ ASSSRLSANS
810 820 830 840 850
AAFPTQTPVL SPSAVTVEGP GQTAATTGTS ISSSLPKSAQ LLKSVFVKNV
860 870 880 890 900
GWATQLTSGA VWVQFNDGSQ LVVQAGVSSI SYTSPDGQTT RYGENEKLPE
910 920
YIKQKLQCLS SILLMFSNPT PSFQ
Length:924
Mass (Da):103,770
Last modified:June 10, 2008 - v1
Checksum:iF79ACD48E54412F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166450 mRNA. Translation: AAI66450.1.
RefSeqiXP_006232361.1. XM_006232299.2.
UniGeneiRn.14848.

Genome annotation databases

EnsembliENSRNOT00000016946; ENSRNOP00000016946; ENSRNOG00000011654.
GeneIDi310344.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166450 mRNA. Translation: AAI66450.1.
RefSeqiXP_006232361.1. XM_006232299.2.
UniGeneiRn.14848.

3D structure databases

ProteinModelPortaliB2GUY1.
SMRiB2GUY1. Positions 1-266, 844-918.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016946.

Proteomic databases

PaxDbiB2GUY1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016946; ENSRNOP00000016946; ENSRNOG00000011654.
GeneIDi310344.

Organism-specific databases

CTDi10733.
RGDi1305390. Plk4.

Phylogenomic databases

eggNOGiKOG0575. Eukaryota.
ENOG410XQBP. LUCA.
GeneTreeiENSGT00530000062954.
HOGENOMiHOG000237347.
InParanoidiB2GUY1.
OMAiTYTMERC.
OrthoDBiEOG7NCV33.
PhylomeDBiB2GUY1.

Enzyme and pathway databases

ReactomeiR-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-380259. Loss of Nlp from mitotic centrosomes.
R-RNO-380270. Recruitment of mitotic centrosome proteins and complexes.
R-RNO-5620912. Anchoring of the basal body to the plasma membrane.

Miscellaneous databases

PROiB2GUY1.

Gene expression databases

ExpressionAtlasiB2GUY1. baseline and differential.
GenevisibleiB2GUY1. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000959. POLO_box_duplicated_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00659. POLO_box. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50078. POLO_BOX. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiPLK4_RAT
AccessioniPrimary (citable) accession number: B2GUY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 10, 2008
Last modified: May 11, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.