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Protein

Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

Gene

Man1b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2 (By similarity).By similarity

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei288Proton donorBy similarity1
Active sitei421By similarity1
Active sitei528Proton donorBy similarity1
Active sitei557By similarity1
Metal bindingi646CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-901032 ER Quality Control Compartment (ERQC)
UniPathwayiUPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC:3.2.1.113By similarity)
Alternative name(s):
ER alpha-1,2-mannosidase
ER mannosidase 1
Short name:
ERMan1
Mannosidase alpha class 1B member 1
Gene namesi
Name:Man1b1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1563595 Man1b1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 49CytoplasmicSequence analysisAdd BLAST49
Transmembranei50 – 70HelicalSequence analysisAdd BLAST21
Topological domaini71 – 657LumenalSequence analysisAdd BLAST587

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003966231 – 657Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidaseAdd BLAST657

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101PhosphoserineBy similarity1
Disulfide bondi485 ↔ 514By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiB2GUY0
PeptideAtlasiB2GUY0
PRIDEiB2GUY0

Expressioni

Gene expression databases

BgeeiENSRNOG00000012559
GenevisibleiB2GUY0 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016846

Structurei

3D structure databases

ProteinModelPortaliB2GUY0
SMRiB2GUY0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 10Poly-Pro8
Compositional biasi31 – 34Poly-Arg4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
GeneTreeiENSGT00390000016529
HOGENOMiHOG000181987
HOVERGENiHBG052389
InParanoidiB2GUY0
KOiK01230
OMAiDHLTCYL
OrthoDBiEOG091G0342
PhylomeDBiB2GUY0
TreeFamiTF354274

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequencei

Sequence statusi: Complete.

B2GUY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPPPPAPAP HRDFISVTLS LGESYDNSKS RRRRSCWRKW KQLSRLQRNV
60 70 80 90 100
ILFVLGFLIL CGFLYSLQVS DQWKALSGSR AEVEKMKLEV LPVLPAPQKE
110 120 130 140 150
SAEPEGFADI LSQKRQRHLR RGPPHLQIRP PNTVSKDGMQ DDAKEREAAL
160 170 180 190 200
GKAQQEENTQ RTVISWRGAV IEPEQATEPP SKRAEASIKP LFLASRIWKE
210 220 230 240 250
PAPPNERQKG VIEAFLHAWK GYQKFAWGHD ELKPVSKTFS EWFGLGLTLI
260 270 280 290 300
DALDTMWILG LKQEFKEARK WVSENLDFQK NVDVNLFEST IRILGGLLSA
310 320 330 340 350
YHLSGDSLFL SKAEDFGNRL MPAFTTPSKI PYSDVNIGTG FAHSPQWTSD
360 370 380 390 400
STVAEVTSIQ LEFRELSRLT GIKKFQEAVE EVTKHIHSLS GKKDGLVPMF
410 420 430 440 450
INTNSGLFTH PGVFTLGARA DSYYEYLLKQ WIQGGKKETQ LLEDYVRAIE
460 470 480 490 500
GIKAHLLRQS QPRKLTFVGE LAHGRFSAKM DHLVCFLPGT LALGVHHGLP
510 520 530 540 550
ADHMDLARAL METCYQMNQQ METGLSPEIA HFNMYPRADH KDVEVKPADR
560 570 580 590 600
HNLLRPETVE SLFYLYRVTK DRKYQDWGWE ILQSFNKYTR VPSGGYSSIN
610 620 630 640 650
NVQNSHKPEP RDKMESFFVG ETLKYLYLLF SDDLELLGLD TCVFNTEAHP

LPIWSPA
Length:657
Mass (Da):74,895
Last modified:August 10, 2010 - v2
Checksum:iA846B228A461367C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166449 mRNA Translation: AAI66449.1
RefSeqiNP_001102666.1, NM_001109196.1
UniGeneiRn.86273

Genome annotation databases

EnsembliENSRNOT00000016846; ENSRNOP00000016846; ENSRNOG00000012559
GeneIDi499751
KEGGirno:499751
UCSCiRGD:1563595 rat

Similar proteinsi

Entry informationi

Entry nameiMA1B1_RAT
AccessioniPrimary (citable) accession number: B2GUY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: March 28, 2018
This is version 67 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health