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B2GUY0

- MA1B1_RAT

UniProt

B2GUY0 - MA1B1_RAT

Protein

Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

Gene

Man1b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 2 (10 Aug 2010)
      Previous versions | rss
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    Functioni

    Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2 By similarity.By similarity

    Catalytic activityi

    Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

    Cofactori

    Calcium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei288 – 2881Proton donorBy similarity
    Active sitei421 – 4211By similarity
    Active sitei557 – 5571By similarity

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: Ensembl
    2. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_196403. ER Quality Control Compartment (ERQC).
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGH47. Glycoside Hydrolase Family 47.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC:3.2.1.113)
    Alternative name(s):
    ER alpha-1,2-mannosidase
    ER mannosidase 1
    Short name:
    ERMan1
    Mannosidase alpha class 1B member 1
    Gene namesi
    Name:Man1b1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi1563595. Man1b1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidasePRO_0000396623Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi485 ↔ 514By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiB2GUY0.

    Expressioni

    Gene expression databases

    GenevestigatoriB2GUY0.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000016846.

    Structurei

    3D structure databases

    ProteinModelPortaliB2GUY0.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4949CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini71 – 657587LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei50 – 7021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi3 – 108Poly-Pro
    Compositional biasi31 – 344Poly-Arg

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300315.
    GeneTreeiENSGT00390000016529.
    HOGENOMiHOG000181987.
    HOVERGENiHBG052389.
    KOiK01230.
    OMAiVHFNLHA.
    OrthoDBiEOG7ZGX2S.
    PhylomeDBiB2GUY0.
    TreeFamiTF354274.

    Family and domain databases

    Gene3Di1.50.10.50. 1 hit.
    InterProiIPR001382. Glyco_hydro_47.
    [Graphical view]
    PANTHERiPTHR11742. PTHR11742. 1 hit.
    PfamiPF01532. Glyco_hydro_47. 1 hit.
    [Graphical view]
    PRINTSiPR00747. GLYHDRLASE47.
    SUPFAMiSSF48225. SSF48225. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B2GUY0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYPPPPAPAP HRDFISVTLS LGESYDNSKS RRRRSCWRKW KQLSRLQRNV    50
    ILFVLGFLIL CGFLYSLQVS DQWKALSGSR AEVEKMKLEV LPVLPAPQKE 100
    SAEPEGFADI LSQKRQRHLR RGPPHLQIRP PNTVSKDGMQ DDAKEREAAL 150
    GKAQQEENTQ RTVISWRGAV IEPEQATEPP SKRAEASIKP LFLASRIWKE 200
    PAPPNERQKG VIEAFLHAWK GYQKFAWGHD ELKPVSKTFS EWFGLGLTLI 250
    DALDTMWILG LKQEFKEARK WVSENLDFQK NVDVNLFEST IRILGGLLSA 300
    YHLSGDSLFL SKAEDFGNRL MPAFTTPSKI PYSDVNIGTG FAHSPQWTSD 350
    STVAEVTSIQ LEFRELSRLT GIKKFQEAVE EVTKHIHSLS GKKDGLVPMF 400
    INTNSGLFTH PGVFTLGARA DSYYEYLLKQ WIQGGKKETQ LLEDYVRAIE 450
    GIKAHLLRQS QPRKLTFVGE LAHGRFSAKM DHLVCFLPGT LALGVHHGLP 500
    ADHMDLARAL METCYQMNQQ METGLSPEIA HFNMYPRADH KDVEVKPADR 550
    HNLLRPETVE SLFYLYRVTK DRKYQDWGWE ILQSFNKYTR VPSGGYSSIN 600
    NVQNSHKPEP RDKMESFFVG ETLKYLYLLF SDDLELLGLD TCVFNTEAHP 650
    LPIWSPA 657
    Length:657
    Mass (Da):74,895
    Last modified:August 10, 2010 - v2
    Checksum:iA846B228A461367C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC166449 mRNA. Translation: AAI66449.1.
    RefSeqiNP_001102666.1. NM_001109196.1.
    UniGeneiRn.86273.

    Genome annotation databases

    EnsembliENSRNOT00000016846; ENSRNOP00000016846; ENSRNOG00000012559.
    GeneIDi499751.
    KEGGirno:499751.
    UCSCiRGD:1563595. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC166449 mRNA. Translation: AAI66449.1 .
    RefSeqi NP_001102666.1. NM_001109196.1.
    UniGenei Rn.86273.

    3D structure databases

    ProteinModelPortali B2GUY0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000016846.

    Protein family/group databases

    CAZyi GH47. Glycoside Hydrolase Family 47.

    Proteomic databases

    PaxDbi B2GUY0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000016846 ; ENSRNOP00000016846 ; ENSRNOG00000012559 .
    GeneIDi 499751.
    KEGGi rno:499751.
    UCSCi RGD:1563595. rat.

    Organism-specific databases

    CTDi 11253.
    RGDi 1563595. Man1b1.

    Phylogenomic databases

    eggNOGi NOG300315.
    GeneTreei ENSGT00390000016529.
    HOGENOMi HOG000181987.
    HOVERGENi HBG052389.
    KOi K01230.
    OMAi VHFNLHA.
    OrthoDBi EOG7ZGX2S.
    PhylomeDBi B2GUY0.
    TreeFami TF354274.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196403. ER Quality Control Compartment (ERQC).

    Miscellaneous databases

    NextBioi 703875.
    PROi B2GUY0.

    Gene expression databases

    Genevestigatori B2GUY0.

    Family and domain databases

    Gene3Di 1.50.10.50. 1 hit.
    InterProi IPR001382. Glyco_hydro_47.
    [Graphical view ]
    PANTHERi PTHR11742. PTHR11742. 1 hit.
    Pfami PF01532. Glyco_hydro_47. 1 hit.
    [Graphical view ]
    PRINTSi PR00747. GLYHDRLASE47.
    SUPFAMi SSF48225. SSF48225. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiMA1B1_RAT
    AccessioniPrimary (citable) accession number: B2GUY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 10, 2010
    Last sequence update: August 10, 2010
    Last modified: October 1, 2014
    This is version 41 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3