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Protein

Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase

Gene

Man1b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2 (By similarity).By similarity

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactori

Ca2+By similarity

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei288 – 2881Proton donorBy similarity
Active sitei421 – 4211By similarity
Active sitei557 – 5571By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_338258. ER Quality Control Compartment (ERQC).
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC:3.2.1.113)
Alternative name(s):
ER alpha-1,2-mannosidase
ER mannosidase 1
Short name:
ERMan1
Mannosidase alpha class 1B member 1
Gene namesi
Name:Man1b1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1563595. Man1b1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4949CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei50 – 7021HelicalSequence AnalysisAdd
BLAST
Topological domaini71 – 657587LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidasePRO_0000396623Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi485 ↔ 514By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiB2GUY0.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016846.

Structurei

3D structure databases

ProteinModelPortaliB2GUY0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 108Poly-Pro
Compositional biasi31 – 344Poly-Arg

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300315.
GeneTreeiENSGT00390000016529.
HOGENOMiHOG000181987.
HOVERGENiHBG052389.
InParanoidiB2GUY0.
KOiK01230.
OMAiKEFAWGH.
OrthoDBiEOG7ZGX2S.
PhylomeDBiB2GUY0.
TreeFamiTF354274.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.

Sequencei

Sequence statusi: Complete.

B2GUY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYPPPPAPAP HRDFISVTLS LGESYDNSKS RRRRSCWRKW KQLSRLQRNV
60 70 80 90 100
ILFVLGFLIL CGFLYSLQVS DQWKALSGSR AEVEKMKLEV LPVLPAPQKE
110 120 130 140 150
SAEPEGFADI LSQKRQRHLR RGPPHLQIRP PNTVSKDGMQ DDAKEREAAL
160 170 180 190 200
GKAQQEENTQ RTVISWRGAV IEPEQATEPP SKRAEASIKP LFLASRIWKE
210 220 230 240 250
PAPPNERQKG VIEAFLHAWK GYQKFAWGHD ELKPVSKTFS EWFGLGLTLI
260 270 280 290 300
DALDTMWILG LKQEFKEARK WVSENLDFQK NVDVNLFEST IRILGGLLSA
310 320 330 340 350
YHLSGDSLFL SKAEDFGNRL MPAFTTPSKI PYSDVNIGTG FAHSPQWTSD
360 370 380 390 400
STVAEVTSIQ LEFRELSRLT GIKKFQEAVE EVTKHIHSLS GKKDGLVPMF
410 420 430 440 450
INTNSGLFTH PGVFTLGARA DSYYEYLLKQ WIQGGKKETQ LLEDYVRAIE
460 470 480 490 500
GIKAHLLRQS QPRKLTFVGE LAHGRFSAKM DHLVCFLPGT LALGVHHGLP
510 520 530 540 550
ADHMDLARAL METCYQMNQQ METGLSPEIA HFNMYPRADH KDVEVKPADR
560 570 580 590 600
HNLLRPETVE SLFYLYRVTK DRKYQDWGWE ILQSFNKYTR VPSGGYSSIN
610 620 630 640 650
NVQNSHKPEP RDKMESFFVG ETLKYLYLLF SDDLELLGLD TCVFNTEAHP

LPIWSPA
Length:657
Mass (Da):74,895
Last modified:August 10, 2010 - v2
Checksum:iA846B228A461367C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166449 mRNA. Translation: AAI66449.1.
RefSeqiNP_001102666.1. NM_001109196.1.
UniGeneiRn.86273.

Genome annotation databases

EnsembliENSRNOT00000016846; ENSRNOP00000016846; ENSRNOG00000012559.
GeneIDi499751.
KEGGirno:499751.
UCSCiRGD:1563595. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166449 mRNA. Translation: AAI66449.1.
RefSeqiNP_001102666.1. NM_001109196.1.
UniGeneiRn.86273.

3D structure databases

ProteinModelPortaliB2GUY0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016846.

Protein family/group databases

CAZyiGH47. Glycoside Hydrolase Family 47.

Proteomic databases

PaxDbiB2GUY0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016846; ENSRNOP00000016846; ENSRNOG00000012559.
GeneIDi499751.
KEGGirno:499751.
UCSCiRGD:1563595. rat.

Organism-specific databases

CTDi11253.
RGDi1563595. Man1b1.

Phylogenomic databases

eggNOGiNOG300315.
GeneTreeiENSGT00390000016529.
HOGENOMiHOG000181987.
HOVERGENiHBG052389.
InParanoidiB2GUY0.
KOiK01230.
OMAiKEFAWGH.
OrthoDBiEOG7ZGX2S.
PhylomeDBiB2GUY0.
TreeFamiTF354274.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_338258. ER Quality Control Compartment (ERQC).

Miscellaneous databases

NextBioi703875.
PROiB2GUY0.

Family and domain databases

Gene3Di1.50.10.50. 1 hit.
InterProiIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERiPTHR11742. PTHR11742. 1 hit.
PfamiPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSiPR00747. GLYHDRLASE47.
SUPFAMiSSF48225. SSF48225. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiMA1B1_RAT
AccessioniPrimary (citable) accession number: B2GUY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: June 24, 2015
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.