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B2GUY0 (MA1B1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
EC=3.2.1.113
Alternative name(s):
ER alpha-1,2-mannosidase
ER mannosidase 1
Short name=ERMan1
Mannosidase alpha class 1B member 1
Gene names
Name:Man1b1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length657 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man9GlcNAc2 to produce Man8GlcNAc2, but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man5-6GlcNAc2 By similarity.

Catalytic activity

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

Cofactor

Calcium By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein.

Sequence similarities

Belongs to the glycosyl hydrolase 47 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 657657Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
PRO_0000396623

Regions

Topological domain1 – 4949Cytoplasmic Potential
Transmembrane50 – 7021Helical; Potential
Topological domain71 – 657587Lumenal Potential
Compositional bias3 – 108Poly-Pro
Compositional bias31 – 344Poly-Arg

Sites

Active site2881Proton donor By similarity
Active site4211 By similarity
Active site5571 By similarity

Amino acid modifications

Disulfide bond485 ↔ 514 By similarity

Sequences

Sequence LengthMass (Da)Tools
B2GUY0 [UniParc].

Last modified August 10, 2010. Version 2.
Checksum: A846B228A461367C

FASTA65774,895
        10         20         30         40         50         60 
MYPPPPAPAP HRDFISVTLS LGESYDNSKS RRRRSCWRKW KQLSRLQRNV ILFVLGFLIL 

        70         80         90        100        110        120 
CGFLYSLQVS DQWKALSGSR AEVEKMKLEV LPVLPAPQKE SAEPEGFADI LSQKRQRHLR 

       130        140        150        160        170        180 
RGPPHLQIRP PNTVSKDGMQ DDAKEREAAL GKAQQEENTQ RTVISWRGAV IEPEQATEPP 

       190        200        210        220        230        240 
SKRAEASIKP LFLASRIWKE PAPPNERQKG VIEAFLHAWK GYQKFAWGHD ELKPVSKTFS 

       250        260        270        280        290        300 
EWFGLGLTLI DALDTMWILG LKQEFKEARK WVSENLDFQK NVDVNLFEST IRILGGLLSA 

       310        320        330        340        350        360 
YHLSGDSLFL SKAEDFGNRL MPAFTTPSKI PYSDVNIGTG FAHSPQWTSD STVAEVTSIQ 

       370        380        390        400        410        420 
LEFRELSRLT GIKKFQEAVE EVTKHIHSLS GKKDGLVPMF INTNSGLFTH PGVFTLGARA 

       430        440        450        460        470        480 
DSYYEYLLKQ WIQGGKKETQ LLEDYVRAIE GIKAHLLRQS QPRKLTFVGE LAHGRFSAKM 

       490        500        510        520        530        540 
DHLVCFLPGT LALGVHHGLP ADHMDLARAL METCYQMNQQ METGLSPEIA HFNMYPRADH 

       550        560        570        580        590        600 
KDVEVKPADR HNLLRPETVE SLFYLYRVTK DRKYQDWGWE ILQSFNKYTR VPSGGYSSIN 

       610        620        630        640        650 
NVQNSHKPEP RDKMESFFVG ETLKYLYLLF SDDLELLGLD TCVFNTEAHP LPIWSPA

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC166449 mRNA. Translation: AAI66449.1.
IPIIPI00373467.
RefSeqNP_001102666.1. NM_001109196.1.
UniGeneRn.86273.

3D structure databases

ProteinModelPortalB2GUY0.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000016846.

Protein family/group databases

CAZyGH47. Glycoside Hydrolase Family 47.

Proteomic databases

PaxDbB2GUY0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000016846; ENSRNOP00000016846; ENSRNOG00000012559.
GeneID499751.
KEGGrno:499751.
UCSCRGD:1563595. rat.

Organism-specific databases

CTD11253.
RGD1563595. Man1b1.

Phylogenomic databases

eggNOGNOG300315.
GeneTreeENSGT00390000016529.
HOGENOMHOG000181987.
HOVERGENHBG052389.
KOK01230.
OMAHFNLHAQ.
OrthoDBEOG4J9MZB.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

ArrayExpressB2GUY0.
GenevestigatorB2GUY0.

Family and domain databases

Gene3D1.50.10.50. 1 hit.
InterProIPR001382. Glyco_hydro_47.
[Graphical view]
PANTHERPTHR11742. PTHR11742. 1 hit.
PfamPF01532. Glyco_hydro_47. 1 hit.
[Graphical view]
PRINTSPR00747. GLYHDRLASE47.
SUPFAMSSF48225. Glyco_hydro_47. 1 hit.
ProtoNetSearch...

Other

NextBio703875.

Entry information

Entry nameMA1B1_RAT
AccessionPrimary (citable) accession number: B2GUY0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: April 3, 2013
This is version 32 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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