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B2GUX4

- UBP21_RAT

UniProt

B2GUX4 - UBP21_RAT

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Protein

Ubiquitin carboxyl-terminal hydrolase 21

Gene

Usp21

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A releaves the repression of di- and trimethylation of histone H3 at 'Lys-4', resulting in regulation of transcriptional initiation. Regulates gene expression via histone H2A deubiquitination. Also capable of removing NEDD8 from NEDD8 conjugates but has no effect on Sentrin-1 conjugates (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei221 – 2211NucleophilePROSITE-ProRule annotation
Metal bindingi384 – 3841ZincBy similarity
Metal bindingi387 – 3871ZincBy similarity
Metal bindingi437 – 4371ZincBy similarity
Metal bindingi440 – 4401ZincBy similarity
Active sitei518 – 5181Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. NEDD8-specific protease activity Source: UniProtKB
  4. transcription coactivator activity Source: UniProtKB
  5. ubiquitin-specific protease activity Source: UniProtKB
  6. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. histone deubiquitination Source: UniProtKB
  2. positive regulation of transcription, DNA-templated Source: UniProtKB
  3. transcription, DNA-templated Source: UniProtKB-KW
  4. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 21 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 21
Ubiquitin thioesterase 21
Ubiquitin-specific-processing protease 21
Gene namesi
Name:Usp21
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 13

Organism-specific databases

RGDi1588822. Usp21.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
  3. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Ubiquitin carboxyl-terminal hydrolase 21PRO_0000367509Add
BLAST

Proteomic databases

PRIDEiB2GUX4.

Expressioni

Gene expression databases

GenevestigatoriB2GUX4.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000055192.

Structurei

3D structure databases

ProteinModelPortaliB2GUX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini212 – 558347USPAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi134 – 15219Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP21 subfamily.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00760000119208.
HOVERGENiHBG011164.
InParanoidiB2GUX4.
KOiK11833.
OMAiNVQPRVG.
OrthoDBiEOG7FR7GN.
PhylomeDBiB2GUX4.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2GUX4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPQASEHRLG RTREPPVNVQ PRVGAKIPFP PRARSKERRN PVPGPNSMLR
60 70 80 90 100
PLPPRPGPPD ERLKKLDLGR GRTSGSRPRG PLRADHGVPL PGSPPPTVAL
110 120 130 140 150
PLPSRTNLTR SKSVSSGDLR PMGIALGGHR GTGELGAALS RLALRPEPPT
160 170 180 190 200
LRRSTSLRRL GGFPGPPTLL SIRTEPPPSH GSFHMISARP SEPFYSDDKM
210 220 230 240 250
AHHTLLLGSG HVGLRNLGNT CFLNAVLQCL SSTRPLRDFC LRRDFRQEVP
260 270 280 290 300
GGGRAQELTE AFADVIGALW HPDSCEAVNP TRFRAVFQKY VPSFSGYSQQ
310 320 330 340 350
DAQEFLKLLM ERLHLEINRR GRRAPPILAS GPVPSPPRRG GALHEEPELS
360 370 380 390 400
DDDRANLMWK RYLEREDSKI VDLFVGQLKS CLKCQACGYR STTFEVFCDL
410 420 430 440 450
SLPIPKKGFA GGKVSLRDCF SLFTKEEELE SENAPVCDRC RQKTRSTKKL
460 470 480 490 500
TVQRFPRILV LHLNRFSTSR GSIKKSSVGV DFPLQRLSLG DFASDKVGSP
510 520 530 540 550
VYQLYALCNH SGSVHYGHYT ALCRCQTGWH VYNDSRVSPV SENQVASSEG
560
YVLFYQLMQE PPRCL
Length:565
Mass (Da):62,700
Last modified:June 10, 2008 - v1
Checksum:i37ABEBF9CE07E938
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC166443 mRNA. Translation: AAI66443.1.
RefSeqiNP_001121110.1. NM_001127638.1.
UniGeneiRn.136743.

Genome annotation databases

EnsembliENSRNOT00000058390; ENSRNOP00000055192; ENSRNOG00000038347.
GeneIDi688466.
KEGGirno:688466.
UCSCiRGD:1588822. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC166443 mRNA. Translation: AAI66443.1 .
RefSeqi NP_001121110.1. NM_001127638.1.
UniGenei Rn.136743.

3D structure databases

ProteinModelPortali B2GUX4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000055192.

Proteomic databases

PRIDEi B2GUX4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000058390 ; ENSRNOP00000055192 ; ENSRNOG00000038347 .
GeneIDi 688466.
KEGGi rno:688466.
UCSCi RGD:1588822. rat.

Organism-specific databases

CTDi 27005.
RGDi 1588822. Usp21.

Phylogenomic databases

eggNOGi COG5533.
GeneTreei ENSGT00760000119208.
HOVERGENi HBG011164.
InParanoidi B2GUX4.
KOi K11833.
OMAi NVQPRVG.
OrthoDBi EOG7FR7GN.
PhylomeDBi B2GUX4.

Miscellaneous databases

NextBioi 736555.

Gene expression databases

Genevestigatori B2GUX4.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiUBP21_RAT
AccessioniPrimary (citable) accession number: B2GUX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3