B2GLR9 (ILVD_KOCRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	KRH_02560

Organism

Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) [Complete proteome] [HAMAP]

Taxonomic identifier

378753 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Kocuria

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089392
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B2GLR9 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 7CB99F6FB1614500	FASTA	612	64,991
	10 20 30 40 50 60 MPQLRSATST TGRNMAGARA LWRATGMGSE DFGKPIVAIA NSFTQFVPGH VHLKNMGELV 70 80 90 100 110 120 ASAVQEAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRDII ADSVEYMVNA HCADALVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPV VFVSGGPMES GEGVEGVVEH RVDLVDAMSL AVDESVTDAQ 190 200 210 220 230 240 LAQIEENACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGT TLATHVNRKR LFLEAGRRAV 250 260 270 280 290 300 ESAKKYYEQD DESVLPRSIA TKAAFENAMA LDIAMGGSTN TVLHILAAAQ EAEVDFNLSD 310 320 330 340 350 360 IDALSRQVPC LAKVAPNSTT FHIEHVHRAG GIPAILGELR RGGLLNEDVH TVHSQTMGEW 370 380 390 400 410 420 LDEWDIRSGK ASDAAKEFFL AAPGGVRTTQ AFSQANEYED HDVDAAGGCI RSVEHAYTKE 430 440 450 460 470 480 GGLCVLFGNI AEDGAVIKTA GIDPELFHFT GRAFVVESQD EAVHEILSKN VKEGDIVVIA 490 500 510 520 530 540 YEGPKGGPGM QEMLYPTSYL KGLGLGKKCA LITDGRFSGG TSGLSIGHIS PEAAAGGAVG 550 560 570 580 590 600 LVQHGDEIEI DVENRVLRVN VDDAELARRR QEKGDAPWKP TKPRERRVSK ALKAYASMVT 610 SADKGAVRVV ED

References

[1]

"Complete genome sequence of the soil actinomycete Kocuria rhizophila." Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S., Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. J. Bacteriol. 190:4139-4146(2008) [PubMed: 18408034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009152 Genomic DNA. Translation: BAG28603.1.
RefSeq	YP_001854109.1. NC_010617.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2GLR9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6237777.
GenomeReviews	Gene locus KRH_02560 in contig AP009152_GR.
KEGG	krh:KRH_02560.
PATRIC	22180734. VBIKocRhi5258_0247.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	CLSK2801376.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_KOCRD

Accession

Primary (citable) accession number: B2GLR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program