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B2GLQ7 (BIOB_KOCRD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:KRH_02440
OrganismKocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) [Complete proteome] [HAMAP]
Taxonomic identifier378753 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeKocuria

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Biotin synthase HAMAP-Rule MF_01694
PRO_0000381436

Sites

Metal binding661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1431Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2031Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2731Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B2GLQ7 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: E398CDDA06A2FB43

FASTA46948,747
        10         20         30         40         50         60 
MFKNPAELAD RVLAGTPVTP DEALDLLRTD DPGMLDLVAA AARLRREHFG MTVKVNYLVN 

        70         80         90        100        110        120 
LKSGLCAEDC GYCSQRLNAG TDILKYSWLS PEQAVEQAEY GIKGGASRVC LVASGRGPSN 

       130        140        150        160        170        180 
REVGNVTRTV EALKEQHPDV EVCACLGLLK DGQAESLSSA GVNAYNHNLN TAESHYESIC 

       190        200        210        220        230        240 
STHTYQDRVD TVERAKDAGL SPCSGLIVGM RETDEQLVEA VFALRDLGSD SVPVNFLMPF 

       250        260        270        280        290        300 
EGTPLAGTWQ LSPQKCLKIL AVVRFVCPDK EIRMAGGREM HLRSLQPLAL HVVNSLFLGD 

       310        320        330        340        350        360 
YLTSEGQAAE ADLAMIADAG FTVLGAGPDP SRDRHAGPAD VQGSGAEQGA GERTTTPCGS 

       370        380        390        400        410        420 
VCGSAAGSSS GDGSAPDGGR APADVSRSGP AEAVGSSPED GSRNAGGPAR TRSAAASSAP 

       430        440        450        460 
TGAGMSPALA ALLGSDDGPE QDEDDTRVRV ELSPTVRRRG AGTSVAPNA 

« Hide

References

[1]"Complete genome sequence of the soil actinomycete Kocuria rhizophila."
Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S., Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
J. Bacteriol. 190:4139-4146(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009152 Genomic DNA. Translation: BAG28591.1.
RefSeqYP_001854097.1. NC_010617.1.

3D structure databases

ProteinModelPortalB2GLQ7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING378753.KRH_02440.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG28591; BAG28591; KRH_02440.
GeneID6237769.
KEGGkrh:KRH_02440.
PATRIC22180718. VBIKocRhi5258_0239.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMACTELATR.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycKRHI378753:GJ8F-249-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_KOCRD
AccessionPrimary (citable) accession number: B2GLQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways