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B2GLQ7

- BIOB_KOCRD

UniProt

B2GLQ7 - BIOB_KOCRD

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Protein

Biotin synthase

Gene
bioB, KRH_02440
Organism
Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi143 – 1431Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi203 – 2031Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi273 – 2731Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciKRHI378753:GJ8F-249-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:KRH_02440
OrganismiKocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201)
Taxonomic identifieri378753 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeKocuria
ProteomesiUP000008838: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Biotin synthaseUniRule annotationPRO_0000381436Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi378753.KRH_02440.

Structurei

3D structure databases

ProteinModelPortaliB2GLQ7.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiCTELATR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B2GLQ7-1 [UniParc]FASTAAdd to Basket

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MFKNPAELAD RVLAGTPVTP DEALDLLRTD DPGMLDLVAA AARLRREHFG    50
MTVKVNYLVN LKSGLCAEDC GYCSQRLNAG TDILKYSWLS PEQAVEQAEY 100
GIKGGASRVC LVASGRGPSN REVGNVTRTV EALKEQHPDV EVCACLGLLK 150
DGQAESLSSA GVNAYNHNLN TAESHYESIC STHTYQDRVD TVERAKDAGL 200
SPCSGLIVGM RETDEQLVEA VFALRDLGSD SVPVNFLMPF EGTPLAGTWQ 250
LSPQKCLKIL AVVRFVCPDK EIRMAGGREM HLRSLQPLAL HVVNSLFLGD 300
YLTSEGQAAE ADLAMIADAG FTVLGAGPDP SRDRHAGPAD VQGSGAEQGA 350
GERTTTPCGS VCGSAAGSSS GDGSAPDGGR APADVSRSGP AEAVGSSPED 400
GSRNAGGPAR TRSAAASSAP TGAGMSPALA ALLGSDDGPE QDEDDTRVRV 450
ELSPTVRRRG AGTSVAPNA 469
Length:469
Mass (Da):48,747
Last modified:June 10, 2008 - v1
Checksum:iE398CDDA06A2FB43
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009152 Genomic DNA. Translation: BAG28591.1.
RefSeqiYP_001854097.1. NC_010617.1.

Genome annotation databases

EnsemblBacteriaiBAG28591; BAG28591; KRH_02440.
GeneIDi6237769.
KEGGikrh:KRH_02440.
PATRICi22180718. VBIKocRhi5258_0239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009152 Genomic DNA. Translation: BAG28591.1 .
RefSeqi YP_001854097.1. NC_010617.1.

3D structure databases

ProteinModelPortali B2GLQ7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 378753.KRH_02440.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG28591 ; BAG28591 ; KRH_02440 .
GeneIDi 6237769.
KEGGi krh:KRH_02440.
PATRICi 22180718. VBIKocRhi5258_0239.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi CTELATR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci KRHI378753:GJ8F-249-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Entry informationi

Entry nameiBIOB_KOCRD
AccessioniPrimary (citable) accession number: B2GLQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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