B2GKS1 (SYP_KOCRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	KRH_16100

Organism

Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) [Complete proteome] [HAMAP]

Taxonomic identifier

378753 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Kocuria

Protein attributes

Sequence length	606 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 606	606	Proline--tRNA ligase HAMAP MF_01569		PRO_1000199398

Sequences

	Sequence		Length	Mass (Da)
	B2GKS1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: E45D57E72B54C22F	FASTA	606	65,715
	10 20 30 40 50 60 MPLRLSSLFL RTLREDPVDA DVDSHKLLVR AGYIRRAAPG IYTWLPLGLA VLRRVEGIVR 70 80 90 100 110 120 EEMDAIGAQE VHFPALLPRE PYEASNRWTE YGENLFRLQD RKGADYLLAP THEEMFTLLV 130 140 150 160 170 180 KDMYSSYKDL PVMLYQIQTK YRDEARPRAG LLRGREFIMK DSYSFDVDDA GLDDAYAKHR 190 200 210 220 230 240 AAYVRIFERL GLPVVAVSAT SGAMGGSRSE EFMHPSVVGE DTFVRSAGGY AANVEAVTTV 250 260 270 280 290 300 VPEPVDCTDA PAAQVHRTPD SPTIDTLVSR SNELHPREGQ PWTAADTLKN VLLSVSLPEG 310 320 330 340 350 360 GTQLVAVGVP GDREIDLKRV EAGIGGALGI GGELEVEAAS EEQLRAVPEL VKGYIGPGLS 370 380 390 400 410 420 REDALLGTES PTGIPYFVDP RVVPGTRWIT GANVAEAHVY DLVAERDFTW DATLEACTVR 430 440 450 460 470 480 EGDPAPDGSG PLEIARGMEM GHVFQLGRKY AEALGLKVLD NNGKLVTVTM GSYGIGVTRA 490 500 510 520 530 540 LAAVAEFYHD EHGLLWPRNL SPADVHVVAT GKGPEVLEAA EEIVAHLEAA GVSVLFDDRP 550 560 570 580 590 600 KVSPGVKFRD AELLGVPTVL VVGRGLADGV LELKDRRSGT SRDIARDRVV AEITAELQGQ EGPAAE

References

[1]

"Complete genome sequence of the soil actinomycete Kocuria rhizophila." Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S., Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. J. Bacteriol. 190:4139-4146(2008) [PubMed: 18408034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009152 Genomic DNA. Translation: BAG29957.1.
RefSeq	YP_001855463.1. NC_010617.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2GKS1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6238632.
GenomeReviews	Gene locus KRH_16100 in contig AP009152_GR.
KEGG	krh:KRH_16100.
PATRIC	22183520. VBIKocRhi5258_1607.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_KOCRD

Accession

Primary (citable) accession number: B2GKS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program