B2GKR5 (IF2_KOCRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-2

Gene names

Name:	infB
Ordered Locus Names:	KRH_16040

Organism

Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) [Complete proteome] [HAMAP]

Taxonomic identifier

378753 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Kocuria › Kocuria rhizophila

Protein attributes

Sequence length	967 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the IF-2 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 967	967	Translation initiation factor IF-2 HAMAP-Rule MF_00100		PRO_1000093795
Regions
	Nucleotide binding	469 – 476	8	GTP By similarity
	Nucleotide binding	519 – 523	5	GTP By similarity
	Nucleotide binding	573 – 576	4	GTP By similarity
	Region	463 – 615	153	G-domain HAMAP-Rule MF_00100

Sequences

	Sequence		Length	Mass (Da)
	B2GKR5 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 92F334A6CAF5FF93	FASTA	967	100,648
	10 20 30 40 50 60 MAKPRVHELA KELGITSKEA ISKLQELGEF VRGASSTVEP PVAKKLRTAF PAGGDSAPAA 70 80 90 100 110 120 KPAAKAPAQG VKKPAAAGPK PGAKAPAKPA ASGNAARAEK PAASEAPKAA AASSEAPKSA 130 140 150 160 170 180 APQQGTAPAA ASESSAPGTP ASSAPRPGGA TPGPRKSPAP GAKPGQSAPR PGNNPFASQQ 190 200 210 220 230 240 GMGRSEGGAQ RGGPRPGGQQ RSGKPGAPRP GNNPFAAQQG MRSANSGQGG PRPGGPRPGG 250 260 270 280 290 300 PRPAQSGQGG PRPGAPRTGA PRQGQGGPRP GGPRPSPNMM PGQTTSVAPI GSRPAPGSGP 310 320 330 340 350 360 RRGGGPGGGP GGGGGFRGRG GRGGTQGAFG RGGARGKHRK SKRAKRQELE QMSAPAVGGV 370 380 390 400 410 420 SVPHGDGSTV VRLRRGASLM DFAEKINANP ASLVTVLIHL GEMATQTQSL DEETFQLLGA 430 440 450 460 470 480 ELGYQIQVVS PEDEERELLE SFDIDLDAEL EAEGEDVLAP RPPVVTVMGH VDHGKTRLLD 490 500 510 520 530 540 AIRNTKVIEG EAGGITQHIG AYQISTEVDG QERLITFIDT PGHEAFTAMR ARGAQVTDIA 550 560 570 580 590 600 VLVVAADDGV MPQTVEALNH AQAANVPIVV AVNKVDKPDA NPDKIRGQLT EYGLVPEEYG 610 620 630 640 650 660 GDTMFVDVSA RENLNIDELL DAIVLTADGA LELQATPDKN ARGVAIEANL DKGRGAVCTV 670 680 690 700 710 720 LVQSGTLKVG DSIVAGAAYG RVRAMFDEMG RTVEAATPSR PVQVLGLSTV PRAGDTFLAT 730 740 750 760 770 780 QEERTARQIA EKREAADRNA QLAKRRKRIT LESFDEAVAE GKIDTLNLII KGDVSGAVEA 790 800 810 820 830 840 LEDSLLKIDV GEEVQLRVIH RGVGAITQND VNLATVDNAV IIGFNVRPAE RVTELADREG 850 860 870 880 890 900 VEMKFYSVIY NAIDEVESAL KGMLKPEYEE VELGTAEIRE VFRSSKWGNI AGAYVTKGLI 910 920 930 940 950 960 RRNGKARLVR DGNVVQDNLS IDSLRRFKDD ATEVREGYEC GIGLGSFNDI HEGDIIETWE MREKPRD

References

[1]

"Complete genome sequence of the soil actinomycete Kocuria rhizophila." Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S., Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. J. Bacteriol. 190:4139-4146(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009152 Genomic DNA. Translation: BAG29951.1.
RefSeq	YP_001855457.1. NC_010617.1.
3D structure databases
ProteinModelPortal	B2GKR5.
ModBase	Search...
Protein-protein interaction databases
STRING	378753.KRH_16040.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAG29951; BAG29951; KRH_16040.
GeneID	6239780.
KEGG	krh:KRH_16040.
PATRIC	22183508. VBIKocRhi5258_1601.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0532.
HOGENOM	HOG000076906.
KO	K02519.
OMA	PGNNPFA.
ProtClustDB	PRK05306.
Enzyme and pathway databases
BioCyc	KRHI378753:GJ8F-1644-MONOMER.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
HAMAP	MF_00100_B. IF-2_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR006847. IF2_N. IPR005225. Small_GTP-bd_dom. IPR000178. TF_IF2_bacterial-like. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. PF04760. IF2_N. 2 hits. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 2 hits.
TIGRFAMs	TIGR00487. IF-2. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF2_KOCRD

Accession

Primary (citable) accession number: B2GKR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program