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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi528 – 5281MagnesiumUniRule annotation
Metal bindingi534 – 5341MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciKRHI378753:GJ8F-1635-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:KRH_15950
OrganismiKocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201)
Taxonomic identifieri378753 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeKocuria
ProteomesiUP000008838 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 751751Polyribonucleotide nucleotidyltransferasePRO_1000147925Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi378753.KRH_15950.

Structurei

3D structure databases

ProteinModelPortaliB2GKI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini594 – 65360KHUniRule annotationAdd
BLAST
Domaini665 – 73773S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2GKI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGPEIQSAE AVIDNGSYGK RVVRFETGRL ARQAAGSAMV YIDEETSMLS
60 70 80 90 100
ATAVGKHPRE GFDFFPLTVD VEERMYAAGR IPGSFFRREG RPSTDAILTC
110 120 130 140 150
RLIDRPLRPA FGKGLRNEVQ VVVTVMSIAP DEIYNTVAIN AASMSTTLSG
160 170 180 190 200
MPFQGPVGGV RMALMAEENG GHQWIAFPKH SQLENAVFDM AVAGRVVTTK
210 220 230 240 250
DGGQDVAIMM VEAEATDNAW ELIKGQGAVA PSEELVAEGL EASKPFIKAL
260 270 280 290 300
CDAQADLAAR NRKPELDLPR FGGFGDDAAQ AVEQFVGERM AQVYSIAGKQ
310 320 330 340 350
EREAATDELH HDTLAELTGE GKPFEGRADE VNGAYQALTK QTVRQRILKD
360 370 380 390 400
KVRIDGRGLT DIRQLSAEVD VLPRVHGSAL FERGETQIMG VTTLNMLKME
410 420 430 440 450
QQIDSLSPVK HKRYIHHYNF PPYSTGETGR VGSPKRREIG HGALAERAIT
460 470 480 490 500
PVLPSREEFP YAIRQVSEAL GSNGSTSMGS VCASTLALYN AGVPLRAPVA
510 520 530 540 550
GIAMGLVSDT VDGEVQYAAL TDILGAEDAL GDMDFKVAGT REFVTAIQLD
560 570 580 590 600
TKLDGIPASV LASALTQARE ARLYILDVLT SAIDAPDEMS EFAPRVISVT
610 620 630 640 650
VPVSKIGEVI GPKGKMINQI QEDTGTDISI EDDGTVYIGA TDGPSAEAAR
660 670 680 690 700
SAINAIANPQ VPEVGERYLG TVVKTTTFGA FVSLTPGKDG LLHISEMRQL
710 720 730 740 750
NDDKRVNEVD DVLSVGQKVQ VEITKVDDRG KLSLAPVVAG SDSDAASDDE

N
Length:751
Mass (Da):80,534
Last modified:June 10, 2008 - v1
Checksum:iEDEAE9978EDE5A90
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009152 Genomic DNA. Translation: BAG29942.1.
RefSeqiWP_012398663.1. NC_010617.1.

Genome annotation databases

EnsemblBacteriaiBAG29942; BAG29942; KRH_15950.
KEGGikrh:KRH_15950.
PATRICi22183488. VBIKocRhi5258_1591.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009152 Genomic DNA. Translation: BAG29942.1.
RefSeqiWP_012398663.1. NC_010617.1.

3D structure databases

ProteinModelPortaliB2GKI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi378753.KRH_15950.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG29942; BAG29942; KRH_15950.
KEGGikrh:KRH_15950.
PATRICi22183488. VBIKocRhi5258_1591.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciKRHI378753:GJ8F-1635-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR014069. pppGpp_PNP.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
TIGR02696. pppGpp_PNP. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Entry informationi

Entry nameiPNP_KOCRD
AccessioniPrimary (citable) accession number: B2GKI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: July 22, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.