B2GJQ6 (RSMH_KOCRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	KRH_14900

Organism

Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) [Complete proteome] [HAMAP]

Taxonomic identifier

378753 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Kocuria

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000386937
Regions
	Region	41 – 43	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	60	1	S-adenosyl-L-methionine By similarity
	Binding site	87	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	108	1	S-adenosyl-L-methionine By similarity
	Binding site	115	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2GJQ6 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 0B1C2D203A5A3277	FASTA	327	36,125
	10 20 30 40 50 60 MEEAPAEQRH VPVLLDRCLD LLAPALDTEA PVVLDATLGM GGHTHAMLER FPHLRVVGID 70 80 90 100 110 120 RDPQALELAG RRLQRFGSRF ASIHTTYDHI ADAAAAAGVE RVDGVLFDLG VSSLQLDERE 130 140 150 160 170 180 RGFAYSFDAP LDMRMDSRAE LSAYTVVNEY SEARLRDIIF RWGEDRFAPR IARAIVAARA 190 200 210 220 230 240 DAPLRTTAQL VDAVRSAVPA AAQHKKGHPA KQTFQALRIE VNEELDVLER AIPAAVETVR 250 260 270 280 290 300 VGGRVIAMSY HSLEDRIVKQ EFARGARSTA PRGFPVELPE HQPVLKVLTR GAERADEQET 310 320 LENPRAASAR LRAVERLRET TTPGSAR

References

[1]

"Complete genome sequence of the soil actinomycete Kocuria rhizophila." Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S., Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. J. Bacteriol. 190:4139-4146(2008) [PubMed: 18408034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009152 Genomic DNA. Translation: BAG29837.1.
RefSeq	YP_001855343.1. NC_010617.1.
3D structure databases
ProteinModelPortal	B2GJQ6.
ModBase	Search...
Protein-protein interaction databases
STRING	B2GJQ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6238573.
GenomeReviews	Gene locus KRH_14900 in contig AP009152_GR.
KEGG	krh:KRH_14900.
PATRIC	22183262. VBIKocRhi5258_1482.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_KOCRD

Accession

Primary (citable) accession number: B2GJQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program