B2GH68 (B2GH68_KOCRD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL BAG28820.1
Ordered Locus Names:	KRH_04730

Organism

Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) [Complete proteome] [HAMAP]

Taxonomic identifier

378753 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Kocuria

Protein attributes

Sequence length	980 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B2GH68 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 5ECAA7253260C05C	FASTA	980	106,661
	10 20 30 40 50 60 MASKATKNEG TGKSLLIVES PSKVKTIAGY LGDAYEVESS MGHIRDLPQP SELPTDMKKG 70 80 90 100 110 120 PYGKFAVDVE HEFDPYYVVN PDKKKKVTEL KRKLKDADAL YLATDGDREG EAIAWHLLQV 130 140 150 160 170 180 LKPKVPVYRL TFPEITKEAI ERGFGQLRDI DQDLVDAQET RRILDRLYGY EISPVLWRKV 190 200 210 220 230 240 AAGLSAGRVQ SVATRLVVQR ERERIAFVPA DYWDLTGQFS RPEGQDAGRA FSARLSSVNG 250 260 270 280 290 300 RRVATGKDFD DRGQLKSPSS GNAPVTLDEA VATALAAGLQ DAPFSVRSLE TKPYTRRPAA 310 320 330 340 350 360 PFTTSTLQQE AARKLRFTSR TTMRVAQRLY ENGYITYMRT DSVALSDQAV NAARAQAKEL 370 380 390 400 410 420 YGPEFVPSSK RVYASKSKNA QEAHEAVRPA GDTFRTPSQV RSQLSVDEYK LYELIWKRTV 430 440 450 460 470 480 ASQMQDAKGD TATVRLGATA TSAAGEHAGA DAEFSASGTV ITFRGFLAAY EEGRDVNPDL 490 500 510 520 530 540 TADSGKKGAK DEDKRLPVMA EGDALDASEV TADGHTTTPP ARYTEASLVK TLDELGIGRP 550 560 570 580 590 600 STYAATISTI MDRGYVQVRG SALIPSWLAF SVVRLLEDHF SDYVDYDFTA ELEDDLDRIA 610 620 630 640 650 660 RGEAERVKWL SGFYFGENPS EPGLKPIVDD LGEIDARAVN SIPITDEITL RVGKYGPYLE 670 680 690 700 710 720 AGGTVDPETG EITDPVRANV PLDLAPDELT PEKAQELMEI GKADGRELGV NPANGRTIVA 730 740 750 760 770 780 KDGRYGPYVT EIVPEPTQEE LDAIPTEYYK NGNPKPKKIE KPKPNTASLF SSMNLQDITL 790 800 810 820 830 840 DDALKLLSLP RELGQDTDGE TITVQNGRYG PYLKKGSDSR SISSEDQIFT ITLEEAKTIY 850 860 870 880 890 900 AQPKQRGRQA ARPPLADLGA DPLTEKRMVI KEGRFGAYIT DGETNVTVPR AEAVEQITAA 910 920 930 940 950 960 RASQLLAEKR AKGPAPAKSP ARGKARTASK SSTSKASASK GTSSKAKAGT TTAKRASATK 970 980 AKSTTGAART TRKAPAKKSS

References

[1]

"Complete genome sequence of the soil actinomycete Kocuria rhizophila." Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S., Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. J. Bacteriol. 190:4139-4146(2008) [PubMed: 18408034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009152 Genomic DNA. Translation: BAG28820.1.
RefSeq	YP_001854326.1. NC_010617.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2GH68.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6237943.
GenomeReviews	Gene locus KRH_04730 in contig AP009152_GR.
KEGG	krh:KRH_04730.
PATRIC	22181174. VBIKocRhi5258_0462.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	CLSK960232.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B2GH68_KOCRD

Accession

Primary (citable) accession number: B2GH68

Entry history

Integrated into UniProtKB/TrEMBL:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)