B2GFZ7 (FABH_KOCRD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.180

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	KRH_10780

Organism

Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) [Complete proteome] [HAMAP]

Taxonomic identifier

378753 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Kocuria

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm By similarity HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000187871
Regions
	Region	281 – 285	5	ACP-binding By similarity
Sites
	Active site	122	1	By similarity
	Active site	280	1	By similarity
	Active site	311	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2GFZ7 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 44D63DC036DCA093	FASTA	355	37,822
	10 20 30 40 50 60 MATIKQNIPH AHTRILGIGA YRPSEIVTND DVCQWIESSD EWIVKRTGIH TRHRAPEDVS 70 80 90 100 110 120 VLDMAEHAAR EALENSGLTG EQLSTIIVST VSFPYLTPSL AANLADRLGA TPAAAYDISA 130 140 150 160 170 180 ACAGYCYGVA QADSLVRSGD STNVLVIGVE KLSDVIDNHE RSISFLLGDG AGAVVIGPSE 190 200 210 220 230 240 VPGIGPSVWG SDGSKWDAIR MTHPLTDIRT VERGGQRTFA KDLVDPETGE MDPDATLWPT 250 260 270 280 290 300 LRQDGQTVFR WASWEGARVA QEALDAAGLA PEDLAAFVPH QANMRIIDQM AKVLKLPESV 310 320 330 340 350 IIARDIAEAG NTSAASIPLA THRLLAEHPE LSGKPSLQIG FGAGLVYGAQ VVLLP

References

[1]

"Complete genome sequence of the soil actinomycete Kocuria rhizophila." Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S., Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S. J. Bacteriol. 190:4139-4146(2008) [PubMed: 18408034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009152 Genomic DNA. Translation: BAG29425.1.
RefSeq	YP_001854931.1. NC_010617.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2GFZ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6239250.
GenomeReviews	Gene locus KRH_10780 in contig AP009152_GR.
KEGG	krh:KRH_10780.
PATRIC	22182390. VBIKocRhi5258_1058.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649927.
OMA	SNCFIFA.
ProtClustDB	PRK09352.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 3 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_KOCRD

Accession

Primary (citable) accession number: B2GFZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program