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B2GFY4 (TRMD_KOCRD) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:KRH_10650
OrganismKocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) [Complete proteome] [HAMAP]
Taxonomic identifier378753 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Kocuria

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605

Subunit structure

Homodimer By similarity. HAMAP MF_00605

Subcellular location

Cytoplasm Potential HAMAP MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (guanine-N1-)-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
PRO_1000130181

Regions

Region193 – 1986S-adenosyl-L-methionine binding By similarity

Sites

Binding site1691S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B2GFY4 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 6AA66BD23600AEF9

FASTA33135,752
        10         20         30         40         50         60 
MRIDVLSIFP EYLEPLKLSL IGKAVTDGLL QLQVTDPREF ATDRHRTVDD TPYGGGAGMV 

        70         80         90        100        110        120 
MKPEPWARAL ESVLRAGSDT TARSGSTAAT SASAQQATRL GPDDDAAQPG DAGQGTAAPD 

       130        140        150        160        170        180 
YARTGGTPGA GRAASSRPVL VVPSPAGEVF TQEVAYELAE EEHLVFACGR YEGIDERFIE 

       190        200        210        220        230        240 
WARDEVRVRP LSLGDYVLNG GEVAVLAMVE AVTRLVPGVI GNPESLDEES HTGGLLEYPV 

       250        260        270        280        290        300 
YTKPAQWRER TVPDTLLSGH HGRIARWRRD QQLERTARRR PDMVLALDPA TLDRADLAVL 

       310        320        330 
AAEGFTLRDG QWQRCSPAPS EQAPEGARDM A

« Hide

References

[1]"Complete genome sequence of the soil actinomycete Kocuria rhizophila."
Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S., Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
J. Bacteriol. 190:4139-4146(2008) [PubMed: 18408034] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP009152 Genomic DNA. Translation: BAG29412.1.
RefSeqYP_001854918.1. NC_010617.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB2GFY4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6239801.
GenomeReviewsGene locus KRH_10650 in contig AP009152_GR.
KEGGkrh:KRH_10650.
PATRIC22182348. VBIKocRhi5258_1037.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG285805.
OMANRYDLYL.

Family and domain databases

HAMAPMF_00605. TrmD.
[Tree]
InterProIPR016009. tRNA_m1G_MeTrfase.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
[Graphical view]
Gene3DG3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KOK00554.
PANTHERPTHR10056. PTHR10056. 1 hit.
PfamPF01746. tRNA_m1G_MT. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMD_KOCRD
AccessionPrimary (citable) accession number: B2GFY4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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