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B2GFL1 (DDL_KOCRD) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:KRH_10370
OrganismKocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) [Complete proteome] [HAMAP]
Taxonomic identifier378753 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeKocuria

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 383383D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091188

Regions

Domain164 – 373210ATP-grasp
Nucleotide binding196 – 25156ATP By similarity

Sites

Metal binding3271Magnesium or manganese 1 By similarity
Metal binding3401Magnesium or manganese 1 By similarity
Metal binding3401Magnesium or manganese 2 By similarity
Metal binding3421Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B2GFL1 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 06B548C65AB11521

FASTA38341,365
        10         20         30         40         50         60 
MSTTHETPEA TAEGDKPCIA VVFGGRSSEH SISLITARSV LRAIDRDRWD VVSVGISTDG 

        70         80         90        100        110        120 
AWFLCSQEEL EALLDDQPMA QLPVGVHRVS LPLQTGDSRL LIHDTSEHGA PLSRGRHIDA 

       130        140        150        160        170        180 
VFPLLHGPFG EDGTLQGMLE LADLPYVGCG VAASAIGMDK HFMKLAFQAA GLEVGPYTVV 

       190        200        210        220        230        240 
HDRTWRTDPL GVREAVAELG FPVFVKPARA GSSFGITRVD EPSQLDAAIA TAREHDLKLV 

       250        260        270        280        290        300 
VEAGIDGREI ECAVLGGHGT DEARASLPGE IEVHGHALYD FEAKYVESDG ATLSCPARLP 

       310        320        330        340        350        360 
EHVIDTLRRD AVRAFHAVDG EGLSRCDFFV TADERVLINE INTMPGFTPI SMYPRMWAAS 

       370        380 
GIDYSALIDE LITLALERPV GLR 

« Hide

References

[1]"Complete genome sequence of the soil actinomycete Kocuria rhizophila."
Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S., Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
J. Bacteriol. 190:4139-4146(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009152 Genomic DNA. Translation: BAG29384.1.
RefSeqYP_001854890.1. NC_010617.1.

3D structure databases

ProteinModelPortalB2GFL1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING378753.KRH_10370.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG29384; BAG29384; KRH_10370.
GeneID6239472.
KEGGkrh:KRH_10370.
PATRIC22182290. VBIKocRhi5258_1008.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAIECGVLG.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycKRHI378753:GJ8F-1060-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_KOCRD
AccessionPrimary (citable) accession number: B2GFL1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways