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B2GFK6 (B2GFK6_KOCRD) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydratase large subunit HAMAP-Rule MF_01026
EC=4.2.1.33 HAMAP-Rule MF_01026
Alternative name(s):
Alpha-IPM isomerase HAMAP-Rule MF_01026
Isopropylmalate isomerase HAMAP-Rule MF_01026
Gene names
Name:leuC HAMAP-Rule MF_01026 EMBL BAG29379.1
Ordered Locus Names:KRH_10320
OrganismKocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201) [Complete proteome] [HAMAP]
Taxonomic identifier378753 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Kocuria ›

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01026 SAAS SAAS004430

Catalytic activity

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01026

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01026 SAAS SAAS015931

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01026 SAAS SAAS004430

Subunit structure

Heterodimer of LeuC and LeuD By similarity. HAMAP-Rule MF_01026 SAAS SAAS004430

Sequence similarities

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily. HAMAP-Rule MF_01026

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding3561Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01026
Metal binding4161Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01026
Metal binding4191Iron-sulfur (4Fe-4S) By similarity HAMAP-Rule MF_01026

Sequences

Sequence LengthMass (Da)Tools
B2GFK6 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 0C873AF332550070

FASTA47851,378
        10         20         30         40         50         60 
MSTTTQTAAP RTLAQKVWDD HLVRRGEGVH PDLLYIDLHL LHEVTSPQAF EGLRLAGRGL 

        70         80         90        100        110        120 
RRTDLTIATE DHNTPTEDIF STIADETSRK QIETLRRNCE EFGVRLHSLG DAEQGIVHVV 

       130        140        150        160        170        180 
GPQLGLTQPG MTVVCGDSHT STHGAFGALA FGIGTSEVEH VMATQTLPLA PFKTMAITVE 

       190        200        210        220        230        240 
GTLKPGVSSK DIILAVIAKI GTGGGQGYVL EYRGSAIRSL SMEARMTMCN MSIEAGARAG 

       250        260        270        280        290        300 
MIAPDQTTFD YVQGRPHAPQ GEEWDVAVEY WRGLRTDEGA QFDREVVIDA DKLEPFVTWG 

       310        320        330        340        350        360 
TNPGQGVPLS RAVPDPEDAG DDDAKDAARR ALSYMGLQAG TPMKQIPVDT VFLGSCTNSR 

       370        380        390        400        410        420 
IEDLRAAAEI LKGRSKAETV RMMVVPGSAK VRLQAEAEGL DRVFREFGAD WRFAGCSMCL 

       430        440        450        460        470 
GMNPDQLAPG ERCASTSNRN FEGRQGKGGR THLVSPVVAA ATAVRGTLSA PSDLDPVA

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References

[1]"Complete genome sequence of the soil actinomycete Kocuria rhizophila."
Takarada H., Sekine M., Kosugi H., Matsuo Y., Fujisawa T., Omata S., Kishi E., Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.
J. Bacteriol. 190:4139-4146(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 9341 / DSM 348 / NBRC 103217 / DC2201.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP009152 Genomic DNA. Translation: BAG29379.1.
RefSeqYP_001854885.1. NC_010617.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING378753.KRH_10320.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG29379; BAG29379; KRH_10320.
GeneID6238309.
KEGGkrh:KRH_10320.
PATRIC22182280. VBIKocRhi5258_1003.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0065.
HOGENOMHOG000226972.
KOK01703.
OMADIRQGIV.
ProtClustDBCLSK2801438.

Enzyme and pathway databases

BioCycKRHI378753:GJ8F-1055-MONOMER.
UniPathwayUPA00048; UER00071.

Family and domain databases

Gene3D3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPMF_01026. LeuC_type1.
InterProIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PfamPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF53732. Aconitase_N. 1 hit.
TIGRFAMsTIGR00170. leuC. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB2GFK6_KOCRD
AccessionPrimary (citable) accession number: B2GFK6
Entry history
Integrated into UniProtKB/TrEMBL: June 10, 2008
Last sequence update: June 10, 2008
Last modified: May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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