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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei271ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciKRHI378753:G1G2M-1022-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:KRH_10280
OrganismiKocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201)
Taxonomic identifieri378753 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeKocuria
Proteomesi
  • UP000008838 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003676941 – 506Glutamate--tRNA ligaseAdd BLAST506

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi378753.KRH_10280

Structurei

3D structure databases

SMRiB2GFK2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi24 – 34"HIGH" regionUniRule annotationAdd BLAST11
Motifi268 – 272"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252720
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
1.10.1160.10, 1 hit
3.40.50.620, 2 hits
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020061 Glu_tRNA_lig_a-bdl
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit

Sequencei

Sequence statusi: Complete.

B2GFK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDDASTLHP TVPADAPVRV RFCPSPTGTP HVGLIRTALF NWAWARHTGG
60 70 80 90 100
TLVFRIEDTD AARDSEESFQ QILDALDWLG ITWDEGVNVG GPHEPYRQSQ
110 120 130 140 150
RGEIYQDVLA KLVDSGYVYE DFSTPEEVEQ RHRAKGEDPK RGYDNYDRDL
160 170 180 190 200
TDEQKAAFRA EGRQPVLRLR MPDEDISFTD LVRGEITFKA GSVPDFVVAR
210 220 230 240 250
ANGKPLYTLV NPVDDALMGI TQVLRGEDLL SSTPRQIALY RALVAIGVAE
260 270 280 290 300
FIPEFGHLPY VMGEGNKKLS KRDPESNLFH HRDNGFIPEG LLNYLALLGW
310 320 330 340 350
SLSADEDIFT VEQLVANFDI HDVLANPARF DVKKATAING THVRMLDPQD
360 370 380 390 400
FRDRLVPYLR AAGLVGETLT PRENEILDRA APLVQERMNL LGETEGLLGF
410 420 430 440 450
LFRGDDEIVV APDAAKQLKD SAPEVLDAAL AALEPLDDFT AEAIETALRE
460 470 480 490 500
AIVDGLGIKP RLAFGPVRTA VSGQRISPPL FESLEILGRE SSLARIAALR

AELAAN
Length:506
Mass (Da):55,925
Last modified:June 10, 2008 - v1
Checksum:iC10CE1BD0C9FC98E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009152 Genomic DNA Translation: BAG29375.1
RefSeqiWP_012398096.1, NC_010617.1

Genome annotation databases

EnsemblBacteriaiBAG29375; BAG29375; KRH_10280
GeneIDi35827874
KEGGikrh:KRH_10280

Similar proteinsi

Entry informationi

Entry nameiSYE_KOCRD
AccessioniPrimary (citable) accession number: B2GFK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: April 25, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health