B2GFD7 (ISPE_LACF3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	LAF_0190

Organism

Lactobacillus fermentum (strain NBRC 3956 / LMG 18251) [Complete proteome] [HAMAP]

Taxonomic identifier

334390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092092
Regions
	Nucleotide binding	95 – 105	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	137	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2GFD7 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: D7A090D83C25EEE3	FASTA	283	31,482
	10 20 30 40 50 60 MIVTEKAPAK INLALDTPMR YLDGLPRWNM VMNAVDLADY VTVEIHHRPQ TIKVYTNSGF 70 80 90 100 110 120 LPNDQRNLAY QAAHILKTRF HQTDGVTIRI KKKIPVAAGL GGGSSDAAAV LRALNKAWRL 130 140 150 160 170 180 GLSLDELARL SLSIDSDVPF CVYSQTAHVT GHGEIVEPLP AFPHYWVVIA KPKLSVSTPV 190 200 210 220 230 240 ILRQINYERL VHPQTDQLVE AIKEGKFQES FQYMGNALEA VTMEAHPEIA RLKERMQKLG 250 260 270 280 ADVAQMSGTG PSVFALCHAE SRAKRIYNSL RGFCPEVYQV VLL

References

[1]

"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production." Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M. DNA Res. 15:151-161(2008) [PubMed: 18487258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NBRC 3956 / LMG 18251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008937 Genomic DNA. Translation: BAG26526.1.
RefSeq	YP_001843006.1. NC_010610.1.
3D structure databases
ProteinModelPortal	B2GFD7.
ModBase	Search...
Protein-protein interaction databases
STRING	B2GFD7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6233463.
GenomeReviews	Gene locus LAF_0190 in contig AP008937_GR.
KEGG	lfe:LAF_0190.
PATRIC	22224372. VBILacFer15497_0209.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	VSTAEVY.
ProtClustDB	PRK00128.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_LACF3

Accession

Primary (citable) accession number: B2GFD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program