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Protein

Phosphoribosylformylglycinamidine cyclo-ligase

Gene

purM

Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathway: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS), Phosphoribosylformylglycinamidine synthase subunit PurL (purL)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLFER334390:GJ2S-131-MONOMER.
UniPathwayiUPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligaseUniRule annotation (EC:6.3.3.1UniRule annotation)
Alternative name(s):
AIR synthaseUniRule annotation
AIRSUniRule annotation
Phosphoribosyl-aminoimidazole synthetaseUniRule annotation
Gene namesi
Name:purMUniRule annotation
Ordered Locus Names:LAF_0129
OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Taxonomic identifieri334390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001697 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Phosphoribosylformylglycinamidine cyclo-ligasePRO_1000193029Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0150.
HOGENOMiHOG000229090.
KOiK01933.
OMAiNHCVNDI.
OrthoDBiEOG61CM1V.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.

Sequencei

Sequence statusi: Complete.

B2GF76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRYQEAGVD VNAGYELVKR IKENVKSTNR PGALGGIGAF GGLFDLGELK
60 70 80 90 100
VKHPVLVSGT DGVGTKLLIA KAMDQHDTIG IDVVAMCVND VLAQGAEPLY
110 120 130 140 150
FLDYIATGHN DPAKMADIVA GVAEGCRQAG AALIGGETAE MPDMYDQDEY
160 170 180 190 200
DLAGTVTGVV EKDDLLTSDQ PQAGAILLGL ASSGIHSNGF SLVRQILFKD
210 220 230 240 250
HQVDLAATPA KLGGQTVGQA VLAPTKIYVK PVLPLLKDHL VEGVAHITGG
260 270 280 290 300
GLIENLPRMF GDDLQAQVDS SAWPSLPVFD YLKELGGLSD DDCWQTFNMG
310 320 330 340
LGMILAVQPD LVDQVKSRLN QAGEACYQVG QLVNRPAGEE KIVIK
Length:345
Mass (Da):36,521
Last modified:June 10, 2008 - v1
Checksum:i4D9F61D76F5E6AEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008937 Genomic DNA. Translation: BAG26465.1.
RefSeqiWP_012390753.1. NC_010610.1.
YP_001842945.1. NC_010610.1.

Genome annotation databases

EnsemblBacteriaiBAG26465; BAG26465; LAF_0129.
GeneIDi6232602.
KEGGilfe:LAF_0129.
PATRICi22224208. VBILacFer15497_0135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008937 Genomic DNA. Translation: BAG26465.1.
RefSeqiWP_012390753.1. NC_010610.1.
YP_001842945.1. NC_010610.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG26465; BAG26465; LAF_0129.
GeneIDi6232602.
KEGGilfe:LAF_0129.
PATRICi22224208. VBILacFer15497_0135.

Phylogenomic databases

eggNOGiCOG0150.
HOGENOMiHOG000229090.
KOiK01933.
OMAiNHCVNDI.
OrthoDBiEOG61CM1V.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00129.
BioCyciLFER334390:GJ2S-131-MONOMER.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00741. AIRS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00878. purM. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 3956 / LMG 18251.

Entry informationi

Entry nameiPUR5_LACF3
AccessioniPrimary (citable) accession number: B2GF76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: June 24, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.