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Protein

Formate--tetrahydrofolate ligase

Gene

fhs

Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 708ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLFER334390:GJ2S-119-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligaseUniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetaseUniRule annotation
Short name:
FHSUniRule annotation
Short name:
FTHFSUniRule annotation
Gene namesi
Name:fhsUniRule annotation
Ordered Locus Names:LAF_0117
OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Taxonomic identifieri334390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001697 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Formate--tetrahydrofolate ligasePRO_1000196811Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi334390.LAF_0117.

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2GF64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTDIEIADQ AQLTPINEIA AQLGLDEDAI EQYGKYKAKI NLPVQATPEK
60 70 80 90 100
KHKLVLVTSI NPTPAGEGKS TVLVGLGDAL SLLHHQTVIA MREPSMGPVF
110 120 130 140 150
GMKGGATGGG YSQVVPMEDI NLHFTGDFHA LTSANNTLAA LIDNYLMRGN
160 170 180 190 200
ELGLDPRRVI WKRVEDVNDR ALRDVVTGLG GIMQGVPRQT GFDITPASEL
210 220 230 240 250
MAILCLATDL SDLKARVSRI VVGYTYDKEP VTVGQLGFEE AVTILLKDAI
260 270 280 290 300
KPNLVQTLGH TPAIVHGGPF ANIAHGCNSV LATKTALQLA DYTVTEAGFG
310 320 330 340 350
ADLGAEKFLD IKRPVLGKTP DAVVIVATVR ALEYNGGASL QALKDENLTE
360 370 380 390 400
LENGLQNLNR HIANMQRYGL PLVVAINHFA TDTPAEIKLI EDNCKARGVN
410 420 430 440 450
VVVADAWAKG GAGTLDLAKE VVALAEQEAS FTPLYDYQAT PKEKVETIAT
460 470 480 490 500
KVYGAGRVAF SKKALNQLKQ FEKLGWNDLP ICIAKTQYSF TDDQTQLGAP
510 520 530 540 550
EGFTFHIREF VPKLGAGFIV ALAGNMLTMP GLPKVPAAVK MTIDSEGKIT

GLF
Length:553
Mass (Da):59,287
Last modified:June 9, 2008 - v1
Checksum:i8BED7428F0A9E7AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008937 Genomic DNA. Translation: BAG26453.1.
RefSeqiWP_012390743.1. NC_010610.1.
YP_001842933.1. NC_010610.1.

Genome annotation databases

EnsemblBacteriaiBAG26453; BAG26453; LAF_0117.
GeneIDi6232948.
KEGGilfe:LAF_0117.
PATRICi22224182. VBILacFer15497_0122.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008937 Genomic DNA. Translation: BAG26453.1.
RefSeqiWP_012390743.1. NC_010610.1.
YP_001842933.1. NC_010610.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi334390.LAF_0117.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG26453; BAG26453; LAF_0117.
GeneIDi6232948.
KEGGilfe:LAF_0117.
PATRICi22224182. VBILacFer15497_0122.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiCMVKTHL.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciLFER334390:GJ2S-119-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 3956 / LMG 18251.

Entry informationi

Entry nameiFTHS_LACF3
AccessioniPrimary (citable) accession number: B2GF64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2009
Last sequence update: June 9, 2008
Last modified: March 3, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.