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B2GF64 (FTHS_LACF3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:LAF_0117
OrganismLactobacillus fermentum (strain NBRC 3956 / LMG 18251) [Complete proteome] [HAMAP]
Taxonomic identifier334390 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 553553Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_1000196811

Regions

Nucleotide binding63 – 708ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2GF64 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 8BED7428F0A9E7AA

FASTA55359,287
        10         20         30         40         50         60 
MLTDIEIADQ AQLTPINEIA AQLGLDEDAI EQYGKYKAKI NLPVQATPEK KHKLVLVTSI 

        70         80         90        100        110        120 
NPTPAGEGKS TVLVGLGDAL SLLHHQTVIA MREPSMGPVF GMKGGATGGG YSQVVPMEDI 

       130        140        150        160        170        180 
NLHFTGDFHA LTSANNTLAA LIDNYLMRGN ELGLDPRRVI WKRVEDVNDR ALRDVVTGLG 

       190        200        210        220        230        240 
GIMQGVPRQT GFDITPASEL MAILCLATDL SDLKARVSRI VVGYTYDKEP VTVGQLGFEE 

       250        260        270        280        290        300 
AVTILLKDAI KPNLVQTLGH TPAIVHGGPF ANIAHGCNSV LATKTALQLA DYTVTEAGFG 

       310        320        330        340        350        360 
ADLGAEKFLD IKRPVLGKTP DAVVIVATVR ALEYNGGASL QALKDENLTE LENGLQNLNR 

       370        380        390        400        410        420 
HIANMQRYGL PLVVAINHFA TDTPAEIKLI EDNCKARGVN VVVADAWAKG GAGTLDLAKE 

       430        440        450        460        470        480 
VVALAEQEAS FTPLYDYQAT PKEKVETIAT KVYGAGRVAF SKKALNQLKQ FEKLGWNDLP 

       490        500        510        520        530        540 
ICIAKTQYSF TDDQTQLGAP EGFTFHIREF VPKLGAGFIV ALAGNMLTMP GLPKVPAAVK 

       550 
MTIDSEGKIT GLF 

« Hide

References

[1]"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBRC 3956 / LMG 18251.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008937 Genomic DNA. Translation: BAG26453.1.
RefSeqYP_001842933.1. NC_010610.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING334390.LAF_0117.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG26453; BAG26453; LAF_0117.
GeneID6232948.
KEGGlfe:LAF_0117.
PATRIC22224182. VBILacFer15497_0122.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMACGEIMTM.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13505.

Enzyme and pathway databases

BioCycLFER334390:GJ2S-119-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_LACF3
AccessionPrimary (citable) accession number: B2GF64
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: April 16, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways