B2GE56 (ASNA_LACF3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	LAF_1602

Organism

Lactobacillus fermentum (strain NBRC 3956 / LMG 18251) [Complete proteome] [HAMAP]

Taxonomic identifier

334390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000129121

Sequences

	Sequence		Length	Mass (Da)
	B2GE56 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: FD08E21DEE4BDBB4	FASTA	336	38,795
	10 20 30 40 50 60 MSLIVPENYD PKLSIRETEE AIRYIRETFQ DEIGKELNLQ RMSAPMFVEQ STGLNDNLNG 70 80 90 100 110 120 VEAPVSFTMK DLPGETIEIV HSLAKWKRFA LKKYGFGLHE GLYTNMNAIR KDEDLDNFHS 130 140 150 160 170 180 IYVDQWDWEK VIAKEERTEA TLKATVRQVF KVIKHMEHEV WYKFPQAVYH LPDEIHFVTT 190 200 210 220 230 240 QGLEDRWPEL SPMEREDKIA KELGCVFVMK IGDKLQRSGE PHDGRAPDYD DWSLNGDIIF 250 260 270 280 290 300 WYEPLQTKLE VSSMGIRVDE RAMKEQLEKA HATDRAELPF HRDLLAGKMP YTIGGGIGQS 310 320 330 RLCMLLLGKA HVGEVQASIW PEEMKEACAK AKIQLL

References

[1]

"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production." Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M. DNA Res. 15:151-161(2008) [PubMed: 18487258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NBRC 3956 / LMG 18251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008937 Genomic DNA. Translation: BAG27938.1.
RefSeq	YP_001844418.1. NC_010610.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2GE56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6233252.
GenomeReviews	Gene locus LAF_1602 in contig AP008937_GR.
KEGG	lfe:LAF_1602.
PATRIC	22227604. VBILacFer15497_1763.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_LACF3

Accession

Primary (citable) accession number: B2GE56

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program