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B2GDF8 (SYL_LACF3) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:LAF_1354
OrganismLactobacillus fermentum (strain NBRC 3956 / LMG 18251) [Complete proteome] [HAMAP]
Taxonomic identifier334390 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length805 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 805805Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091326

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif577 – 5815"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5801ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2GDF8 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: FF59FADFE65E77FA

FASTA80592,621
        10         20         30         40         50         60 
MAYDHKAIEK KWQRYWKQHK TFKATLDKDQ KKYYALDMFP YPSGQGLHVG HPEGYTATDV 

        70         80         90        100        110        120 
MSRLKRMQGF NVLHPMGWDA FGLPAEQYAL KTGHNPADFT NQNVDHFRDQ IQSLGFSYDW 

       130        140        150        160        170        180 
DREVNTTDPN YYKWTQWIFE QLYKKGLAYE DEIMVNWAPD FMGGTVVANE EVVDGKTERG 

       190        200        210        220        230        240 
GYPVYRVPMR QWVLKITAYA DRLIDDLDLV DWPESVKEMQ RNWIGRSEGA SVKFKVVGHD 

       250        260        270        280        290        300 
GVEIEVFTTR ADTLFGASYV VLAPENELVD QLTTPEQKAA VDAYKEEVSR RSDLERTELS 

       310        320        330        340        350        360 
KEKTGVFTGA YVINPVNGEQ LPIWTADYVL NSYGTGAVMA VPSGDQRDFE FATKFNLPIT 

       370        380        390        400        410        420 
PVVEGFNGEE AYTEDGAHVN SGFLDGLNIK EAKAKMVEWL EEHDCGGKKV NYRLRDWIFS 

       430        440        450        460        470        480 
RQRYWGEPIP VIHWDDGTTS LVPEDELPLR LPETDNIEPS GTGESPLANI EDWVNVYDEN 

       490        500        510        520        530        540 
GRHGKRETNT MPQWAGSSWY WLRYTDPTND KEFASKEALD YWSPVDLYVG GAEHAVLHLL 

       550        560        570        580        590        600 
YARFWHKVLY DLGLVPTKEP FMKLVNQGMI LGSNHEKMSK SKGNVVNPDD IVDQYGADTL 

       610        620        630        640        650        660 
RLYEMFMGPL EESVPWDEKG LHGSNKWVQR VWRLLMDDNN HLRDRVSTYN DGKLTKVYNQ 

       670        680        690        700        710        720 
TVKKVTDDFE RMHFNTAISQ LMVFVNEAYK VDDLPLEYMK GFVKMIAPLM PHLAEELWSQ 

       730        740        750        760        770        780 
FNESETITYQ PWPTYDEKAL VEDEVEMIVQ VNGKVRAKIK MAKDADNKDV EDAALANEHV 

       790        800 
HSFVDGKDVK KVIVIPNRIV NIVVK 

« Hide

References

[1]"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBRC 3956 / LMG 18251.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008937 Genomic DNA. Translation: BAG27690.1.
RefSeqYP_001844170.1. NC_010610.1.

3D structure databases

ProteinModelPortalB2GDF8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING334390.LAF_1354.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG27690; BAG27690; LAF_1354.
GeneID6232679.
KEGGlfe:LAF_1354.
PATRIC22226994. VBILacFer15497_1479.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycLFER334390:GJ2S-1403-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACF3
AccessionPrimary (citable) accession number: B2GDF8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: June 11, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries