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B2GDF8

- SYL_LACF3

UniProt

B2GDF8 - SYL_LACF3

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei580 – 5801ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciLFER334390:GJ2S-1403-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:LAF_1354
    OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
    Taxonomic identifieri334390 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000001697: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 805805Leucine--tRNA ligasePRO_1000091326Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi334390.LAF_1354.

    Structurei

    3D structure databases

    ProteinModelPortaliB2GDF8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi40 – 5112"HIGH" regionAdd
    BLAST
    Motifi577 – 5815"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200748.
    KOiK01869.
    OMAiDKPKYYA.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2GDF8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYDHKAIEK KWQRYWKQHK TFKATLDKDQ KKYYALDMFP YPSGQGLHVG    50
    HPEGYTATDV MSRLKRMQGF NVLHPMGWDA FGLPAEQYAL KTGHNPADFT 100
    NQNVDHFRDQ IQSLGFSYDW DREVNTTDPN YYKWTQWIFE QLYKKGLAYE 150
    DEIMVNWAPD FMGGTVVANE EVVDGKTERG GYPVYRVPMR QWVLKITAYA 200
    DRLIDDLDLV DWPESVKEMQ RNWIGRSEGA SVKFKVVGHD GVEIEVFTTR 250
    ADTLFGASYV VLAPENELVD QLTTPEQKAA VDAYKEEVSR RSDLERTELS 300
    KEKTGVFTGA YVINPVNGEQ LPIWTADYVL NSYGTGAVMA VPSGDQRDFE 350
    FATKFNLPIT PVVEGFNGEE AYTEDGAHVN SGFLDGLNIK EAKAKMVEWL 400
    EEHDCGGKKV NYRLRDWIFS RQRYWGEPIP VIHWDDGTTS LVPEDELPLR 450
    LPETDNIEPS GTGESPLANI EDWVNVYDEN GRHGKRETNT MPQWAGSSWY 500
    WLRYTDPTND KEFASKEALD YWSPVDLYVG GAEHAVLHLL YARFWHKVLY 550
    DLGLVPTKEP FMKLVNQGMI LGSNHEKMSK SKGNVVNPDD IVDQYGADTL 600
    RLYEMFMGPL EESVPWDEKG LHGSNKWVQR VWRLLMDDNN HLRDRVSTYN 650
    DGKLTKVYNQ TVKKVTDDFE RMHFNTAISQ LMVFVNEAYK VDDLPLEYMK 700
    GFVKMIAPLM PHLAEELWSQ FNESETITYQ PWPTYDEKAL VEDEVEMIVQ 750
    VNGKVRAKIK MAKDADNKDV EDAALANEHV HSFVDGKDVK KVIVIPNRIV 800
    NIVVK 805
    Length:805
    Mass (Da):92,621
    Last modified:June 10, 2008 - v1
    Checksum:iFF59FADFE65E77FA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008937 Genomic DNA. Translation: BAG27690.1.
    RefSeqiWP_012391498.1. NC_010610.1.
    YP_001844170.1. NC_010610.1.

    Genome annotation databases

    EnsemblBacteriaiBAG27690; BAG27690; LAF_1354.
    GeneIDi6232679.
    KEGGilfe:LAF_1354.
    PATRICi22226994. VBILacFer15497_1479.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008937 Genomic DNA. Translation: BAG27690.1 .
    RefSeqi WP_012391498.1. NC_010610.1.
    YP_001844170.1. NC_010610.1.

    3D structure databases

    ProteinModelPortali B2GDF8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 334390.LAF_1354.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAG27690 ; BAG27690 ; LAF_1354 .
    GeneIDi 6232679.
    KEGGi lfe:LAF_1354.
    PATRICi 22226994. VBILacFer15497_1479.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200748.
    KOi K01869.
    OMAi DKPKYYA.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci LFER334390:GJ2S-1403-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
      Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
      DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NBRC 3956 / LMG 18251.

    Entry informationi

    Entry nameiSYL_LACF3
    AccessioniPrimary (citable) accession number: B2GDF8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3