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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei580 – 5801ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLFER334390:GJ2S-1403-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:LAF_1354
OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Taxonomic identifieri334390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001697 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 805805Leucine--tRNA ligasePRO_1000091326Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi334390.LAF_1354.

Structurei

3D structure databases

ProteinModelPortaliB2GDF8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi577 – 5815"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2GDF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYDHKAIEK KWQRYWKQHK TFKATLDKDQ KKYYALDMFP YPSGQGLHVG
60 70 80 90 100
HPEGYTATDV MSRLKRMQGF NVLHPMGWDA FGLPAEQYAL KTGHNPADFT
110 120 130 140 150
NQNVDHFRDQ IQSLGFSYDW DREVNTTDPN YYKWTQWIFE QLYKKGLAYE
160 170 180 190 200
DEIMVNWAPD FMGGTVVANE EVVDGKTERG GYPVYRVPMR QWVLKITAYA
210 220 230 240 250
DRLIDDLDLV DWPESVKEMQ RNWIGRSEGA SVKFKVVGHD GVEIEVFTTR
260 270 280 290 300
ADTLFGASYV VLAPENELVD QLTTPEQKAA VDAYKEEVSR RSDLERTELS
310 320 330 340 350
KEKTGVFTGA YVINPVNGEQ LPIWTADYVL NSYGTGAVMA VPSGDQRDFE
360 370 380 390 400
FATKFNLPIT PVVEGFNGEE AYTEDGAHVN SGFLDGLNIK EAKAKMVEWL
410 420 430 440 450
EEHDCGGKKV NYRLRDWIFS RQRYWGEPIP VIHWDDGTTS LVPEDELPLR
460 470 480 490 500
LPETDNIEPS GTGESPLANI EDWVNVYDEN GRHGKRETNT MPQWAGSSWY
510 520 530 540 550
WLRYTDPTND KEFASKEALD YWSPVDLYVG GAEHAVLHLL YARFWHKVLY
560 570 580 590 600
DLGLVPTKEP FMKLVNQGMI LGSNHEKMSK SKGNVVNPDD IVDQYGADTL
610 620 630 640 650
RLYEMFMGPL EESVPWDEKG LHGSNKWVQR VWRLLMDDNN HLRDRVSTYN
660 670 680 690 700
DGKLTKVYNQ TVKKVTDDFE RMHFNTAISQ LMVFVNEAYK VDDLPLEYMK
710 720 730 740 750
GFVKMIAPLM PHLAEELWSQ FNESETITYQ PWPTYDEKAL VEDEVEMIVQ
760 770 780 790 800
VNGKVRAKIK MAKDADNKDV EDAALANEHV HSFVDGKDVK KVIVIPNRIV

NIVVK
Length:805
Mass (Da):92,621
Last modified:June 10, 2008 - v1
Checksum:iFF59FADFE65E77FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008937 Genomic DNA. Translation: BAG27690.1.
RefSeqiWP_012391498.1. NC_010610.1.
YP_001844170.1. NC_010610.1.

Genome annotation databases

EnsemblBacteriaiBAG27690; BAG27690; LAF_1354.
GeneIDi6232679.
KEGGilfe:LAF_1354.
PATRICi22226994. VBILacFer15497_1479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008937 Genomic DNA. Translation: BAG27690.1.
RefSeqiWP_012391498.1. NC_010610.1.
YP_001844170.1. NC_010610.1.

3D structure databases

ProteinModelPortaliB2GDF8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi334390.LAF_1354.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAG27690; BAG27690; LAF_1354.
GeneIDi6232679.
KEGGilfe:LAF_1354.
PATRICi22226994. VBILacFer15497_1479.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciLFER334390:GJ2S-1403-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 3956 / LMG 18251.

Entry informationi

Entry nameiSYL_LACF3
AccessioniPrimary (citable) accession number: B2GDF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: May 27, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.