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B2GDC8

- TRMB_LACF3

UniProt

B2GDC8 - TRMB_LACF3

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, LAF_1324
Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441S-adenosyl-L-methionine By similarity
Binding sitei69 – 691S-adenosyl-L-methionine By similarity
Binding sitei96 – 961S-adenosyl-L-methionine By similarity
Active sitei118 – 1181 By similarity
Binding sitei118 – 1181S-adenosyl-L-methionine By similarity
Binding sitei122 – 1221Substrate By similarity
Binding sitei154 – 1541Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLFER334390:GJ2S-1372-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:LAF_1324
OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Taxonomic identifieri334390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001697: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
PRO_1000136352Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi334390.LAF_1324.

Structurei

3D structure databases

ProteinModelPortaliB2GDC8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1296Interaction with RNA Reviewed prediction
Regioni192 – 1954Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2GDC8-1 [UniParc]FASTAAdd to Basket

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MRVKHKKWAV PLMEAHPEMM TMDPASFKGR WQERFAKPQP LQVEVGMGKG    50
QFIIGMAKAH PEINFIGLEI ESTVAAIALK NALPEQLPNL TLVRGDGAGL 100
DTYFEDGSID RLYLNFSDPW PKTRHEKRRL TYKTFLANYQ QVVKPGGGLE 150
FKTDNQGLFE YSLTSLNNFG MIFDGVWLNL HESPENEGNV ETEYEQRFAS 200
LGQPIYKLKA HFPVN 215
Length:215
Mass (Da):24,489
Last modified:June 10, 2008 - v1
Checksum:i02A63668A7FED123
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008937 Genomic DNA. Translation: BAG27660.1.
RefSeqiYP_001844140.1. NC_010610.1.

Genome annotation databases

EnsemblBacteriaiBAG27660; BAG27660; LAF_1324.
GeneIDi6232649.
KEGGilfe:LAF_1324.
PATRICi22226932. VBILacFer15497_1449.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008937 Genomic DNA. Translation: BAG27660.1 .
RefSeqi YP_001844140.1. NC_010610.1.

3D structure databases

ProteinModelPortali B2GDC8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 334390.LAF_1324.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG27660 ; BAG27660 ; LAF_1324 .
GeneIDi 6232649.
KEGGi lfe:LAF_1324.
PATRICi 22226932. VBILacFer15497_1449.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000251689.
KOi K03439.
OMAi DNVMTEY.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci LFER334390:GJ2S-1372-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 3956 / LMG 18251.

Entry informationi

Entry nameiTRMB_LACF3
AccessioniPrimary (citable) accession number: B2GDC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: June 11, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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