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B2GDC8

- TRMB_LACF3

UniProt

B2GDC8 - TRMB_LACF3

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei44 – 441S-adenosyl-L-methionineUniRule annotation
    Binding sitei69 – 691S-adenosyl-L-methionineUniRule annotation
    Binding sitei96 – 961S-adenosyl-L-methionineUniRule annotation
    Active sitei118 – 1181By similarity
    Binding sitei118 – 1181S-adenosyl-L-methionineUniRule annotation
    Binding sitei122 – 1221SubstrateUniRule annotation
    Binding sitei154 – 1541SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciLFER334390:GJ2S-1372-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:LAF_1324
    OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
    Taxonomic identifieri334390 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000001697: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 215215tRNA (guanine-N(7)-)-methyltransferasePRO_1000136352Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi334390.LAF_1324.

    Structurei

    3D structure databases

    ProteinModelPortaliB2GDC8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1296Interaction with RNAUniRule annotation
    Regioni192 – 1954Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000251689.
    KOiK03439.
    OMAiDNVMTEY.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2GDC8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVKHKKWAV PLMEAHPEMM TMDPASFKGR WQERFAKPQP LQVEVGMGKG    50
    QFIIGMAKAH PEINFIGLEI ESTVAAIALK NALPEQLPNL TLVRGDGAGL 100
    DTYFEDGSID RLYLNFSDPW PKTRHEKRRL TYKTFLANYQ QVVKPGGGLE 150
    FKTDNQGLFE YSLTSLNNFG MIFDGVWLNL HESPENEGNV ETEYEQRFAS 200
    LGQPIYKLKA HFPVN 215
    Length:215
    Mass (Da):24,489
    Last modified:June 10, 2008 - v1
    Checksum:i02A63668A7FED123
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008937 Genomic DNA. Translation: BAG27660.1.
    RefSeqiYP_001844140.1. NC_010610.1.

    Genome annotation databases

    EnsemblBacteriaiBAG27660; BAG27660; LAF_1324.
    GeneIDi6232649.
    KEGGilfe:LAF_1324.
    PATRICi22226932. VBILacFer15497_1449.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008937 Genomic DNA. Translation: BAG27660.1 .
    RefSeqi YP_001844140.1. NC_010610.1.

    3D structure databases

    ProteinModelPortali B2GDC8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 334390.LAF_1324.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAG27660 ; BAG27660 ; LAF_1324 .
    GeneIDi 6232649.
    KEGGi lfe:LAF_1324.
    PATRICi 22226932. VBILacFer15497_1449.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000251689.
    KOi K03439.
    OMAi DNVMTEY.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci LFER334390:GJ2S-1372-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
      Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
      DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NBRC 3956 / LMG 18251.

    Entry informationi

    Entry nameiTRMB_LACF3
    AccessioniPrimary (citable) accession number: B2GDC8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3