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B2GC11 (DAPA_LACF3) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:LAF_0857
OrganismLactobacillus fermentum (strain NBRC 3956 / LMG 18251) [Complete proteome] [HAMAP]
Taxonomic identifier334390 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Dihydrodipicolinate synthase HAMAP MF_00418
PRO_1000124045

Sites

Active site1661Schiff-base intermediate with substrate By similarity
Site1381Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
B2GC11 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 2FA561A9F61E6423

FASTA31133,017
        10         20         30         40         50         60 
MTELTNADLL TAIVTPFTDE GTIDYPALDQ LTEHLLQAGN NGFVVGGTTG ETPTLSHDEK 

        70         80         90        100        110        120 
IELYTHFGRL IAGRVPVIAG TGSNNTAETI AFTNEVAQIE GIDYALVVVP PYNKPNQRGM 

       130        140        150        160        170        180 
LAHFTAVADA VELPLIIYNI PGRTGVKMET STVLTLAEHP NIAGIKQCAS LEEFQTLVEN 

       190        200        210        220        230        240 
RPAGFAVYTG EDAQALTTKV LGGDGVISVA AHNYAPQMRA MYDALSAGNY QVAAKLQRWL 

       250        260        270        280        290        300 
TPRMAALFMF PSPSPVKAVL AAQGLATDHC RLPICDLTAE EKEQLATALG LPSDALAGQL 

       310 
PTDLGEGLTN D

« Hide

References

[1]"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
DNA Res. 15:151-161(2008) [PubMed: 18487258] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBRC 3956 / LMG 18251.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008937 Genomic DNA. Translation: BAG27193.1.
RefSeqYP_001843673.1. NC_010610.1.

3D structure databases

ProteinModelPortalB2GC11.
ModBaseSearch...

Protein-protein interaction databases

STRINGB2GC11.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6232305.
GenomeReviewsGene locus LAF_0857 in contig AP008937_GR.
KEGGlfe:LAF_0857.
PATRIC22225914. VBILacFer15497_0943.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG358848.
OMARTDLAYY.
ProtClustDBPRK03170.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. False negative.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_LACF3
AccessionPrimary (citable) accession number: B2GC11
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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