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B2GBV7 (THIM_LACF3) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:LAF_0803
OrganismLactobacillus fermentum (strain NBRC 3956 / LMG 18251) [Complete proteome] [HAMAP]
Taxonomic identifier334390 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383871

Sites

Binding site401Substrate; via amide nitrogen By similarity
Binding site1161ATP By similarity
Binding site1621ATP By similarity
Binding site1891Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B2GBV7 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 3ECF8AF17DD0FEE2

FASTA25426,910
        10         20         30         40         50         60 
MQNAIATIKE TNPIVLTVAN NVTPADVANG LNALGASPMM SQTPEEADDM VNIAQAAAIN 

        70         80         90        100        110        120 
LGTLNPHQRG EMEAVMEAAK KYHKPTVLDP VACGATMYRL RVVTELLHQY HFTVIRGNAG 

       130        140        150        160        170        180 
EIATLAGVEW QSHGIDAGEG NADLEKVARL AAEKLGSVIV LSGEVDIVTD STRLARLPYG 

       190        200        210        220        230        240 
SPAFKTHVGT GDMLSSLIGA FLATNDDAFD AAVDAVTTFT LCGQAVEDQR PGNWYPGLLN 

       250 
NLDAVTDQQL AAWQ 

« Hide

References

[1]"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBRC 3956 / LMG 18251.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008937 Genomic DNA. Translation: BAG27139.1.
RefSeqYP_001843619.1. NC_010610.1.

3D structure databases

ProteinModelPortalB2GBV7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING334390.LAF_0803.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAG27139; BAG27139; LAF_0803.
GeneID6233311.
KEGGlfe:LAF_0803.
PATRIC22225798. VBILacFer15497_0885.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMASPVMAHA.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycLFER334390:GJ2S-848-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
ProtoNetSearch...

Entry information

Entry nameTHIM_LACF3
AccessionPrimary (citable) accession number: B2GBV7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 10, 2008
Last modified: February 19, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways