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B2GBV7

- THIM_LACF3

UniProt

B2GBV7 - THIM_LACF3

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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401Substrate; via amide nitrogenUniRule annotation
Binding sitei116 – 1161ATPUniRule annotation
Binding sitei162 – 1621ATPUniRule annotation
Binding sitei189 – 1891Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLFER334390:GJ2S-848-MONOMER.
UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:LAF_0803
OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Taxonomic identifieri334390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001697: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Hydroxyethylthiazole kinasePRO_0000383871Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi334390.LAF_0803.

Structurei

3D structure databases

ProteinModelPortaliB2GBV7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2145.
HOGENOMiHOG000114352.
KOiK00878.
OMAiTISESTY.
OrthoDBiEOG628F8M.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.

Sequencei

Sequence statusi: Complete.

B2GBV7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQNAIATIKE TNPIVLTVAN NVTPADVANG LNALGASPMM SQTPEEADDM
60 70 80 90 100
VNIAQAAAIN LGTLNPHQRG EMEAVMEAAK KYHKPTVLDP VACGATMYRL
110 120 130 140 150
RVVTELLHQY HFTVIRGNAG EIATLAGVEW QSHGIDAGEG NADLEKVARL
160 170 180 190 200
AAEKLGSVIV LSGEVDIVTD STRLARLPYG SPAFKTHVGT GDMLSSLIGA
210 220 230 240 250
FLATNDDAFD AAVDAVTTFT LCGQAVEDQR PGNWYPGLLN NLDAVTDQQL

AAWQ
Length:254
Mass (Da):26,910
Last modified:June 10, 2008 - v1
Checksum:i3ECF8AF17DD0FEE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008937 Genomic DNA. Translation: BAG27139.1.
RefSeqiYP_001843619.1. NC_010610.1.

Genome annotation databases

EnsemblBacteriaiBAG27139; BAG27139; LAF_0803.
GeneIDi6233311.
KEGGilfe:LAF_0803.
PATRICi22225798. VBILacFer15497_0885.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008937 Genomic DNA. Translation: BAG27139.1 .
RefSeqi YP_001843619.1. NC_010610.1.

3D structure databases

ProteinModelPortali B2GBV7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 334390.LAF_0803.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG27139 ; BAG27139 ; LAF_0803 .
GeneIDi 6233311.
KEGGi lfe:LAF_0803.
PATRICi 22225798. VBILacFer15497_0885.

Phylogenomic databases

eggNOGi COG2145.
HOGENOMi HOG000114352.
KOi K00878.
OMAi TISESTY.
OrthoDBi EOG628F8M.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
BioCyci LFER334390:GJ2S-848-MONOMER.

Family and domain databases

Gene3Di 3.40.1190.20. 1 hit.
HAMAPi MF_00228. Thz_kinase.
InterProi IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000513. Thz_kinase. 1 hit.
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF53613. SSF53613. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 3956 / LMG 18251.

Entry informationi

Entry nameiTHIM_LACF3
AccessioniPrimary (citable) accession number: B2GBV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 10, 2008
Last modified: November 26, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3