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B2GBV7

- THIM_LACF3

UniProt

B2GBV7 - THIM_LACF3

Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

    Catalytic activityi

    ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401Substrate; via amide nitrogenUniRule annotation
    Binding sitei116 – 1161ATPUniRule annotation
    Binding sitei162 – 1621ATPUniRule annotation
    Binding sitei189 – 1891Substrate; via amide nitrogenUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. hydroxyethylthiazole kinase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-KW
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciLFER334390:GJ2S-848-MONOMER.
    UniPathwayiUPA00060; UER00139.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
    Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
    Short name:
    TH kinaseUniRule annotation
    Short name:
    Thz kinaseUniRule annotation
    Gene namesi
    Name:thiMUniRule annotation
    Ordered Locus Names:LAF_0803
    OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
    Taxonomic identifieri334390 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000001697: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 254254Hydroxyethylthiazole kinasePRO_0000383871Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi334390.LAF_0803.

    Structurei

    3D structure databases

    ProteinModelPortaliB2GBV7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Thz kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2145.
    HOGENOMiHOG000114352.
    KOiK00878.
    OMAiTISESTY.
    OrthoDBiEOG628F8M.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    HAMAPiMF_00228. Thz_kinase.
    InterProiIPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF02110. HK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000513. Thz_kinase. 1 hit.
    PRINTSiPR01099. HYETHTZKNASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B2GBV7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQNAIATIKE TNPIVLTVAN NVTPADVANG LNALGASPMM SQTPEEADDM    50
    VNIAQAAAIN LGTLNPHQRG EMEAVMEAAK KYHKPTVLDP VACGATMYRL 100
    RVVTELLHQY HFTVIRGNAG EIATLAGVEW QSHGIDAGEG NADLEKVARL 150
    AAEKLGSVIV LSGEVDIVTD STRLARLPYG SPAFKTHVGT GDMLSSLIGA 200
    FLATNDDAFD AAVDAVTTFT LCGQAVEDQR PGNWYPGLLN NLDAVTDQQL 250
    AAWQ 254
    Length:254
    Mass (Da):26,910
    Last modified:June 10, 2008 - v1
    Checksum:i3ECF8AF17DD0FEE2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008937 Genomic DNA. Translation: BAG27139.1.
    RefSeqiYP_001843619.1. NC_010610.1.

    Genome annotation databases

    EnsemblBacteriaiBAG27139; BAG27139; LAF_0803.
    GeneIDi6233311.
    KEGGilfe:LAF_0803.
    PATRICi22225798. VBILacFer15497_0885.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008937 Genomic DNA. Translation: BAG27139.1 .
    RefSeqi YP_001843619.1. NC_010610.1.

    3D structure databases

    ProteinModelPortali B2GBV7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 334390.LAF_0803.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAG27139 ; BAG27139 ; LAF_0803 .
    GeneIDi 6233311.
    KEGGi lfe:LAF_0803.
    PATRICi 22225798. VBILacFer15497_0885.

    Phylogenomic databases

    eggNOGi COG2145.
    HOGENOMi HOG000114352.
    KOi K00878.
    OMAi TISESTY.
    OrthoDBi EOG628F8M.

    Enzyme and pathway databases

    UniPathwayi UPA00060 ; UER00139 .
    BioCyci LFER334390:GJ2S-848-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    HAMAPi MF_00228. Thz_kinase.
    InterProi IPR000417. Hyethyz_kinase.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    Pfami PF02110. HK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000513. Thz_kinase. 1 hit.
    PRINTSi PR01099. HYETHTZKNASE.
    SUPFAMi SSF53613. SSF53613. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
      Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
      DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NBRC 3956 / LMG 18251.

    Entry informationi

    Entry nameiTHIM_LACF3
    AccessioniPrimary (citable) accession number: B2GBV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3