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B2GBT4 (B2GBT4_LACF3) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-galactosidase PIRNR PIRNR001084
Short name=Beta-gal PIRNR PIRNR001084
EC=3.2.1.23 PIRNR PIRNR001084
Gene names
Ordered Locus Names:LAF_0780
OrganismLactobacillus fermentum (strain NBRC 3956 / LMG 18251) [Complete proteome] [HAMAP]
Taxonomic identifier334390 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length686 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. PIRNR PIRNR001084

Sequence similarities

Belongs to the glycosyl hydrolase 42 family. PIRNR PIRNR001084

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1531Proton donor By similarity PIRSR PIRSR001084-1
Active site3171Nucleophile By similarity PIRSR PIRSR001084-1
Metal binding1181Zinc By similarity PIRSR PIRSR001084-3
Metal binding1581Zinc By similarity PIRSR PIRSR001084-3
Metal binding1601Zinc By similarity PIRSR PIRSR001084-3
Metal binding1631Zinc By similarity PIRSR PIRSR001084-3
Binding site1141Substrate By similarity PIRSR PIRSR001084-2
Binding site1521Substrate By similarity PIRSR PIRSR001084-2
Binding site3251Substrate By similarity PIRSR PIRSR001084-2

Sequences

Sequence LengthMass (Da)Tools
B2GBT4 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 347DBA06D1D556E7

FASTA68679,664
        10         20         30         40         50         60 
MTDKLRLSHF LHGGDYNPDQ WLDHPEVIDK DFKLFKEAKI NTVSLGIFAW SKLEPEEGVF 

        70         80         90        100        110        120 
DFEWLDNIFD RVEEQGGNII LATPSGAKPR WLAEKYPEVL RVNEKNERNI FGERHNHCYT 

       130        140        150        160        170        180 
SPIYREKVQE INTKLAERYG KRKSLLMWHI SNEYGGQCFC DLCQEKFRTW MKNKYKSLDN 

       190        200        210        220        230        240 
LNKAYWSSFW SHTYTSWDQV HSPSTLGDST FLGLNLDWKR FVTERTIDFF EVEIKPLRKL 

       250        260        270        280        290        300 
TPEIPITTNF MGGNPPDSHV FYDLDYQEFA KHVDVVSWDS YPNWGNDYES TAQLGMKTAL 

       310        320        330        340        350        360 
MNDMMRSLKH DNYLIMESTP SQVNWHPFNR SKRPGMHEMG SLQQVAHGAD SALYFQLHQS 

       370        380        390        400        410        420 
RGASEMYHGA VITHTMNDKT RAFHDVTTVG KDLEKLQGAL STKHQPAKVA IVFDYDNMWA 

       430        440        450        460        470        480 
LDDSRNYADS TKKYWRTIQQ HYQYFWEHDV PVEFVSVKDD LTPYTLVIDP MHFMMAPEFS 

       490        500        510        520        530        540 
TKLHDYVEQG GHLVGTYITG MVDKQFLAYL HGWPKTLHET YGIEFTETDT LYPKQHNQLI 

       550        560        570        580        590        600 
YNSKKFVVSD YCDLFTVDTA EEIGQYGSDF YAGTSALTKN KLGSGTAFYI GCRTDTDFLA 

       610        620        630        640        650        660 
DFYGQLTIQL GLIDSKPITK DSNKVSVQVR RNDKSNYYFA MNFSHVESAI VVNEPVYDVL 

       670        680 
NGHMIDRGPQ KISPYGVNVY REDNTK

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References

[1]"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
DNA Res. 15:151-161(2008) [PubMed: 18487258] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBRC 3956 / LMG 18251.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008937 Genomic DNA. Translation: BAG27116.1.
RefSeqYP_001843596.1. NC_010610.1.

3D structure databases

ProteinModelPortalB2GBT4.
ModBaseSearch...

Protein-protein interaction databases

STRINGB2GBT4.

Protein family/group databases

CAZyGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6233288.
GenomeReviewsGene locus LAF_0780 in contig AP008937_GR.
KEGGlfe:LAF_0780.
PATRIC22225740. VBILacFer15497_0856.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG476453.
OMAAKRPGMH.
ProtClustDBCLSK582025.

Family and domain databases

InterProIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KOK12308.
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB2GBT4_LACF3
AccessionPrimary (citable) accession number: B2GBT4
Entry history
Integrated into UniProtKB/TrEMBL: June 10, 2008
Last sequence update: June 10, 2008
Last modified: January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info