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B2GB86

- SYI_LACF3

UniProt

B2GB86 - SYI_LACF3

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei555 – 5551Aminoacyl-adenylateUniRule annotation
    Binding sitei599 – 5991ATPUniRule annotation
    Metal bindingi889 – 8891ZincUniRule annotation
    Metal bindingi892 – 8921ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi912 – 9121ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciLFER334390:GJ2S-626-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:LAF_0582
    OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
    Taxonomic identifieri334390 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
    ProteomesiUP000001697: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 930930Isoleucine--tRNA ligasePRO_1000189175Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi334390.LAF_0582.

    Structurei

    3D structure databases

    ProteinModelPortaliB2GB86.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi596 – 6005"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGRRSCQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2GB86-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRVKDTLNLG KTKFPMRGRL PETEAQREAL WEENKVYEQR QKLNEGKPSF    50
    VLHDGPPYAN GPIHIGHAMN KISKDFIVRY KSMTGYRAPY VPGWDTHGLP 100
    IEHQLTKAGY DRKKMSLTEF RDLCQKYALE QVEIQKKGFK RLGVAGEWDH 150
    PYLTLAKEFE AAQIKVFGAF AKRGLLYQAK KPVYWSWSSE SALAEAEVEY 200
    HDVVAKTAFF TEQVQDGKGL LDSDTYLVGW TTTPWTIPAS EAVAVSADFE 250
    YALVQPSGSD RKYVVAASLL GDLAQKFNWT DYQVVKTFKG AEMEGMTTKH 300
    PYIDRELLVG LADYVTDDAG TGLVHTAPGY GDDDYNFGKK YNLPIFAPMN 350
    DQGVLTAENG PEFDGVFYQD ADDISLRLLE EHDALLLEED LEHSYPFDWR 400
    TKKPIVFRAT DQWFVSIDKM RDEILKAVDE VTYYPTWGKV RLRNMLKDRG 450
    DWVISRQRVW GVPLPIFYAE DGTPIMTEET INHVSDLFRE YGSNVWFDRE 500
    AKDLLPAGFT SEHSPNGKFT KETDIMDVWF DSGSSHQGVL AERDYLTYPA 550
    DMYLEGSDQY RGWFNSSLIT SVVVSGHAPY KSVLSQGFTL DQSGKKMSKS 600
    LGNVIDPNKV VKQMGAEIIR LWVMSADTSA DVRVSMETLQ QISESYRKLR 650
    NTFRFLLANT SDFGPENFVA YEKREAVDQY MTVNFNHFLA GMRDEFDRYD 700
    FLNAYKHLIN FVNNDLSSFY MNVAKDVLYI EPEDSHVRRS MQATFYEILS 750
    GLTKLLTPIL PHTTEEVWSY MDEPEDFVQL TEIPEARTFE NGNALLEKWE 800
    GFMELRSHVL KCLEEARNAK LIGRSLEASA DLYLTASQQE LLADLGTDAG 850
    LLCGVSALSV HDASEAPAEA ESFSDNAAVL VQAAKGEVCD RCRMTKEDVG 900
    SDPAYQQLCA RCAKLVRENF PQTVEEGLEK 930
    Length:930
    Mass (Da):105,635
    Last modified:June 10, 2008 - v1
    Checksum:iA87228F73E2C1A54
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008937 Genomic DNA. Translation: BAG26918.1.
    RefSeqiYP_001843398.1. NC_010610.1.

    Genome annotation databases

    EnsemblBacteriaiBAG26918; BAG26918; LAF_0582.
    GeneIDi6233688.
    KEGGilfe:LAF_0582.
    PATRICi22225282. VBILacFer15497_0628.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008937 Genomic DNA. Translation: BAG26918.1 .
    RefSeqi YP_001843398.1. NC_010610.1.

    3D structure databases

    ProteinModelPortali B2GB86.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 334390.LAF_0582.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAG26918 ; BAG26918 ; LAF_0582 .
    GeneIDi 6233688.
    KEGGi lfe:LAF_0582.
    PATRICi 22225282. VBILacFer15497_0628.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGRRSCQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci LFER334390:GJ2S-626-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
      Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
      DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NBRC 3956 / LMG 18251.

    Entry informationi

    Entry nameiSYI_LACF3
    AccessioniPrimary (citable) accession number: B2GB86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3