B2GAM7 (UNG_LACF3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil-DNA glycosylase
Short name=UDG
EC=3.2.2.27

Gene names

Name:	ung
Ordered Locus Names:	LAF_0373

Organism

Lactobacillus fermentum (strain NBRC 3956 / LMG 18251) [Complete proteome] [HAMAP]

Taxonomic identifier

334390 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus fermentum

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine By similarity. HAMAP-Rule MF_00148
Catalytic activity	Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil. HAMAP-Rule MF_00148
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the uracil-DNA glycosylase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	uracil DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Uracil-DNA glycosylase HAMAP-Rule MF_00148		PRO_1000096589
Sites
	Active site	65	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2GAM7 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: A06781A527E3AB6E	FASTA	229	25,937
	10 20 30 40 50 60 MKQLINNDWW PVLKPQFETA NYQQLHNFLV DEYGHQQVYP EMHHIFEAFN WTPFSKVKVV 70 80 90 100 110 120 ILGQDPYHGP GQAHGCSFSV LPGVAVPPSL QNIYKELQAD LGCPPVKHGY LRSWAEQGVL 130 140 150 160 170 180 LLNSVLTVRA GQAYSHQGHG WEQLTDAAIV ALSKRPTPVV FILWGRAARD KKRLIDLKRN 190 200 210 220 FVVESAHPSP LSAYRGFFGS RPFSKTNQFL EMTGQAPINW QLPSTVDHL

References

[1]

"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production." Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M. DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NBRC 3956 / LMG 18251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008937 Genomic DNA. Translation: BAG26709.1.
RefSeq	YP_001843189.1. NC_010610.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	334390.LAF_0373.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAG26709; BAG26709; LAF_0373.
GeneID	6232424.
KEGG	lfe:LAF_0373.
PATRIC	22224790. VBILacFer15497_0406.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0692.
HOGENOM	HOG000229528.
KO	K03648.
OMA	AGKEIYP.
ProtClustDB	PRK05254.
Enzyme and pathway databases
BioCyc	LFER334390:GJ2S-374-MONOMER.
Family and domain databases
Gene3D	3.40.470.10. 1 hit.
HAMAP	MF_00148. UDG.
InterPro	IPR018085. Ura-DNA_Glyclase_AS. IPR002043. Ura_DNA_glycsylse. IPR005122. Uracil-DNA_glycosylase-like. [Graphical view]
PANTHER	PTHR11264. PTHR11264. 1 hit.
Pfam	PF03167. UDG. 1 hit. [Graphical view]
SMART	SM00986. UDG. 1 hit. [Graphical view]
SUPFAM	SSF52141. UDNA_glycsylseSF. 1 hit.
TIGRFAMs	TIGR00628. ung. 1 hit.
PROSITE	PS00130. U_DNA_GLYCOSYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UNG_LACF3

Accession

Primary (citable) accession number: B2GAM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program