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B2GAB3

- SYE_LACF3

UniProt

B2GAB3 - SYE_LACF3

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Protein

Glutamate--tRNA ligase

Gene
gltX, LAF_0259
Organism
Lactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei262 – 2621ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLFER334390:GJ2S-279-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:LAF_0259
OrganismiLactobacillus fermentum (strain NBRC 3956 / LMG 18251)
Taxonomic identifieri334390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000001697: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498Glutamate--tRNA ligaseUniRule annotationPRO_1000090083Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi334390.LAF_0259.

Structurei

3D structure databases

ProteinModelPortaliB2GAB3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2211"HIGH" regionUniRule annotationAdd
BLAST
Motifi259 – 2635"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK09698.
OMAiKHYDGDF.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2GAB3-1 [UniParc]FASTAAdd to Basket

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MSKNEIRVRY APSPTGHLHI GNARTALFNY LFARHNHGKF IIRIEDTDTK    50
RNIADGERSQ LDNLKWMGLD WDEGPDKGGD FGPYRQSERK DIYAQYIQEL 100
MDKGLAYKSY MTEEELEAQR EAQKAAHQMP HYEYEYAGMS DDQIKAAQEA 150
AEEKGLKPVI RFRVPKDEVF EWEDLVKGPM SFEAQSIGGD FVIQKRDGMP 200
TYNFAVVIDD HLMKISHVFR GDDHVSNTPK QMAIYQALGW KVPEFGHMSL 250
IINNETGKKL SKRDESVLQF IEQYRDLGYL PEAMDNFIIL LGWSPVGEDE 300
IFSLKEFVKM YDEKRLSKSP AAFDRKKLQW INNQYMKLSS ADEVFHVAMP 350
QLLDAGLIEK NANPYKMEWM RRLVELFKRE ISYAREIVDY VKPFVNGPED 400
ISEEAKAEMQ EDTALVVIKA FRDRVAAMDF MDATGVLAAI KDVQKSTKVK 450
GRKLWMPLRI AVTHETHGPE LPESIELFGQ EKTLAHLDEM IAQLEENK 498
Length:498
Mass (Da):57,508
Last modified:June 10, 2008 - v1
Checksum:i207FF7F3D3E922A3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008937 Genomic DNA. Translation: BAG26595.1.
RefSeqiYP_001843075.1. NC_010610.1.

Genome annotation databases

EnsemblBacteriaiBAG26595; BAG26595; LAF_0259.
GeneIDi6233363.
KEGGilfe:LAF_0259.
PATRICi22224554. VBILacFer15497_0290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008937 Genomic DNA. Translation: BAG26595.1 .
RefSeqi YP_001843075.1. NC_010610.1.

3D structure databases

ProteinModelPortali B2GAB3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 334390.LAF_0259.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG26595 ; BAG26595 ; LAF_0259 .
GeneIDi 6233363.
KEGGi lfe:LAF_0259.
PATRICi 22224554. VBILacFer15497_0290.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K09698.
OMAi KHYDGDF.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci LFER334390:GJ2S-279-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 3956 / LMG 18251.

Entry informationi

Entry nameiSYE_LACF3
AccessioniPrimary (citable) accession number: B2GAB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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