B2GA98 (NADE_LACF3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	LAF_0244

Organism

Lactobacillus fermentum (strain NBRC 3956 / LMG 18251) [Complete proteome] [HAMAP]

Taxonomic identifier

334390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099029
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2GA98 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 19D04278F2AA0F1C	FASTA	276	30,854
	10 20 30 40 50 60 MRQLQKQIIE DLKVIPTIDP QVEVRRRIDF LKDYLKQTKM ATLVLGISGG QDSALAGRLA 70 80 90 100 110 120 QLAVEELRKE SGSEDYQFIA VRLPYGEQAD ESDAMMAIDD FIHPDRVVKV NIKPATDAMV 130 140 150 160 170 180 MTLEAAGTKI SDFNKGNIKA RERMIVQYAI AGEYHGAVVG TDHAAEAVTG FYTKYGDGGA 190 200 210 220 230 240 DVTPLSQLDK RQGRALLEYL GAPEKLYQKT PTADLEEDRP ALPDEQALGV TYKDIDDFLE 250 260 270 GREVDQAAAE KIEAWYQRTG HKRHMPVAPL DTWWKD

References

[1]

"Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production." Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M. DNA Res. 15:151-161(2008) [PubMed: 18487258] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NBRC 3956 / LMG 18251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008937 Genomic DNA. Translation: BAG26580.1.
RefSeq	YP_001843060.1. NC_010610.1.
3D structure databases
ProteinModelPortal	B2GA98.
ModBase	Search...
Protein-protein interaction databases
STRING	B2GA98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6233348.
GenomeReviews	Gene locus LAF_0244 in contig AP008937_GR.
KEGG	lfe:LAF_0244.
PATRIC	22224522. VBILacFer15497_0275.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	GLNKRRV.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_LACF3

Accession

Primary (citable) accession number: B2GA98

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program