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B2G8G2

- SYL_LACRJ

UniProt

B2G8G2 - SYL_LACRJ

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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lactobacillus reuteri (strain JCM 1112)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLREU557433:GHNR-1291-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:LAR_1228
OrganismiLactobacillus reuteri (strain JCM 1112)
Taxonomic identifieri557433 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000009150: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 806806Leucine--tRNA ligasePRO_1000091330Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi557433.LAR_1228.

Structurei

3D structure databases

ProteinModelPortaliB2G8G2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi578 – 5825"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiDKPKYYA.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2G8G2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYDHKTIEK KWQKFWKKNE TFKADLNKDQ KKYYALDMFP YPSGQGLHVG
60 70 80 90 100
HPEGYTATDV MSRMKRMQGF NVLHPMGWDA FGLPAEQYAL KTGHNPKDFT
110 120 130 140 150
NKNIDHFRDQ IQSLGFSYDW DREVNTTDPK FYKWTQWIFE QLYKKGLAYE
160 170 180 190 200
SEIMVNWAPD FMGGTVVANE EVEDGKTKRG GYPVYRKPMR QWVLKITAYA
210 220 230 240 250
DRLIDDLDLV DWPESVKEMQ RNWIGRSEGA SVFFPVVGDE DTKIEVFTTR
260 270 280 290 300
ADTLFGASYV VLAPEQELVD QLTTPEHKAE VEKYKEEASR RSDLERTDLN
310 320 330 340 350
KDKTGVFTGS YVINPVNGEK LPIWISDYVL ASYGTGAVMA VPSGDQRDYD
360 370 380 390 400
FATKFNLPIK PIIEGADISE GAFDGDGKHI NSGFLDGLNI ADAKQKMIDW
410 420 430 440 450
LEEHDAGHKK VNYRLRDWIF SRQRYWGEPI PVIHWDDGTT SLVPEDELPL
460 470 480 490 500
ELPKTDNIEP SGTGESPLAN VEDWVNVYDE NGRHGLRETN TMPQWAGSSW
510 520 530 540 550
YWLRYTDPHN DEEFASKEAL DYWSPVDLYV GGAEHAVLHL LYARFWHKVL
560 570 580 590 600
YDLGLVPTKE PFMKLVNQGM ILGSNHEKMS KSKGNVVNPD DIVDQYGADT
610 620 630 640 650
LRLYEMFMGP LEESVPWDEK GLHGANKWVQ RVWRLLMDDN NHLRDRVSTF
660 670 680 690 700
NDGKLTKVYN QTVKKVTEDY ERMHFNTAIS QLMVFVNEAY KVDDLPVEYM
710 720 730 740 750
KGFVKMIAPI MPHMAEELWS QFGESDTITY QPWPTYDPKA LVEDEVEMIV
760 770 780 790 800
QVNGKVRAKI KMAKDTDRDE AQQLALANEH VKKFTDGKDI KKVIVVPNKI

VNIVAK
Length:806
Mass (Da):92,919
Last modified:June 10, 2008 - v1
Checksum:i08383790E3207E6C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP007281 Genomic DNA. Translation: BAG25744.1.
RefSeqiYP_001842224.1. NC_010609.1.

Genome annotation databases

EnsemblBacteriaiBAG25744; BAG25744; LAR_1228.
GeneIDi6231131.
KEGGilrf:LAR_1228.
PATRICi22259984. VBILacReu111271_1346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP007281 Genomic DNA. Translation: BAG25744.1 .
RefSeqi YP_001842224.1. NC_010609.1.

3D structure databases

ProteinModelPortali B2G8G2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 557433.LAR_1228.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG25744 ; BAG25744 ; LAR_1228 .
GeneIDi 6231131.
KEGGi lrf:LAR_1228.
PATRICi 22259984. VBILacReu111271_1346.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200748.
KOi K01869.
OMAi DKPKYYA.
OrthoDBi EOG63Z74X.

Enzyme and pathway databases

BioCyci LREU557433:GHNR-1291-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 1112.

Entry informationi

Entry nameiSYL_LACRJ
AccessioniPrimary (citable) accession number: B2G8G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3