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B2G868

- SYE_LACRJ

UniProt

B2G868 - SYE_LACRJ

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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Lactobacillus reuteri (strain JCM 1112)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei260 – 2601ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLREU557433:GHNR-1195-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:LAR_1134
OrganismiLactobacillus reuteri (strain JCM 1112)
Taxonomic identifieri557433 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000009150: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Glutamate--tRNA ligasePRO_1000090084Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi557433.LAR_1134.

Structurei

3D structure databases

ProteinModelPortaliB2G868.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi257 – 2615"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK09698.
OMAiTISPIFH.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2G868 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDQKVRVRYA PSPTGFLHIG NAQSALFNYL FARHFDGTMV LRIEDTDTKR
60 70 80 90 100
NVEDGEASQR ENLHWLGIDW DEGPNKPNPK YAPYRQSERN KEGIYHKYIQ
110 120 130 140 150
ELLDKGIAYK DYSTEEELAE MRERQKANNE PPHYDGRWYG KSEEEQKAAE
160 170 180 190 200
AKGLKPTIRF HFPKDHDYEW DDIARGHVSF NSDNLGGDFI IEKSDGMPTY
210 220 230 240 250
NFAVVVDDHT MDITHVLRGA DHISNTPKQI AIYEALGWEH PTFCHIPLIF
260 270 280 290 300
NPKTRKKLSK RDKDTLQFIS EYKKHGYLHE AIFNFIAFLG WSPVGEREIY
310 320 330 340 350
SKEELIKVYD PKRMSKAPAY FDQKKLDWMN AQYIKSMSID ELTDRTMELI
360 370 380 390 400
KEGETEEAKR LQSIPEEQLT ELLKKTIKVH QRDVNKLLEV IQYAWSYYTV
410 420 430 440 450
LDQSFNYDLL KDNEDFANED VLAVLKGLKA KLENGDDDLD YSQAIKEVGK
460 470 480 490 500
DTGIKGRGLY FPLNLAFTGS TSAPQIYEIM DIYSRDTDIE LLDRMIKAFE

N
Length:501
Mass (Da):58,350
Last modified:June 10, 2008 - v1
Checksum:i299495BBD03FBE6A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP007281 Genomic DNA. Translation: BAG25650.1.
RefSeqiYP_001842130.1. NC_010609.1.

Genome annotation databases

EnsemblBacteriaiBAG25650; BAG25650; LAR_1134.
GeneIDi6231269.
KEGGilrf:LAR_1134.
PATRICi22259788. VBILacReu111271_1250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP007281 Genomic DNA. Translation: BAG25650.1 .
RefSeqi YP_001842130.1. NC_010609.1.

3D structure databases

ProteinModelPortali B2G868.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 557433.LAR_1134.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG25650 ; BAG25650 ; LAR_1134 .
GeneIDi 6231269.
KEGGi lrf:LAR_1134.
PATRICi 22259788. VBILacReu111271_1250.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K09698.
OMAi TISPIFH.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci LREU557433:GHNR-1195-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 1112.

Entry informationi

Entry nameiSYE_LACRJ
AccessioniPrimary (citable) accession number: B2G868
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3