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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus reuteri (strain JCM 1112)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei555Aminoacyl-adenylateUniRule annotation1
Binding sitei599ATPUniRule annotation1
Metal bindingi890ZincUniRule annotation1
Metal bindingi893ZincUniRule annotation1
Metal bindingi910ZincUniRule annotation1
Metal bindingi913ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LAR_0578
OrganismiLactobacillus reuteri (strain JCM 1112)
Taxonomic identifieri557433 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891761 – 931Isoleucine--tRNA ligaseAdd BLAST931

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB2G6L2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi596 – 600"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B2G6L2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVKDTLNLG RTKFPMRGKL PVTEAQREQL WEENKVYELR QKLNEGKPTF
60 70 80 90 100
VLHDGPPYAN GNIHIGHAMN KISKDFIVRY KSMSGYRAPY VPGWDTHGLP
110 120 130 140 150
IEHQLTKSGY DRKKMSLTEF RDLCREYALK QVDKQRTDFK RLGVSGEWDH
160 170 180 190 200
PYLTLDKEFE AAQIRVFGEF AKKGLLYQAK KPVYWSWSSE SALAEAEVEY
210 220 230 240 250
HDVVAKTAFF VEQIKDGKGR LDNDTYLVVW TTTPWTVPAS EAVAVNPKFD
260 270 280 290 300
YSVVNPANDD RKFVVATDLL EKLAEKLGWE DYEVVDHLMG QELEGMTTQH
310 320 330 340 350
PYLDRDLLVG LADYVTADAG TGLVHTAPGY GDDDYNFGKK YDLPIFAPIN
360 370 380 390 400
DQGVLTKENG EGFEGVFYQD ADDVSLQKLE ENNALLLQEP LQHSYPFDWR
410 420 430 440 450
TKQPIIFRAT DQWFVSIEKM RQNILDALED VKYHPEWGKV RLRNMIKDRG
460 470 480 490 500
DWVISRQRVW GVPLPIFYAE DGTPIMEEET INHVAELFAK YGSNVWFERE
510 520 530 540 550
AKDLLPEGYT NEHSPNGKFT KETDIMDVWF DSGSSHQGVL AERDYLTYPA
560 570 580 590 600
DLYLEGSDQY RGWFNSSLIT SVVCSGHAPY KEIVSQGFTL DKRGNKMSKS
610 620 630 640 650
QGNVIDPNKV VQQMGAEIIR LWVMSADTSA DVRVSMGTFQ QISEAYRKLR
660 670 680 690 700
NTFRFLLANT SDFNPEENTV SYEKLQSVDK YMLVKLNHFL KTMREDFDNY
710 720 730 740 750
DFLDAYKVLI NFVNNDLSAF YMNIAKDVLY IEAENSEVRR SMQTVFYDIL
760 770 780 790 800
LTLVKLLTPI LPHTTEEVWS YMNEPEDFVQ LTEIPDPRTF AGEEELLSKW
810 820 830 840 850
DDFMEVRSHV LKSLEEARNA KLIGKSLEAQ VDLYLTDDQK QLLDSLNENI
860 870 880 890 900
QLLLGVSALH VHPADEAPAD ADQYNDGVAV KVTTANGETC ARCRMVKEDV
910 920 930
GSDPAYPELC ARCAAIVREN FPETAEDGLE E
Length:931
Mass (Da):106,698
Last modified:June 10, 2008 - v1
Checksum:i187428AD377459BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP007281 Genomic DNA Translation: BAG25094.1
RefSeqiWP_012390521.1, NC_010609.1

Genome annotation databases

EnsemblBacteriaiBAG25094; BAG25094; LAR_0578
GeneIDi5189657
KEGGilrf:LAR_0578

Similar proteinsi

Entry informationi

Entry nameiSYI_LACRJ
AccessioniPrimary (citable) accession number: B2G6L2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: March 28, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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