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B2G6L2

- SYI_LACRJ

UniProt

B2G6L2 - SYI_LACRJ

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Protein
Isoleucine--tRNA ligase
Gene
ileS, LAR_0578
Organism
Lactobacillus reuteri (strain JCM 1112)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei555 – 5551Aminoacyl-adenylate By similarity
Binding sitei599 – 5991ATP By similarity
Metal bindingi890 – 8901Zinc By similarity
Metal bindingi893 – 8931Zinc By similarity
Metal bindingi910 – 9101Zinc By similarity
Metal bindingi913 – 9131Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLREU557433:GHNR-625-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:LAR_0578
OrganismiLactobacillus reuteri (strain JCM 1112)
Taxonomic identifieri557433 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000009150: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189176Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi557433.LAR_0578.

Structurei

3D structure databases

ProteinModelPortaliB2G6L2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi596 – 6005"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2G6L2-1 [UniParc]FASTAAdd to Basket

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MRVKDTLNLG RTKFPMRGKL PVTEAQREQL WEENKVYELR QKLNEGKPTF    50
VLHDGPPYAN GNIHIGHAMN KISKDFIVRY KSMSGYRAPY VPGWDTHGLP 100
IEHQLTKSGY DRKKMSLTEF RDLCREYALK QVDKQRTDFK RLGVSGEWDH 150
PYLTLDKEFE AAQIRVFGEF AKKGLLYQAK KPVYWSWSSE SALAEAEVEY 200
HDVVAKTAFF VEQIKDGKGR LDNDTYLVVW TTTPWTVPAS EAVAVNPKFD 250
YSVVNPANDD RKFVVATDLL EKLAEKLGWE DYEVVDHLMG QELEGMTTQH 300
PYLDRDLLVG LADYVTADAG TGLVHTAPGY GDDDYNFGKK YDLPIFAPIN 350
DQGVLTKENG EGFEGVFYQD ADDVSLQKLE ENNALLLQEP LQHSYPFDWR 400
TKQPIIFRAT DQWFVSIEKM RQNILDALED VKYHPEWGKV RLRNMIKDRG 450
DWVISRQRVW GVPLPIFYAE DGTPIMEEET INHVAELFAK YGSNVWFERE 500
AKDLLPEGYT NEHSPNGKFT KETDIMDVWF DSGSSHQGVL AERDYLTYPA 550
DLYLEGSDQY RGWFNSSLIT SVVCSGHAPY KEIVSQGFTL DKRGNKMSKS 600
QGNVIDPNKV VQQMGAEIIR LWVMSADTSA DVRVSMGTFQ QISEAYRKLR 650
NTFRFLLANT SDFNPEENTV SYEKLQSVDK YMLVKLNHFL KTMREDFDNY 700
DFLDAYKVLI NFVNNDLSAF YMNIAKDVLY IEAENSEVRR SMQTVFYDIL 750
LTLVKLLTPI LPHTTEEVWS YMNEPEDFVQ LTEIPDPRTF AGEEELLSKW 800
DDFMEVRSHV LKSLEEARNA KLIGKSLEAQ VDLYLTDDQK QLLDSLNENI 850
QLLLGVSALH VHPADEAPAD ADQYNDGVAV KVTTANGETC ARCRMVKEDV 900
GSDPAYPELC ARCAAIVREN FPETAEDGLE E 931
Length:931
Mass (Da):106,698
Last modified:June 10, 2008 - v1
Checksum:i187428AD377459BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP007281 Genomic DNA. Translation: BAG25094.1.
RefSeqiWP_012390521.1. NC_010609.1.
YP_001841574.1. NC_010609.1.

Genome annotation databases

EnsemblBacteriaiBAG25094; BAG25094; LAR_0578.
GeneIDi6230512.
KEGGilrf:LAR_0578.
PATRICi22258491. VBILacReu111271_0618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP007281 Genomic DNA. Translation: BAG25094.1 .
RefSeqi WP_012390521.1. NC_010609.1.
YP_001841574.1. NC_010609.1.

3D structure databases

ProteinModelPortali B2G6L2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 557433.LAR_0578.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG25094 ; BAG25094 ; LAR_0578 .
GeneIDi 6230512.
KEGGi lrf:LAR_0578.
PATRICi 22258491. VBILacReu111271_0618.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LREU557433:GHNR-625-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 1112.

Entry informationi

Entry nameiSYI_LACRJ
AccessioniPrimary (citable) accession number: B2G6L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: September 3, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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