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B2G6L2

- SYI_LACRJ

UniProt

B2G6L2 - SYI_LACRJ

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Lactobacillus reuteri (strain JCM 1112)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei555 – 5551Aminoacyl-adenylateUniRule annotation
Binding sitei599 – 5991ATPUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi893 – 8931ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi913 – 9131ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLREU557433:GHNR-625-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:LAR_0578
OrganismiLactobacillus reuteri (strain JCM 1112)
Taxonomic identifieri557433 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000009150: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Isoleucine--tRNA ligasePRO_1000189176Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi557433.LAR_0578.

Structurei

3D structure databases

ProteinModelPortaliB2G6L2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi596 – 6005"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2G6L2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVKDTLNLG RTKFPMRGKL PVTEAQREQL WEENKVYELR QKLNEGKPTF
60 70 80 90 100
VLHDGPPYAN GNIHIGHAMN KISKDFIVRY KSMSGYRAPY VPGWDTHGLP
110 120 130 140 150
IEHQLTKSGY DRKKMSLTEF RDLCREYALK QVDKQRTDFK RLGVSGEWDH
160 170 180 190 200
PYLTLDKEFE AAQIRVFGEF AKKGLLYQAK KPVYWSWSSE SALAEAEVEY
210 220 230 240 250
HDVVAKTAFF VEQIKDGKGR LDNDTYLVVW TTTPWTVPAS EAVAVNPKFD
260 270 280 290 300
YSVVNPANDD RKFVVATDLL EKLAEKLGWE DYEVVDHLMG QELEGMTTQH
310 320 330 340 350
PYLDRDLLVG LADYVTADAG TGLVHTAPGY GDDDYNFGKK YDLPIFAPIN
360 370 380 390 400
DQGVLTKENG EGFEGVFYQD ADDVSLQKLE ENNALLLQEP LQHSYPFDWR
410 420 430 440 450
TKQPIIFRAT DQWFVSIEKM RQNILDALED VKYHPEWGKV RLRNMIKDRG
460 470 480 490 500
DWVISRQRVW GVPLPIFYAE DGTPIMEEET INHVAELFAK YGSNVWFERE
510 520 530 540 550
AKDLLPEGYT NEHSPNGKFT KETDIMDVWF DSGSSHQGVL AERDYLTYPA
560 570 580 590 600
DLYLEGSDQY RGWFNSSLIT SVVCSGHAPY KEIVSQGFTL DKRGNKMSKS
610 620 630 640 650
QGNVIDPNKV VQQMGAEIIR LWVMSADTSA DVRVSMGTFQ QISEAYRKLR
660 670 680 690 700
NTFRFLLANT SDFNPEENTV SYEKLQSVDK YMLVKLNHFL KTMREDFDNY
710 720 730 740 750
DFLDAYKVLI NFVNNDLSAF YMNIAKDVLY IEAENSEVRR SMQTVFYDIL
760 770 780 790 800
LTLVKLLTPI LPHTTEEVWS YMNEPEDFVQ LTEIPDPRTF AGEEELLSKW
810 820 830 840 850
DDFMEVRSHV LKSLEEARNA KLIGKSLEAQ VDLYLTDDQK QLLDSLNENI
860 870 880 890 900
QLLLGVSALH VHPADEAPAD ADQYNDGVAV KVTTANGETC ARCRMVKEDV
910 920 930
GSDPAYPELC ARCAAIVREN FPETAEDGLE E
Length:931
Mass (Da):106,698
Last modified:June 10, 2008 - v1
Checksum:i187428AD377459BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP007281 Genomic DNA. Translation: BAG25094.1.
RefSeqiWP_012390521.1. NC_010609.1.
YP_001841574.1. NC_010609.1.

Genome annotation databases

EnsemblBacteriaiBAG25094; BAG25094; LAR_0578.
GeneIDi6230512.
KEGGilrf:LAR_0578.
PATRICi22258491. VBILacReu111271_0618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP007281 Genomic DNA. Translation: BAG25094.1 .
RefSeqi WP_012390521.1. NC_010609.1.
YP_001841574.1. NC_010609.1.

3D structure databases

ProteinModelPortali B2G6L2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 557433.LAR_0578.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAG25094 ; BAG25094 ; LAR_0578 .
GeneIDi 6230512.
KEGGi lrf:LAR_0578.
PATRICi 22258491. VBILacReu111271_0618.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LREU557433:GHNR-625-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative genome analysis of Lactobacillus reuteri and Lactobacillus fermentum reveal a genomic island for reuterin and cobalamin production."
    Morita H., Toh H., Fukuda S., Horikawa H., Oshima K., Suzuki T., Murakami M., Hisamatsu S., Kato Y., Takizawa T., Fukuoka H., Yoshimura T., Itoh K., O'Sullivan D.J., McKay L.L., Ohno H., Kikuchi J., Masaoka T., Hattori M.
    DNA Res. 15:151-161(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 1112.

Entry informationi

Entry nameiSYI_LACRJ
AccessioniPrimary (citable) accession number: B2G6L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3